{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T10:51:49Z","timestamp":1776941509474,"version":"3.51.4"},"reference-count":106,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T00:00:00Z","timestamp":1689033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Changes in cytosolic calcium (Ca2+) concentration are among the earliest reactions to a multitude of stress cues. While a plethora of Ca2+-permeable channels may generate distinct Ca2+ signatures and contribute to response specificities, the mechanisms by which Ca2+ signatures are decoded are poorly understood. Here, we developed a genetically encoded F\u00f6rster resonance energy transfer (FRET)-based reporter that visualizes the conformational changes in Ca2+-dependent protein kinases (CDPKs\/CPKs). We focused on two CDPKs with distinct Ca2+-sensitivities, highly Ca2+-sensitive Arabidopsis (Arabidopsis thaliana) AtCPK21 and rather Ca2+-insensitive AtCPK23, to report conformational changes accompanying kinase activation. In tobacco (Nicotiana tabacum) pollen tubes, which naturally display coordinated spatial and temporal Ca2+ fluctuations, CPK21-FRET, but not CPK23-FRET, reported oscillatory emission ratio changes mirroring cytosolic Ca2+ changes, pointing to the isoform-specific Ca2+-sensitivity and reversibility of the conformational change. In Arabidopsis guard cells, CPK21-FRET-monitored conformational dynamics suggest that CPK21 serves as a decoder of signal-specific Ca2+ signatures in response to abscisic acid and the flagellin peptide flg22. Based on these data, CDPK-FRET is a powerful approach for tackling real-time live-cell Ca2+ decoding in a multitude of plant developmental and stress responses.<\/jats:p>","DOI":"10.1093\/plcell\/koad196","type":"journal-article","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T19:21:57Z","timestamp":1689103317000},"page":"276-297","source":"Crossref","is-referenced-by-count":26,"title":["Imaging of plant calcium-sensor kinase conformation monitors real time calcium-dependent decoding <i>in planta<\/i>"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4766-2971","authenticated-orcid":false,"given":"Anja","family":"Liese","sequence":"first","affiliation":[{"name":"Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry , D-06120 Halle (Saale) , Germany"},{"name":"Dahlem Centre of Plant Sciences, Freie Universit\u00e4t Berlin , D-14195 Berlin , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7431-8984","authenticated-orcid":false,"given":"Bernadette","family":"Eichst\u00e4dt","sequence":"additional","affiliation":[{"name":"Dahlem Centre of Plant Sciences, Freie Universit\u00e4t Berlin , D-14195 Berlin , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1464-1154","authenticated-orcid":false,"given":"Sarah","family":"Lederer","sequence":"additional","affiliation":[{"name":"Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry , D-06120 Halle (Saale) , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4401-5901","authenticated-orcid":false,"given":"Philipp","family":"Schulz","sequence":"additional","affiliation":[{"name":"Dahlem Centre of Plant Sciences, Freie Universit\u00e4t Berlin , D-14195 Berlin , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7640-5294","authenticated-orcid":false,"given":"Jan","family":"Oehlschl\u00e4ger","sequence":"additional","affiliation":[{"name":"Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry , D-06120 Halle (Saale) , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-2519","authenticated-orcid":false,"given":"Susanne","family":"Matschi","sequence":"additional","affiliation":[{"name":"Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry , D-06120 Halle (Saale) , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1100-5478","authenticated-orcid":false,"given":"Jos\u00e9 A","family":"Feij\u00f3","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Molecular Genetics, University of Maryland, 2136 Bioscience Research Bldg, College Park , MD 20742-5815 , USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-7245","authenticated-orcid":false,"given":"Waltraud X","family":"Schulze","sequence":"additional","affiliation":[{"name":"Plant Systems Biology, Universit\u00e4t Hohenheim , D-70593 Stuttgart , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4626-5429","authenticated-orcid":false,"given":"Kai R","family":"Konrad","sequence":"additional","affiliation":[{"name":"Julius-Von-Sachs Institute for Biosciences, Julius Maximilians Universit\u00e4t W\u00fcrzburg , D-97082 W\u00fcrzburg , Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0838-0031","authenticated-orcid":false,"given":"Tina","family":"Romeis","sequence":"additional","affiliation":[{"name":"Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry , D-06120 Halle (Saale) , Germany"},{"name":"Dahlem Centre of Plant Sciences, Freie Universit\u00e4t Berlin , D-14195 Berlin , 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