{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T10:28:49Z","timestamp":1777976929046,"version":"3.51.4"},"reference-count":85,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":78,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality","award":["22520711900"],"award-info":[{"award-number":["22520711900"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52273125"],"award-info":[{"award-number":["52273125"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2232024D-01"],"award-info":[{"award-number":["2232024D-01"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52173031"],"award-info":[{"award-number":["52173031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Oriental Talent Plan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Silk fibroin (SF) hydrogels possess excellent biocompatibility and biomimetic properties of the extracellular matrix. Among them, the mild chemical crosslinked SF hydrogels show great application potential in the fields of 3D cell culture and tissue repairing and thus have attracted widespread attention. However, the mobility of hydrophobic chain segments of SF molecules in these chemical crosslinked hydrogels can easily cause the molecules to undergo a self-assembly process from random coil to \u03b2-sheet conformation due to its lower energy state, thus inducing an inevitable conformational transition process. This process further leads to dynamic changes of important material features, such as the hydrogel pore size and mechanical properties, which can probably bring some non-negligible and unknown impacts on cell behaviors and their biomedical applications. In this study, a typical mild crosslinking system composed of horseradish peroxidase and hydrogen peroxide was chosen to prepare SF hydrogels. A feasible protein conformational transition rate controlling strategy based on hydrogel crosslinking density regulation was also proposed. Our results demonstrate that the lower the hydrogel crosslinking density, the faster the conformational transition rate. Subsequently, SF hydrogels with different conformational transition rates were successfully constructed to investigate the impact of the protein conformational transition rate accompanied with initial crosslinking density on the proliferation and chondrogenic differentiation of encapsulated stem cells. Results comprehensively illustrated that the conformational transition process could effectively regulate cell behavior. The hydrogel with an appropriate conformational transition rate obviously promoted the proliferation and chondrogenesis of encapsulated stem cells, while too fast or too slow transition processes slowed down these cell activities. These findings are hopefully to provide valuable guidance for the development and efficient usage of SF hydrogels in the fields of 3D cell culture and tissue engineering.<\/jats:p>","DOI":"10.1093\/rb\/rbaf019","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T19:05:04Z","timestamp":1742583904000},"source":"Crossref","is-referenced-by-count":8,"title":["Effects of protein conformational transition accompanied with crosslinking density cues in silk fibroin hydrogels on the proliferation and chondrogenesis of encapsulated stem cells"],"prefix":"10.1093","volume":"12","author":[{"given":"Guolong","family":"Cai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Advanced Fiber Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Materials Science and Engineering, Donghua University , Shanghai 201620,","place":["People\u2019s Republic of China"]}]},{"given":"Weikun","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Advanced Fiber Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Materials Science and Engineering, Donghua University , Shanghai 201620,","place":["People\u2019s Republic of China"]}]},{"given":"Tianhao","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Advanced Fiber Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Materials Science and Engineering, Donghua University , Shanghai 201620,","place":["People\u2019s Republic of China"]}]},{"given":"Ana L","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica Portuguesa, CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia , Porto 4169-005,","place":["Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0152-9550","authenticated-orcid":false,"given":"Xiang","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Advanced Fiber Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Materials Science and Engineering, Donghua University , Shanghai 201620,","place":["People\u2019s Republic of China"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7175-6150","authenticated-orcid":false,"given":"Yaopeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Advanced