{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T03:08:43Z","timestamp":1770520123058,"version":"3.49.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/00100\/2020"],"award-info":[{"award-number":["UIDB\/00100\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UID\/MULTI\/04046\/2019"],"award-info":[{"award-number":["UID\/MULTI\/04046\/2019"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/04292\/2020"],"award-info":[{"award-number":["UIDB\/04292\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The emergence of potentially dangerous new psychoactive substances (NPS) is challenging for forensic laboratories, as well as the ability to develop and validate methods for a rapid and unambiguous monitoring of these compounds. Thereupon, the aim of this work was to establish a methodology for the identification and quantification of four synthetic cathinones already seized in Portugal [4-chloroethcathinone (4-CEC), \u03b1-pyrrolidinovalerophenone (\u03b1-PVP), 4-chloro-pyrrolidinovalerophenone (4-Cl-PVP) and methylenedioxypyrovalerone (MDPV)] in whole blood samples, using gas chromatography coupled to mass spectrometry (GC\u2013MS). The analytes were extracted from blood by solid-phase extraction (SPE) and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) with 5% trimethylchlorosilane (TMCS). For all analytes, linearity was observed from 25 (lower limit of quantification, LLOQ) to 800\u00a0ng\/mL, with coefficients of determination higher than 0.99. The limits of detection (LOD) were 5\u00a0ng\/mL for \u03b1-PVP, 4-Cl-PVP and MDPV and 25\u00a0ng\/mL for 4-CEC. The method was selective, precise and accurate, and the extraction efficiency was higher than 85% for all analytes. The target cathinones were stable under different stock conditions, being MDPV the most stable and 4-CEC the least stable compound. The validated analytical method was then applied to real samples that previously tested positive for amphetamines, but no positive samples were found for the cathinones under study. The present method describes for the first time the quantification of 4-CEC and 4-Cl-PVP in whole blood samples by GC\u2013MS, allowing their accurate determination in forensic situations where the compounds are involved.<\/jats:p>","DOI":"10.1093\/jat\/bkaa074","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T19:11:27Z","timestamp":1592421087000},"page":"233-242","source":"Crossref","is-referenced-by-count":24,"title":["Determination of Selected Cathinones in Blood by Solid-Phase Extraction and GC\u2013MS"],"prefix":"10.1093","volume":"45","author":[{"given":"M\u00f3nica","family":"Antunes","sequence":"first","affiliation":[{"name":"Faculty of Sciences, BioISI\u2014Biosystems & Integrative Sciences Institute, University of Lisboa, Campo Grande, C8, Lisboa 1749-016, Portugal"}]},{"given":"Margarida","family":"Sequeira","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, BioISI\u2014Biosystems & Integrative Sciences Institute, University of Lisboa, Campo Grande, C8, Lisboa 1749-016, Portugal"}]},{"given":"Mariana","family":"de Caires Pereira","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, BioISI\u2014Biosystems & Integrative Sciences Institute, University of Lisboa, Campo Grande, C8, Lisboa 1749-016, Portugal"},{"name":"Laborat\u00f3rio de Pol\u00edcia Cient\u00edfica da Pol\u00edcia Judici\u00e1ria\u2014Setor de Drogas e Toxicologia, Rua Gomes Freire, Lisboa 1169-007, Portugal"}]},{"given":"Maria Jo\u00e3o","family":"Caldeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pol\u00edcia Cient\u00edfica da Pol\u00edcia Judici\u00e1ria\u2014Setor de Drogas e Toxicologia, Rua Gomes Freire, Lisboa 1169-007, Portugal"}]},{"given":"Susana","family":"Santos","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Centro de Qu\u00edmica Estrutural, Universidade de Lisboa, Campo Grande, Lisboa 1749-016, Portugal"}]},{"given":"Jo\u00e3o","family":"Franco","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Medicina Legal e Ci\u00eancias Forenses, I.P.\u2014Delega\u00e7\u00e3o do Sul, Servi\u00e7o de Qu\u00edmica e Toxicologia Forenses, Rua Manuel Bento de Sousa, 3, Lisboa 1150-219, Portugal"}]},{"given":"M\u00e1rio","family":"Barroso","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Medicina Legal e Ci\u00eancias Forenses, I.P.\u2014Delega\u00e7\u00e3o do Sul, Servi\u00e7o de Qu\u00edmica e Toxicologia Forenses, Rua Manuel Bento de Sousa, 3, Lisboa 1150-219, Portugal"}]},{"given":"Helena","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, BioISI\u2014Biosystems & Integrative Sciences Institute, University of Lisboa, Campo Grande, C8, Lisboa 1749-016, Portugal"},{"name":"MARE\u2014Marine and Environmental Sciences Centre, Polytechnic of Leiria, Edif\u00edcio CETEMARES, Avenida do Porto de Pesca, 2520-630 PEniche, 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