{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T19:57:16Z","timestamp":1778097436357,"version":"3.51.4"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T00:00:00Z","timestamp":1708992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2022.15408.BD"],"award-info":[{"award-number":["2022.15408.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2021.09461.BD"],"award-info":[{"award-number":["2021.09461.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Enterocytozoon bieneusi is a microsporidia commonly found in the gastrointestinal tract of humans and a wide range of other animals, constituting a major cause of microsporidiosis in humans. Although E. bieneusi has been detected in humans, domestic, and wild animals in Portugal, and its presence in bats has been linked to zoonotic characteristics, its occurrence in bats within the country has not been reported. In this study, we investigated the presence of E. bieneusi in 380 bat fecal samples collected in mainland Portugal through a nested PCR assay targeting the internal transcribed spacer region and the flanking small and large subunits of the ribosomal RNA. Enterocytozoon bieneusi was detected in one bat sample (i.e., 0.26%; Pipistrellus pipistrellus). Additionally, another sample tested positive for Enterocytozoon sp. Phylogenetic analysis of the obtained ITS sequence of E. bieneusi revealed clustering within the potentially zoonotic Group 1. This study represents the first report of E. bieneusi in a bat from Europe. Findings presented here contribute to an enhanced understanding of E. bieneusi epidemiology.<\/jats:p>","DOI":"10.1093\/mmy\/myae019","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T07:10:33Z","timestamp":1709104233000},"source":"Crossref","is-referenced-by-count":3,"title":["Molecular detection of <i>Enterocytozoon bieneusi<\/i> in bats from Portugal"],"prefix":"10.1093","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0091-2013","authenticated-orcid":false,"given":"Andreia V S","family":"Cruz","sequence":"first","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto , Porto , Portugal"}]},{"given":"Pedro","family":"L\u00f3pez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Grupo de Virolog\u00eda Cl\u00ednica y Zoonosis, Unidad de Enfermedades Infecciosas, Instituto Maim\u00f3nides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba, Hospital Reina Sof\u00eda, Universidad de C\u00f3rdoba , C\u00f3rdoba , Spain"},{"name":"CIBERINFEC, ISCIII - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III , Madrid , Spain"}]},{"given":"S\u00e9rgio","family":"Santos-Silva","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto , Porto , Portugal"}]},{"given":"Antonio","family":"Rivero-Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Grupo de Virolog\u00eda Cl\u00ednica y Zoonosis, Unidad de Enfermedades Infecciosas, Instituto Maim\u00f3nides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba, Hospital Reina Sof\u00eda, Universidad de C\u00f3rdoba , C\u00f3rdoba , Spain"},{"name":"CIBERINFEC, ISCIII - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III , Madrid , Spain"}]},{"given":"Hugo","family":"Rebelo","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Animal, cE3c \u2013 Centre for Ecology, Evolution and Environmental Changes & CHANGE \u2013 Global Change and Sustainability Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa , Lisboa , Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8769-8103","authenticated-orcid":false,"given":"Jo\u00e3o R","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto , Porto , Portugal"},{"name":"Epidemiology Research Unit (EPIUnit), ISPUP \u2013 Instituto de Sa\u00fade P\u00fablica da Universidade do Porto , Porto , Portugal"},{"name":"Laborat\u00f3rio para a Investiga\u00e7\u00e3o Integrativa e Translacional em Sa\u00fade Populacional (ITR), ISPUP \u2013 Instituto de Sa\u00fade P\u00fablica da Universidade do Porto , 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