{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T01:51:27Z","timestamp":1769737887742,"version":"3.49.0"},"reference-count":93,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness MINECO","award":["AGL2014-52194-R"],"award-info":[{"award-number":["AGL2014-52194-R"]}]},{"name":"Spanish Ministry of Economy and Competitiveness MINECO","award":["RTI2018-097244-B-I00"],"award-info":[{"award-number":["RTI2018-097244-B-I00"]}]},{"DOI":"10.13039\/501100014181","name":"Junta of Extremadura","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014181","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["IB18075"],"award-info":[{"award-number":["IB18075"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission. However, changes in cell wall composition during natural fruit abscission are poorly understood. In olive (Olea europaea L.), some cultivars such as \u2018Picual\u2019 undergo massive natural fruit abscission after fruit ripening. This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in \u2018Picual\u2019 olive. To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ. Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides. Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones. Our results indicate that unesterified HGs are likely to contribute to cell separation in the olive fruit AZ. Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission. In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission. This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.<\/jats:p>","DOI":"10.1093\/pcp\/pcaa009","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T12:07:41Z","timestamp":1580126861000},"page":"814-825","source":"Crossref","is-referenced-by-count":18,"title":["Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission"],"prefix":"10.1093","volume":"61","author":[{"given":"Ruben","family":"Parra","sequence":"first","affiliation":[{"name":"Department of Plant Physiology, Faculty of Science, University of Extremadura, Avda de Elvas s\/n, 06006 Badajoz, Spain"}]},{"given":"Miguel A","family":"Paredes","sequence":"first","affiliation":[{"name":"Department of Plant Physiology, Faculty of Science, University of Extremadura, Avda de Elvas s\/n, 06006 Badajoz, Spain"}]},{"given":"Juana","family":"Labrador","sequence":"first","affiliation":[{"name":"Department of Plant Physiology, Faculty of Science, University of Extremadura, Avda de Elvas s\/n, 06006 Badajoz, Spain"}]},{"given":"Cl\u00e1udia","family":"Nunes","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Aveiro, Aveiro P-3810-193, Portugal"}]},{"given":"Manuel A","family":"Coimbra","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Aveiro, Aveiro P-3810-193, Portugal"}]},{"given":"Nieves","family":"Fernandez-Garcia","sequence":"first","affiliation":[{"name":"Department of Abiotic Stress and Plant Pathology, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CSIC), Murcia, Spain"}]},{"given":"Enrique","family":"Olmos","sequence":"first","affiliation":[{"name":"Department of Abiotic Stress and Plant Pathology, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CSIC), Murcia, Spain"}]},{"given":"Mercedes","family":"Gallardo","sequence":"first","affiliation":[{"name":"Department of Plant Physiology, University of Vigo, Campus Lagoas-Marcosende, s\/n, Vigo 36310, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2977-5814","authenticated-orcid":false,"given":"Maria C","family":"Gomez-Jimenez","sequence":"first","affiliation":[{"name":"Department of Plant Physiology, Faculty of Science, University of Extremadura, Avda de Elvas s\/n, 06006 Badajoz, Spain"}]}],"member":"286","published-online":{"date-parts":[[2020,2,4]]},"reference":[{"key":"2020041412053281100_pcaa009-B1","volume-title":"Abscission","author":"Addicott","year":"1982"},{"key":"2020041412053281100_pcaa009-B2","volume-title":"Plant Cell Walls","author":"Albersheim","year":"2011"},{"key":"2020041412053281100_pcaa009-B3","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/0003-2697(73)90377-1","article-title":"New method for quantitative determination of uronic acids","volume":"54","author":"Blumenkrantz","year":"1973","journal-title":"Anal. 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