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Recently, we have shown that the simultaneous activation of nonmucosal DCs through TLR2 and TLR4 maintains such capacity while reinforcing the polarization of primed CD4+ T cells towards Th1. Here, we observed that TLR2\/TLR4 stimulation of aged DCs leads to the production of less TNF\u03b1 and more IL-10 and that CD4+ T cells primed by those DCs express lower levels of the mucosal homing receptor CCR9 and produce less type-1 (IFN\u03b3) and more type-2 (IL-4 and IL-13) cytokines. These results emphasize the importance of considering the age-related alterations in DC function when developing novel immunomodulation strategies that rely on the DC-T cell crosstalk through stimulation of pattern recognition receptors.<\/jats:p>","DOI":"10.1093\/jleuko\/qiaf096","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T06:38:31Z","timestamp":1751006311000},"source":"Crossref","is-referenced-by-count":0,"title":["Ageing affects the CD4+ T cell polarization and mucosal tropism induced by TLR2\/TLR4-activated dendritic cells"],"prefix":"10.1093","volume":"117","author":[{"given":"Sara","family":"Z\u00faquete","sequence":"first","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS) , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]}]},{"given":"Mariana","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS) , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]}]},{"given":"In\u00eas L S","family":"Delgado","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS) , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"I-MVET (Investiga\u00e7\u00e3o em Medicina Veterin\u00e1ria), Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona\u2014Centro Universit\u00e1rio de Lisboa , Campo Grande, N.\u00ba 376, Lisboa 1749-024 ,","place":["Portugal"]}]},{"given":"Maria Teresa","family":"Rosa","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"Faculdade de Ci\u00eancia e Tecnologia, Universidade Nova de Lisboa , Campus da Caparica, Caparica 2829-516 ,","place":["Portugal"]}]},{"given":"Ana Catarina","family":"Mendes","sequence":"additional","affiliation":[{"name":"GIMM\u2014Gulbenkian Institute for Molecular Medicine , Avenida Professor Egas Moniz, Lisbon 1649-028 ,","place":["Portugal"]},{"name":"Faculdade de Medicina, Universidade de Lisboa , Avenida Professor Egas Moniz, Lisbon 1649-028 ,","place":["Portugal"]}]},{"given":"Dulce","family":"Santos","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]}]},{"given":"Sofia","family":"Nolasco","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS) , Avenida da Universidade T\u00e9cnica, Lisboa 1300-477 ,","place":["Portugal"]},{"name":"Escola Superior de Tecnologia da Sa\u00fade de Lisboa, Instituto Polit\u00e9cnico de Lisboa , Avenida D. 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