Fiber Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Materials Science and Engineering, Donghua University , Shanghai 201620,","place":["People\u2019s Republic of China"]}]}],"member":"286","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"2025042616415764100_rbaf019-B1","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.tibtech.2018.04.004","article-title":"Overview of silk fibroin use in wound dressings","volume":"36","author":"Farokhi","year":"2018","journal-title":"Trends Biotechnol"},{"key":"2025042616415764100_rbaf019-B2","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1016\/j.progpolymsci.2007.05.013","article-title":"Silk as a biomaterial","volume":"32","author":"Vepari","year":"2007","journal-title":"Prog Polym Sci"},{"key":"2025042616415764100_rbaf019-B3","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.actbio.2020.11.037","article-title":"Protein composites from silkworm cocoons as versatile biomaterials","volume":"121","author":"Wang","year":"2021","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B4","doi-asserted-by":"crossref","first-page":"100381","DOI":"10.1016\/j.mtbio.2022.100381","article-title":"Bioinspired silk fibroin materials: from silk building blocks extraction and reconstruction to advanced biomedical applications","volume":"16","author":"Yao","year":"2022","journal-title":"Mater Today Bio"},{"key":"2025042616415764100_rbaf019-B5","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.actbio.2022.09.021","article-title":"Nonmulberry silk fibroin-based biomaterials: impact on cell behavior regulation and tissue regeneration","volume":"153","author":"Zou","year":"2022","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B6","doi-asserted-by":"crossref","first-page":"rbae068","DOI":"10.1093\/rb\/rbae068","article-title":"Enhancing angiogenesis in peri-implant soft tissue with bioactive silk fibroin microgroove coatings on zirconia surfaces","volume":"11","author":"Wang","year":"2024","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B7","doi-asserted-by":"crossref","first-page":"rbad074","DOI":"10.1093\/rb\/rbad074","article-title":"Incorporation of kartogenin and silk fibroin scaffolds promotes rat articular cartilage regeneration through enhancement of antioxidant functions","volume":"10","author":"Pan","year":"2023","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B8","doi-asserted-by":"crossref","first-page":"15798","DOI":"10.1021\/acsami.3c17879","article-title":"Design of Bombyx mori (B. mori) silk fibroin microspheres for developing biosafe sunscreen","volume":"16","author":"Wang","year":"2024","journal-title":"ACS Appl Mater Interfaces"},{"key":"2025042616415764100_rbaf019-B9","doi-asserted-by":"crossref","first-page":"2401732","DOI":"10.1002\/adfm.202401732","article-title":"Biomimetic design of hydration-responsive silk fibers and their role in actuators and self-modulated textiles","volume":"34","author":"Xu","year":"2024","journal-title":"Adv Funct Mater"},{"key":"2025042616415764100_rbaf019-B10","doi-asserted-by":"crossref","first-page":"990","DOI":"10.3390\/biom13060990","article-title":"Graphene oxide-coated patterned silk fibroin films promote cell adhesion and induce cardiomyogenic differentiation of human mesenchymal stem cells","volume":"13","author":"Wang","year":"2023","journal-title":"Biomolecules"},{"key":"2025042616415764100_rbaf019-B11","doi-asserted-by":"crossref","first-page":"e2200380","DOI":"10.1002\/mabi.202200380","article-title":"Silk fibroin combined with electrospinning as a promising strategy for tissue regeneration","volume":"23","author":"Chen","year":"2023","journal-title":"Macromol Biosci"},{"key":"2025042616415764100_rbaf019-B12","doi-asserted-by":"crossref","first-page":"5514","DOI":"10.1039\/D1TB00944C","article-title":"Electrospun regenerated Antheraea pernyi silk fibroin scaffolds with improved pore size, mechanical properties and cytocompatibility using mesh collectors","volume":"9","author":"Zou","year":"2021","journal-title":"J Mater Chem B"},{"key":"2025042616415764100_rbaf019-B13","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1007\/s42765-024-00395-8","article-title":"Nanofiber scaffold-based tissue engineering for the treatment of acute liver failure","volume":"6","author":"Liu","year":"2024","journal-title":"Adv Fiber Mater"},{"key":"2025042616415764100_rbaf019-B14","doi-asserted-by":"crossref","first-page":"rbae117","DOI":"10.1093\/rb\/rbae117","article-title":"A facile nanopattern modification of silk fibroin electrospun scaffold and the corresponding impact","volume":"11","author":"Liu","year":"2024","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B15","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.actbio.2015.11.034","article-title":"Silk