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Filamentation is an important virulence factor of C. albicans, and RAS1 and RIM101 genes are involved in its regulation. Thus, the main goal of this work was to project ASOs, based on 2'-OMethyl chemical modification, to target RAS1 and RIM101 mRNA and to validate its application either alone or in combination, to reduce Candida filamentation in different human body fluids.<\/jats:p>\n               <jats:p>It was verified that both, anti-RAS1 2'OMe and anti-RIM101 2'OMe oligomers, were able to reduce the levels of RAS1 and RIM101 genes\u2019 expression and to significantly reduce C. albicans filamentation. Furthermore, the combined application of anti-RAS1 2'OMe oligomer and anti-RIM101 2'OMe oligomer enhances the control of C. albicans filamentation in artificial saliva and urine.<\/jats:p>\n               <jats:p>Our work confirms that ASOs are useful tools for research and therapeutic development on the control of candidiasis.<\/jats:p>","DOI":"10.1093\/mmy\/myab033","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T11:54:34Z","timestamp":1622721274000},"page":"1024-1031","source":"Crossref","is-referenced-by-count":3,"title":["The combined application of the anti-<i>RAS1<\/i> and anti-<i>RIM101<\/i> 2'-<i>O<\/i>MethylRNA oligomers enhances <i>Candida albicans<\/i> filamentation control"],"prefix":"10.1093","volume":"59","author":[{"given":"Ana","family":"Barbosa","sequence":"first","affiliation":[{"name":"LIBRO - Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira, CEB - Centre of Biological Engineering, University of Minho , 4710-057 Braga, Portugal"}]},{"given":"Daniela","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"LIBRO - 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