{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T15:24:04Z","timestamp":1772810644653,"version":"3.50.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BII-BIO\/31\u2009761\/2017"],"award-info":[{"award-number":["PTDC\/BII-BIO\/31\u2009761\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Selected Saccharomyces cerevisiae strains, such as the commercial Ethanol-Red (ER) strain, are used as starters in the bioethanol industry. Yet, bioethanol fermentations are prone to microbial contaminations, mainly by Brettanomyces bruxellensis and lactic acid bacteria. Chemicals, such as sulphuric acid and antibiotics, are commonly used to combat those contaminations, but they have negative environmental impacts. Recently, ER strain was found to secrete antimicrobial peptides (AMPs) active against B. bruxellensis. Therefore, the partial TDH1 and TDH2\/3 genes sequences that codify those AMPs were inserted into the pSR41k plasmid and cloned in ER strains. The relative expression levels (plasmidic\/genomic) of those sequences in the respective modified ER strains were quantified by real-time quantitative polimerase chain reaction (RT-qPCR), confirming their overexpression. The effect of the modified strains on B. bruxellensis (Bb) growth was then evaluated during synthetic must (SM) and carob syrup (CS) fermentations, co-inoculated with 105 cells ml\u22121 of ER and Bb in SM and with 106 of ER and 5\u00a0\u00d7\u00a0103 cells ml\u22121 of Bb in CS. Results showed that modified ER strains exerted a much higher inhibitory effect against B. bruxellensis (72-fold in SM and 10-fold in CS) than the non-modified ER strain. In those fermentations, 90\u2013100\u00a0g l\u22121 of ethanol was produced in 3\u20136 days.<\/jats:p>","DOI":"10.1093\/lambio\/ovad141","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T06:33:58Z","timestamp":1703054038000},"source":"Crossref","is-referenced-by-count":2,"title":["Effect of overexpression of partial <i>TDH1<\/i> and <i>TDH2\/3<\/i> gene sequences in a starter strain of industrial bioethanol fermentation on the <i>Brettanomyces\u2009bruxellensis<\/i> contaminant growth"],"prefix":"10.1093","volume":"76","author":[{"given":"Patr\u00edcia","family":"Branco","sequence":"first","affiliation":[{"name":"Unit of Bioenergy and Biorefinery, LNEG , Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisboa , Portugal"},{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, University of Lisbon , Tapada da Ajuda, 1349-017 Lisboa , Portugal"},{"name":"Biomedical Research Group (BioRG), School of Engineering, Lus\u00f3fona University , 1749-024 Lisboa , Portugal"}]},{"given":"Lu\u00edsa","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, University of Lisbon , Tapada da Ajuda, 1349-017 Lisboa , Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9307-1905","authenticated-orcid":false,"given":"Catarina","family":"Prista","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, University of Lisbon , Tapada da Ajuda, 1349-017 Lisboa , Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9062-9827","authenticated-orcid":false,"given":"Helena","family":"Albergaria","sequence":"additional","affiliation":[{"name":"Unit of Bioenergy and Biorefinery, LNEG , Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisboa , Portugal"}]}],"member":"286","published-online":{"date-parts":[[2023,12,19]]},"reference":[{"key":"2023122323574296300_bib1","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1007\/s00253-015-7255-0","article-title":"Dominance of Saccharomyces cerevisiae in alcoholic fermentation processes: role of physiological fitness and microbial interactions","volume":"100","author":"Albergaria","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"2023122323574296300_bib2","first-page":"98","article-title":"Dominance of Saccharomyces cerevisiae in wine fermentations: secretion of antimicrobial peptides and microbial interactions","volume-title":"Proc. 2nd Int. 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