{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:51:24Z","timestamp":1782237084874,"version":"3.54.5"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["211\/744-1"],"award-info":[{"award-number":["211\/744-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,19]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Taraxacum koksaghyz has been identified as one of the most promising alternative rubber crops. Its high-quality rubber is produced in the latex of laticifers, a specialized cell type that is organized in a network of elongated tubules throughout the entire plant body. In order to gain insights into the physiological role(s) of latex and hence laticifer biology, we examine the effects of barnase-induced latex RNA degradation on the metabolite and protein compositions in the roots. We established high-quality datasets that enabled precise discrimination between cellular and physiological processes in laticifers and non-laticifer cell types of roots at different vegetative stages. We identified numerous latex-specific proteins, including a perilipin-like protein that has not been studied in plants yet. The barnase-expressing plants revealed a phenotype that did not exude latex, which may provide a valuable genetic basis for future studies of plant\u2013environment interactions concerning latex and also help to clarify the evolution and arbitrary distribution of latex throughout the plant kingdom. The overview of temporal changes in composition and protein abundance provided by our data opens the way for a deeper understanding of the molecular interactions, reactions, and network relationships that underlie the different metabolic pathways in the roots of this potential rubber crop.<\/jats:p>","DOI":"10.1093\/jxb\/erz512","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T12:10:39Z","timestamp":1573647039000},"page":"1278-1293","source":"Crossref","is-referenced-by-count":25,"title":["Comparative proteome and metabolome analyses of latex-exuding and non-exuding Taraxacum koksaghyz roots provide insights into laticifer biology"],"prefix":"10.1093","volume":"71","author":[{"given":"Vincent Alexander","family":"Benninghaus","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, IME, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"van Deenen","sequence":"first","affiliation":[{"name":"Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boje","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, IME, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Uwe","family":"Roelfs","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, IME, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Lassowskat","sequence":"first","affiliation":[{"name":"Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Finkemeier","sequence":"first","affiliation":[{"name":"Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Pr\u00fcfer","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, IME, Muenster, Germany"},{"name":"Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6398-2265","authenticated-orcid":false,"given":"Christian","family":"Schulze Gronover","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, IME, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"2020021921300726900_CIT0001","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Analytical Biochemistry"},{"key":"2020021921300726900_CIT0002","first-page":"1032","article-title":"Novel insights into the organization of laticifer cells: a cell comprising a unified whole system","volume":"172","author":"Castelblanque","year":"2016","journal-title":"Plant Physiology"},{"key":"2020021921300726900_CIT0003","doi-asserted-by":"crossref","first-page":"12780","DOI":"10.3390\/ijms140612780","article-title":"Sesquiterpenoids lactones: benefits to plants and people","volume":"14","author":"Chadwick","year":"2013","journal-title":"International Journal of Molecular Sciences"},{"key":"2020021921300726900_CIT0004","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.phytochem.2012.04.015","article-title":"Altered levels of the Taraxacum kok-saghyz (Russian dandelion) small rubber particle protein, TkSRPP3, result in qualitative and quantitative changes in rubber metabolism","volume":"79","author":"Collins-Silva","year":"2012","journal-title":"Phytochemistry"},{"key":"2020021921300726900_CIT0005","volume-title":"Physiology of rubber tree latex. 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