{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T08:50:11Z","timestamp":1773219011634,"version":"3.50.1"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay. Oxidative stress-generated DNA lesions can be measured by employing DNA repair enzymes to recognise oxidatively damaged DNA. Unfortunately, there has been a tendency to fail to report results from assay controls (or maybe even not to employ assay controls). We believe this might have been due to uncertainty as to what really constitutes a positive control. It should go without saying that a biomonitoring study cannot have a positive control group as it is unethical to expose healthy humans to DNA damaging (and thus potentially carcinogenic) agents. However, it is possible to include assay controls in the analysis (here meant as a cryopreserved sample of cells i.e. included in each experiment as a reference sample). In the present report we tested potassium bromate (KBrO3) as a positive comet assay control for the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay. Ten laboratories used the same procedure for treatment of monocytic THP-1 cells with KBrO3 (0.5, 1.5 and 4.5 mM for 1 h at 37\u00b0C) and subsequent cryopreservation. Results from one laboratory were excluded in the statistical analysis because of technical issues in the Fpg-modified comet assay. All other laboratories found a concentration\u2013response relationship in cryopreserved samples (regression coefficients from 0.80 to 0.98), although with different slopes ranging from 1.25 to 11.9 Fpg-sensitive sites (%DNA in tail) per 1 mM KBrO3. Our results demonstrate that KBrO3 is a suitable positive comet assay control.<\/jats:p>","DOI":"10.1093\/mutage\/geaa011","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T20:13:31Z","timestamp":1585340011000},"page":"341-348","source":"Crossref","is-referenced-by-count":40,"title":["Potassium bromate as positive assay control for the Fpg-modified comet assay"],"prefix":"10.1093","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-1249","authenticated-orcid":false,"given":"Peter","family":"M\u00f8ller","sequence":"first","affiliation":[{"name":"Department of Public Health, Section of Environmental Health, University of Copenhagen, \u00d8ster Farimagsgade 5A, Copenhagen K, Denmark"}]},{"given":"Damian","family":"Muruzabal","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Navarra, C\/Irunlarrea 1, Pamplona, Spain"}]},{"given":"Tamara","family":"Bakuradze","sequence":"additional","affiliation":[{"name":"Food Chemistry & Toxicology, Department of Chemistry, Technische Universitaet Kaiserslautern, Erwin-Schroedinger-Str. 52, Kaiserslautern, Germany"}]},{"given":"Elke","family":"Richling","sequence":"additional","affiliation":[{"name":"Food Chemistry & Toxicology, Department of Chemistry, Technische Universitaet Kaiserslautern, Erwin-Schroedinger-Str. 52, Kaiserslautern, Germany"}]},{"given":"Ezgi Eyluel","family":"Bankoglu","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, University of W\u00fcrzburg, Versbacher Str. 9, W\u00fcrzburg, Germany"}]},{"given":"Helga","family":"Stopper","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, University of W\u00fcrzburg, Versbacher Str. 9, W\u00fcrzburg, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3288-7331","authenticated-orcid":false,"given":"Sabine A S","family":"Langie","sequence":"additional","affiliation":[{"name":"VITO-Health, Boerentang 200, 2400 Mol, Belgium"},{"name":"Centre for Environmental Sciences, Hasselt University, Agoralaan Gebouw D, 3590 Diepenbeek, Belgium"}]},{"given":"Amaya","family":"Azqueta","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Navarra, C\/Irunlarrea 1, Pamplona, Spain"},{"name":"IdiSNA, Navarra Institute for Health Research, C\/Irunlarrea 3, 31008 Pamplona, Spain"}]},{"given":"Annie","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Public Health, Section of Environmental Health, University of Copenhagen, \u00d8ster Farimagsgade 5A, Copenhagen K, Denmark"}]},{"given":"Francesca","family":"Scavone","sequence":"additional","affiliation":[{"name":"Department NEUROFARBA University of Florence (Section Pharmacology and Toxicology), Viale G. Pieraccini 6, 50134 Florence, Italy"}]},{"given":"Lisa","family":"Giovannelli","sequence":"additional","affiliation":[{"name":"Department NEUROFARBA University of Florence (Section Pharmacology and Toxicology), Viale G. Pieraccini 6, 50134 