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Here, we characterized the 3\u2032-5\u2032 exoribonuclease RNase R from the bacterial pathogen Helicobacter pylori. The \u2018prototypical\u2019 Escherichia coli RNase R displays both exoribonuclease and helicase activities, but whether this latter RNA unwinding function is a general feature of bacterial RNase R had not been addressed. We observed that H. pylori HpRNase R protein does not carry the domains responsible for helicase activity and accordingly the purified protein is unable to degrade in vitro RNA molecules with secondary structures. The lack of RNase R helicase domains is widespread among the Campylobacterota, which include Helicobacter and Campylobacter genera, and this loss occurred gradually during their evolution. An in vivo interaction between HpRNase R and RhpA, the sole DEAD-box RNA helicase of H. pylori was discovered. Purified RhpA facilitates the degradation of double stranded RNA by HpRNase R, showing that this complex is functional. HpRNase R has a minor role in 5S rRNA maturation and few targets in H. pylori, all included in the RhpA regulon. We concluded that during evolution, HpRNase R has co-opted the RhpA helicase to compensate for its lack of helicase activity.<\/jats:p>","DOI":"10.1093\/nar\/gkab283","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T19:10:34Z","timestamp":1617995434000},"page":"5249-5264","source":"Crossref","is-referenced-by-count":9,"title":["RNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase in<i>Helicobacter pylori<\/i>"],"prefix":"10.1093","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8452-8245","authenticated-orcid":false,"given":"Alejandro","family":"Tejada-Arranz","sequence":"first","affiliation":[{"name":"Unit\u00e9 Pathogen\u00e8se de Helicobacter, CNRS UMR 2001, D\u00e9partement de Microbiologie, Institut Pasteur, 75724 Paris Cedex 15, France"},{"name":"Universit\u00e9 de Paris, Sorbonne Paris Cit\u00e9, 75006 Paris, France"}]},{"given":"Rute G","family":"Matos","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"given":"Yves","family":"Quentin","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de G\u00e9n\u00e9tique Mol\u00e9culaires (LMGM), Centre de Biologie Int\u00e9grative (CBI), Universit\u00e9 de Toulouse, UMR CNRS 5100, 31062 TOULOUSE Cedex 9, France"}]},{"given":"Maxime","family":"Bouilloux-Lafont","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Pathogen\u00e8se de Helicobacter, CNRS UMR 2001, D\u00e9partement de Microbiologie, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"given":"Elo\u00efse","family":"Galtier","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Pathogen\u00e8se de Helicobacter, CNRS UMR 2001, D\u00e9partement de Microbiologie, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"given":"Val\u00e9rie","family":"Briolat","sequence":"additional","affiliation":[{"name":"Biomics, C2RT, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"given":"Etienne","family":"Kornobis","sequence":"additional","affiliation":[{"name":"Biomics, C2RT, Institut Pasteur, 75724 Paris Cedex 15, France"},{"name":"Hub Bioinformatique et Biostatistique, D\u00e9partement de Biologie Computationelle, USR CNRS 3756, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"given":"Thibaut","family":"Douch\u00e9","sequence":"additional","affiliation":[{"name":"Plateforme Prot\u00e9omique, Unit\u00e9 de Spectrom\u00e9trie de Masse pour la Biologie, C2RT, USR CNRS 2000, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"given":"Mariette","family":"Matondo","sequence":"additional","affiliation":[{"name":"Plateforme Prot\u00e9omique, Unit\u00e9 de Spectrom\u00e9trie de Masse pour la Biologie, C2RT, USR CNRS 2000, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"given":"Cecilia M","family":"Arraiano","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"given":"Bertrand","family":"Raynal","sequence":"additional","affiliation":[{"name":"Plateforme de biophysique mol\u00e9culaire, UMR CNRS 3528, D\u00e9partement de Biologie structurale et chimie, Institut Pasteur, 75724 Paris Cedex 15, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0100-4826","authenticated-orcid":false,"given":"Hilde","family":"De\u00a0Reuse","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Pathogen\u00e8se de Helicobacter, CNRS UMR 2001, D\u00e9partement de Microbiologie, Institut Pasteur, 75724 Paris Cedex 15, France"}]}],"member":"286","published-online":{"date-parts":[[2021,4,24]]},"reference":[{"key":"2021070812012700800_B1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.tibs.2019.10.002","article-title":"Bacterial RNA degradosomes: molecular machines under tight control","volume":"45","author":"Tejada-Arranz","year":"2020","journal-title":"Trends Biochem. 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