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In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike\u00ae) and implanted in goat critical-sized calvarial defects (14\u2009mm) for 3, 6 and 12\u2009weeks. The results showed that HG improved the handling properties of the Bonelike\u00ae granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.<\/jats:p>","DOI":"10.1093\/rb\/rbaa036","type":"journal-article","created":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T17:36:51Z","timestamp":1606585011000},"source":"Crossref","is-referenced-by-count":10,"title":["Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute"],"prefix":"10.1093","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9769-7208","authenticated-orcid":false,"given":"Isabel","family":"Pereira","sequence":"first","affiliation":[{"name":"CEB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal"}]},{"given":"Jos\u00e9 Eduardo","family":"Pereira","sequence":"additional","affiliation":[{"name":"CECAV, Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real 5001-801, Portugal"},{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real 5001-801, Portugal"}]},{"given":"Lu\u00eds","family":"Maltez","sequence":"additional","affiliation":[{"name":"CECAV, Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real 5001-801, Portugal"},{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real 5001-801, Portugal"}]},{"given":"Alexandra","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CEB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal"}]},{"given":"Catarina","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CEB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal"}]},{"given":"Manuela","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CEB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal"}]},{"given":"Dina M","family":"Silva","sequence":"additional","affiliation":[{"name":"Biosckin, Molecular and Cell Therapies S.A., Laborat\u00f3rio Criovida, TecMaia, Rua Engenheiro Frederico Ulrich 2650, Moreira da Maia 4470-605, Portugal"}]},{"given":"Ana Rita","family":"Caseiro","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n\u00b0 228, Porto 4050-313, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, Porto 4051-401 Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o Vasco da Gama (CIVG)\/Escola Universit\u00e1ria Vasco da Gama (EUVG), Avenida Jos\u00e9 R. Sousa Fernandes, n.\u00b0 197 Lordem\u00e3o, Coimbra 3020-210, Portugal"}]},{"given":"Justina","family":"Prada","sequence":"additional","affiliation":[{"name":"CECAV, Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real 5001-801, Portugal"},{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro, Vila Real 5001-801, Portugal"}]},{"given":"Ana Colette","family":"Maur\u00edcio","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n\u00b0 228, Porto 4050-313, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, Porto 4051-401 Portugal"}]},{"given":"Jos\u00e9 Domingos","family":"Santos","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Departamento de Engenharia Metal\u00fargica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr Roberto Frias, Porto 4200-495, Portugal"}]},{"given":"Miguel","family":"Gama","sequence":"additional","affiliation":[{"name":"CEB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2020,11,28]]},"reference":[{"key":"2021031107175550600_rbaa036-B1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/S1369-7021(08)70086-5","article-title":"Biomaterials for bone tissue engineering","volume":"11","author":"Stevens","year":"2008","journal-title":"Mater 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