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We aimed to study the cultivable Actinomycetota community associated with a marine sponge from the Portuguese coast, Hymeniacidon perlevis, and investigate the potential of the retrieved isolates to produce compounds with antimicrobial, anticancer and anti-obesity properties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>The analysis of the 16S rRNA gene revealed 79 Actinomycetota isolates affiliated with 12 genera\u2014Brachybacterium, Dietzia, Glutamicibacter, Gordonia, Micrococcus, Micromonospora, Nocardia, Nocardiopsis, Paenoartrhobacter, Rhodococcus, Streptomyces, and Tsukamurella, most of which affiliated with the genus Streptomyces. The screening of antimicrobial activity revealed 13 strains, all belonging to the Streptomyces genus, capable of inhibiting the growth of Candida albicans, Bacillus subtilis, or Staphylococcus aureus. Forty-three extracts exhibited cytotoxic activity against at least one tested cell line (HepG2, HCT-116, and hCMEC-D3). Three extracts that were active against the two cancer cell lines tested, did not reduce the viability of the non-cancer endothelial cell line, hCMEC-D3. One Gordonia strain exhibited anti-obesity activity, revealed by its ability to reduce the neutral lipids in zebrafish larvae. Mass spectrometry-based dereplication analysis of active extracts identified several compounds associated with known Actinomycetota natural products. Nonetheless, five clusters contained metabolites that did not match any annotated natural products, suggesting they may represent new bioactive molecules.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This work contributed to increase the knowledge on the diversity and bioactive potential of Actinomycetota associated with H. perlevis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf044","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T15:26:32Z","timestamp":1740065192000},"source":"Crossref","is-referenced-by-count":2,"title":["Actinomycetota isolated from the sponge\n                    <i>Hymeniacidon perlevis<\/i>\n                    as a source of novel compounds with pharmacological applications: diversity, bioactivity screening, and metabolomic analysis"],"prefix":"10.1093","volume":"136","author":[{"given":"Ana C","family":"Fonseca","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]},{"name":"FCUP\u2014Faculty of Sciences, University of Porto , Rua do Campo Alegre s\/n 4169-007 Porto ,","place":["Portugal"]}]},{"given":"In\u00eas","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]}]},{"given":"Mariana","family":"Gir\u00e3o","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]}]},{"given":"Ana","family":"Regueiras","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]}]},{"given":"Ralph","family":"Urbatzka","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5064-9164","authenticated-orcid":false,"given":"Pedro","family":"Le\u00e3o","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]}]},{"given":"Maria F","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Porto ,","place":["Portugal"]},{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto , Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto ,","place":["Portugal"]}]}],"member":"286","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"2026022020020700100_bib1","doi-asserted-by":"publisher","first-page":"e0216737","DOI":"10.1371\/journal.pone.0216737","article-title":"Prodigiosins from a marine sponge-associated actinomycete attenuate HCl\/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms","volume":"14","author":"Abdelfattah","year":"2019","journal-title":"PLoS One"},{"key":"2026022020020700100_bib2","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1007\/s13205-018-1304-1","article-title":"Heliomycin and tetracinomycin D: anthraquinone derivatives with histone deacetylase inhibitory activity from marine sponge-associated Streptomyces sp","volume":"8","author":"Abdelfattah","year":"2018","journal-title":"3 Biotech"},{"key":"2026022020020700100_bib3","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.1021\/np800281f","article-title":"Moromycins a and B, isolation and structure elucidation of C-glycosylangucycline-type antibiotics from Streptomyces sp. 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