{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:54:03Z","timestamp":1753876443345,"version":"3.41.2"},"reference-count":101,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Contrato-Programa","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"AgrifoodXXI","award":["NORTE-01-0145-FEDER-000041"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000041"]}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Fungal infections are a major threat to crop productivity and quality. Synthetic antifungals have been used ensuring high production; however, the environmental negative effects and the induction of resistant strains have been rising concerns about their use. Thus, this work aims to valorize Medicago sp. extracts, as potentially safe and effective antifungals in agriculture.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>The chemical analysis of an ethanolic extract showed mainly flavonoids, with flavones (23.18\u00a0\u00b1\u00a00.08\u00a0mg g\u22121) and isoflavones (13.47\u00a0\u00b1\u00a00.11\u00a0mg g\u22121) as the main constituents. Dose-dependent cytotoxicity was observed against Saccharomyces cerevisiae, with concomitant loss of plasma membrane integrity, assessed with propidium iodide, and nucleus-cytosolic translocation of the necrosis marker Nhp6A. Extract\u2019s cytotoxicity in the mutant strain yca1, affected in an apoptosis-related metacaspase, further suggested a necrotic mode of cell death. Significant in vitro antifungal activity was observed against Botrytis cinerea, Colletotrichum acutatum, Colletotrichum gloeosporioides, Colletotrichum godetiae, Colletotrichum nymphaea, and Diplodia corticola, all phytopathogenic fungi with devastating impact on relevant crops worldwide.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The results indicate a remarkable antifungal activity by Medicago sp. extract with potential use in the protection of crops by inducing necrosis in fungal phytopathogens.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf116","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T14:44:29Z","timestamp":1748443469000},"source":"Crossref","is-referenced-by-count":0,"title":["Necrosis-mediated antifungal activity of a <i>Medicago<\/i> sp. extract with high potential as safe antifungal against phytopathogenic fungi"],"prefix":"10.1093","volume":"136","author":[{"given":"T\u00e2nia D","family":"Tavares","sequence":"first","affiliation":[{"name":"Center for Molecular and Environmental Biology (CBMA), University of Minho Department of Biology, , Campus de Gualtar 4710-057 Braga ,","place":["Portugal"]},{"name":"Center for Textile Science and Technology (2C2T), University of Minho Department of Textile Engineering, , Campus de Azur\u00e9m 4800-058 Guimar\u00e3es ,","place":["Portugal"]}]},{"given":"Luara","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Center for Molecular and Environmental Biology (CBMA), University of Minho Department of Biology, , Campus de Gualtar 4710-057 Braga ,","place":["Portugal"]},{"name":"Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es , Avenida General Norton de Matos, 4450-208 Matosinhos 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