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In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinh\u00e3o accumulated &amp;gt;0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed that VvNIP2;1 was highly expressed in roots and in green berries. The transient transformation of tobacco leaf epidermal cells mediated by Agrobacterium tumefaciens confirmed VvNIP2;1 localization at the plasma membrane. Transport experiments in oocytes showed that VvNIP2;1 mediates Si and arsenite uptake, whereas permeability studies revealed that VvNIP2;1 expressed in yeast is unable to transport water and glycerol. Si supplementation to pigmented grape cultured cells (cv. Gamay Fre\u00e1ux) had no impact on the total phenolic and anthocyanin content, or on the growth rate and VvNIP2;1 expression. Long-term experiments should help determine the extent of Si uptake over time and whether grapevine can benefit from Si fertilization.<\/jats:p>","DOI":"10.1093\/jxb\/eraa294","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T19:13:09Z","timestamp":1592421189000},"page":"6789-6798","source":"Crossref","is-referenced-by-count":36,"title":["The grapevine NIP2;1 aquaporin is a silicon channel"],"prefix":"10.1093","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4809-6609","authenticated-orcid":false,"given":"Henrique","family":"Noronha","sequence":"first","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real, Portugal"}]},{"given":"Ang\u00e9lica","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal"}]},{"given":"Namiki","family":"Mitani-Ueno","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, Japan"}]},{"given":"Carlos","family":"Conde","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-989X","authenticated-orcid":false,"given":"Farzana","family":"Sabir","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal"},{"name":"LEAF, Linking Landscape, Environment, Agriculture and Food, and DRAT, Departamento de Recursos Biol\u00f3gicos, Ambiente e Territ\u00f3rio, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, Portugal"}]},{"given":"Catarina","family":"Prista","sequence":"additional","affiliation":[{"name":"LEAF, Linking Landscape, Environment, Agriculture and Food, and DRAT, Departamento de Recursos Biol\u00f3gicos, Ambiente e Territ\u00f3rio, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, Portugal"}]},{"given":"Gra\u00e7a","family":"Soveral","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal"}]},{"given":"Paul","family":"Isenring","sequence":"additional","affiliation":[{"name":"Nephrology Group, L\u2019H\u00f4tel-Dieu de Qu\u00e9bec Institution, Department of Medicine, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, Qu\u00e9bec, Canada"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3411-827X","authenticated-orcid":false,"given":"Jian Feng","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, Japan"}]},{"given":"Richard R","family":"B\u00e9langer","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Phytologie, Facult\u00e9 des Sciences de l\u2019Agriculture et de l\u2019Alimentation (FSAA), Universit\u00e9 Laval, Qu\u00e9bec, Qu\u00e9bec, Canada"}]},{"given":"Hern\u00e2ni","family":"Ger\u00f3s","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real, Portugal"},{"name":"Centre of Biological Engineering (CEB), Department of Engineering, 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