Florence, Italy"}]},{"given":"Maria","family":"Wojew\u00f3dzka","sequence":"additional","affiliation":[{"name":"Center for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland"}]},{"given":"Marcin","family":"Kruszewski","sequence":"additional","affiliation":[{"name":"Center for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland"},{"name":"Department of Medical Biology and Translational Research, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5572-1089","authenticated-orcid":false,"given":"Vanessa","family":"Valdiglesias","sequence":"additional","affiliation":[{"name":"Universidade da Coru\u00f1a, Grupo DICOMOSA, Centro de Investigaciones Cient\u00edficas Avanzadas (CICA), Departamento de Biolog\u00eda, Facultad de Ciencias, Campus A Zapateira s\/n, 15071, A Coru\u00f1a, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7649-2599","authenticated-orcid":false,"given":"Blanca","family":"Laffon","sequence":"additional","affiliation":[{"name":"Universidade da Coru\u00f1a, Grupo DICOMOSA, Centro de Investigaciones Cient\u00edficas Avanzadas (CICA), Departamento de Psicolog\u00eda, Facultad de Ciencias de la Educaci\u00f3n, Campus Elvi\u00f1a s\/n, 15071, A Coru\u00f1a, Spain"}]},{"given":"Carla","family":"Costa","sequence":"additional","affiliation":[{"name":"Environmental Health Department, Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal"},{"name":"EPIUnit - Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, n\u00ba 135, 4050-600 Porto, Portugal"}]},{"given":"Solange","family":"Costa","sequence":"additional","affiliation":[{"name":"Environmental Health Department, Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal"},{"name":"EPIUnit - Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, n\u00ba 135, 4050-600 Porto, Portugal"}]},{"given":"Jo\u00e3o Paulo","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Environmental Health Department, Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal"},{"name":"EPIUnit - Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, n\u00ba 135, 4050-600 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9913-4380","authenticated-orcid":false,"given":"Mirko","family":"Marino","sequence":"additional","affiliation":[{"name":"Universita\u0300 degli Studi di Milano, Department of Food, Environmental and Nutritional Sciences (DeFENS), Via Giovanni Celoria 2, 20133 Milan, Italy"}]},{"given":"Cristian","family":"Del Bo\u2019","sequence":"additional","affiliation":[{"name":"Universita\u0300 degli Studi di Milano, Department of Food, Environmental and Nutritional Sciences (DeFENS), Via Giovanni Celoria 2, 20133 Milan, Italy"}]},{"given":"Patrizia","family":"Riso","sequence":"additional","affiliation":[{"name":"Universita\u0300 degli Studi di Milano, Department of Food, Environmental and Nutritional Sciences (DeFENS), Via Giovanni Celoria 2, 20133 Milan, Italy"}]},{"given":"Sergey","family":"Shaposhnikov","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of Oslo, Sognsvannsveien 9, 0372, Oslo, Norway"},{"name":"NorGenotech AS, Norway"}]},{"given":"Andrew","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of Oslo, Sognsvannsveien 9, 0372, Oslo, Norway"},{"name":"NorGenotech AS, Norway"}]}],"member":"286","published-online":{"date-parts":[[2020,4,22]]},"reference":[{"key":"2020091205040265800_CIT0001","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/mutage\/gel019","article-title":"hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII","volume":"21","author":"Smith","year":"2006","journal-title":"Mutagenesis"},{"key":"2020091205040265800_CIT0002","first-page":"5","article-title":"Measurement of DNA damage with the comet assay in high-prevalence diseases: current status and future directions","volume":"35","author":"M\u00f8ller","year":"2020","journal-title":"Mutagenesis"},{"key":"2020091205040265800_CIT0003","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1093\/mutage\/gex015","article-title":"Searching for assay controls for the Fpg- and hOGG1-modified comet assay","volume":"33","author":"M\u00f8ller","year":"2018","journal-title":"Mutagenesis"},{"key":"2020091205040265800_CIT0004","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.mrgentox.2019.04.007","article-title":"Technical recommendations to perform the alkaline standard and enzyme-modified comet assay in human biomonitoring studies","volume":"843","author":"Azqueta","year":"2019","journal-title":"Mutat. 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