protein-based hydrogels: promising advanced materials for biomedical applications","volume":"31","author":"Kapoor","year":"2016","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B16","doi-asserted-by":"crossref","first-page":"5484","DOI":"10.1038\/s41596-021-00622-1","article-title":"3D bioprinted silk fibroin hydrogels for tissue engineering","volume":"16","author":"Kim","year":"2021","journal-title":"Nature Protoc"},{"key":"2025042616415764100_rbaf019-B17","first-page":"3396","article-title":"3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration","volume":"6","author":"Li","year":"2021","journal-title":"Bioact Mater"},{"key":"2025042616415764100_rbaf019-B18","doi-asserted-by":"crossref","first-page":"111173","DOI":"10.1016\/j.compositesb.2023.111173","article-title":"Silk fibroin\/polyacrylamide-based tough 3D printing scaffold with strain sensing ability and chondrogenic activity","volume":"271","author":"Geng","year":"2024","journal-title":"Compos B Eng"},{"key":"2025042616415764100_rbaf019-B19","doi-asserted-by":"crossref","first-page":"109764","DOI":"10.1016\/j.compositesb.2022.109764","article-title":"Effects of dynamic mechanical stimulations on the regeneration of in vitro and in vivo cartilage tissue based on silk fibroin scaffold","volume":"235","author":"Gu","year":"2022","journal-title":"Compos B Eng"},{"key":"2025042616415764100_rbaf019-B20","doi-asserted-by":"crossref","first-page":"49953","DOI":"10.1021\/acsami.3c07060","article-title":"Glucose-responsive self-regulated injectable silk fibroin hydrogel for controlled insulin delivery","volume":"15","author":"Maity","year":"2023","journal-title":"ACS Appl Mater Interfaces"},{"key":"2025042616415764100_rbaf019-B21","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1002\/bit.22361","article-title":"Hydrogels as extracellular matrix mimics for 3D cell culture","volume":"103","author":"Tibbitt","year":"2009","journal-title":"Biotechnol Bioeng"},{"key":"2025042616415764100_rbaf019-B22","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/j.biomaterials.2007.11.003","article-title":"Sonication-induced gelation of silk fibroin for cell encapsulation","volume":"29","author":"Wang","year":"2008","journal-title":"Biomaterials"},{"key":"2025042616415764100_rbaf019-B23","doi-asserted-by":"crossref","first-page":"21630","DOI":"10.1021\/jp056350v","article-title":"Mechanisms of silk fibroin sol-gel transitions","volume":"110","author":"Matsumoto","year":"2006","journal-title":"J Phys Chem B"},{"key":"2025042616415764100_rbaf019-B24","first-page":"1","article-title":"Research frontiers based on regenerative silk fibroin hydrogels","volume":"39","author":"Long","year":"2018","journal-title":"J Chem Res"},{"key":"2025042616415764100_rbaf019-B25","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1016\/j.actbio.2012.01.004","article-title":"Silk fibroin\/poly (vinyl alcohol) photocrosslinked hydrogels for delivery of macromolecular drugs","volume":"8","author":"Kundu","year":"2012","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B26","doi-asserted-by":"crossref","first-page":"110670","DOI":"10.1016\/j.matdes.2022.110670","article-title":"An injectable and photocurable methacrylate-silk fibroin hydrogel loaded with bFGF for spinal cord regeneration","volume":"217","author":"Zhou","year":"2022","journal-title":"Mater Des"},{"key":"2025042616415764100_rbaf019-B27","doi-asserted-by":"crossref","first-page":"116689","DOI":"10.1016\/j.carbpol.2020.116689","article-title":"Tannic acid-reinforced methacrylated chitosan\/methacrylated silk fibroin hydrogels with multifunctionality for accelerating wound healing","volume":"247","author":"He","year":"2020","journal-title":"Carbohydr Polym"},{"key":"2025042616415764100_rbaf019-B28","doi-asserted-by":"crossref","first-page":"rbab048","DOI":"10.1093\/rb\/rbab048","article-title":"Photocrosslinking silver nanoparticles-aloe vera-silk fibroin composite hydrogel for treatment of full-thickness cutaneous wounds","volume":"8","author":"Liu","year":"2021","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B29","doi-asserted-by":"crossref","first-page":"e2001801","DOI":"10.1002\/adhm.202001801","article-title":"Rapid photocrosslinking of silk hydrogels with high cell density and enhanced shape fidelity","volume":"9","author":"Cui","year":"2020","journal-title":"Adv Healthc Mater"},{"key":"2025042616415764100_rbaf019-B30","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/term.2982","article-title":"Phospholipid-induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration","volume":"14","author":"Laomeephol","year":"2020","journal-title":"J Tissue