{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:08:34Z","timestamp":1777547314441,"version":"3.51.4"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A group of 16 volatile substances (ethyl acetate, 2-propanol, 1-propanol, methanol, acetone, ethanol, acetaldehyde, diethyl ether, methyl ethyl ketone, 1-butanol, 2-butanol, t-butanol, isobutanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 1-pentanol) were qualitatively and quantitatively analyzed through a method developed for volatiles with endogenous production in putrefaction and submersion situations. The method was validated for blood, urine and vitreous humor, using a gas chromatograph (Varian 450-GC) with a flame ionization detector coupled to a headspace injector (HS-GC\u2013FID). The vials were prepared by diluting 100 \u00b5L of the sample of interest in 1 mL of internal standard (acetonitrile 100 mg\/L), using two capillary columns (VF-624ms and VF-5ms) with different polarities to ensure that all test compounds would be properly identified and undoubtedly distinguished from the rest. All volatiles were studied in a range of 50 to 2,000 mg\/L in terms of selectivity\/specificity, detection and quantification limits, linearity and calibration model, precision, accuracy, bias, robustness and stability according to the Scientific Working Group for Forensic Toxicology. Detection and quantification limits were between 1 to 8 mg\/L and 4 to 24 mg\/L, respectively, with coefficient of variation values under 10% in bias studies and in intermediate precision studies for most substances. The developed method was applied to real cases to test the method.<\/jats:p>","DOI":"10.1093\/jat\/bkaa154","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T19:14:33Z","timestamp":1602011673000},"page":"961-968","source":"Crossref","is-referenced-by-count":4,"title":["Development and Validation of an Analytical Method for Volatiles with Endogenous Production in Putrefaction and Submersion Situations"],"prefix":"10.1093","volume":"45","author":[{"given":"M\u00e9lanie","family":"Pinto","sequence":"first","affiliation":[{"name":"National Institute of Legal Medicine and Forensic Sciences, Forensic Chemistry and Toxicology Service, Centre Branch, Largo da S\u00e9 Nova, 3000-213 Coimbra, Portugal"},{"name":"Chemistry Department of the Faculty of Sciences and Technologies, University of Coimbra, Largo da S\u00e9 Nova, 3000-213 Coimbra, Portugal"}]},{"given":"Ermelinda","family":"Eus\u00e9bio","sequence":"additional","affiliation":[{"name":"Chemistry Department of the Faculty of Sciences and Technologies, University of Coimbra, Largo da S\u00e9 Nova, 3000-213 Coimbra, Portugal"}]},{"given":"Carla","family":"Monteiro","sequence":"additional","affiliation":[{"name":"National Institute of Legal Medicine and Forensic Sciences, Forensic Chemistry and Toxicology Service, Centre Branch, Largo da S\u00e9 Nova, 3000-213 Coimbra, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"2021110915564382800_R1","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1097\/00000433-199603000-00002","article-title":"Post-mortem production of ethanol and factors that influence interpretation: a critical review","volume":"17","author":"O\u2019Neal","year":"1996","journal-title":"The American Journal of Forensic Medicine and Pathology"},{"key":"2021110915564382800_R2","first-page":"54","author":"Alcohol and Violence. 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