Eng Regen Med"},{"key":"2025042616415764100_rbaf019-B31","doi-asserted-by":"crossref","first-page":"e2001646","DOI":"10.1002\/adhm.202001646","article-title":"Biomimetic silk fibroin hydrogels strengthened by silica nanoparticles distributed nanofibers facilitate bone repair","volume":"10","author":"Cheng","year":"2021","journal-title":"Adv Healthc Mater"},{"key":"2025042616415764100_rbaf019-B32","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1039\/D3BM01901B","article-title":"A nanoparticle reinforced microporous methacrylated silk fibroin hydrogel to promote bone regeneration","volume":"12","author":"Wang","year":"2024","journal-title":"Biomater Sci"},{"key":"2025042616415764100_rbaf019-B33","doi-asserted-by":"crossref","first-page":"104018","DOI":"10.1016\/j.jddst.2022.104018","article-title":"Composite silk fibroin hydrogel scaffolds for cartilage tissue regeneration","volume":"79","author":"Montaseri","year":"2023","journal-title":"J Drug Deliv Sci Technol"},{"key":"2025042616415764100_rbaf019-B34","doi-asserted-by":"crossref","first-page":"119679","DOI":"10.1016\/j.biomaterials.2019.119679","article-title":"Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering","volume":"232","author":"Hong","year":"2020","journal-title":"Biomaterials"},{"key":"2025042616415764100_rbaf019-B35","doi-asserted-by":"crossref","first-page":"115335","DOI":"10.1016\/j.carbpol.2019.115335","article-title":"Enhanced dual network hydrogels consisting of thiolated chitosan and silk fibroin for cartilage tissue engineering","volume":"227","author":"Liu","year":"2020","journal-title":"Carbohydr Polym"},{"key":"2025042616415764100_rbaf019-B36","doi-asserted-by":"crossref","first-page":"e2200602","DOI":"10.1002\/adhm.202200602","article-title":"Nanosilicate-reinforced silk fibroin hydrogel for endogenous regeneration of both cartilage and subchondral bone","volume":"11","author":"Sheng","year":"2022","journal-title":"Adv Healthc Mater"},{"key":"2025042616415764100_rbaf019-B37","doi-asserted-by":"crossref","first-page":"100962","DOI":"10.1016\/j.mtbio.2024.100962","article-title":"Injectable silk fibroin peptide nanofiber hydrogel composite scaffolds for cartilage regeneration","volume":"25","author":"Wu","year":"2024","journal-title":"Mater Today Bio"},{"key":"2025042616415764100_rbaf019-B38","doi-asserted-by":"crossref","first-page":"7531","DOI":"10.1021\/acsami.1c11840","article-title":"Bioinspired injectable hydrogels dynamically stiffen and contract to promote mechanosensing-mediated chondrogenic commitment of stem cells","volume":"14","author":"Mahajan","year":"2022","journal-title":"ACS Appl Mater Interfaces"},{"key":"2025042616415764100_rbaf019-B39","doi-asserted-by":"crossref","first-page":"e0194441","DOI":"10.1371\/journal.pone.0194441","article-title":"Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death","volume":"13","author":"Ribeiro","year":"2018","journal-title":"PLoS One"},{"key":"2025042616415764100_rbaf019-B40","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s12951-021-01230-7","article-title":"Hydrogel composite scaffolds achieve recruitment and chondrogenesis in cartilage tissue engineering applications","volume":"20","author":"Huang","year":"2022","journal-title":"J Nanobiotechnology"},{"key":"2025042616415764100_rbaf019-B41","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.actbio.2017.06.012","article-title":"Improvement of endothelial progenitor outgrowth cell (EPOC)-mediated vascularization in gelatin-based hydrogels through pore size manipulation","volume":"58","author":"Fu","year":"2017","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B42","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.cell.2006.06.044","article-title":"Matrix elasticity directs stem cell lineage specification","volume":"126","author":"Engler","year":"2006","journal-title":"Cell"},{"key":"2025042616415764100_rbaf019-B43","doi-asserted-by":"crossref","first-page":"rbac100","DOI":"10.1093\/rb\/rbac100","article-title":"Paxillin tunes the relationship between cell-matrix and cell-cell adhesions to regulate stiffness-dependent dentinogenesis","volume":"10","author":"Bai","year":"2023","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B44","doi-asserted-by":"crossref","first-page":"27971","DOI":"10.1021\/acsami.0c05024","article-title":"Effects of microstripe geometry on guided cell migration","volume":"12","author":"Yao","year":"2020","journal-title":"ACS Appl Mater Interfaces"},{"key":"2025042616415764100_rbaf019-B45","doi-asserted-by":"crossref","first-page":"111789","DOI":"10.1016\/j.msec.2020.111789","article-title":"Customized additive manufacturing of porous Ti6Al4V scaffold with micro-topological structures to regulate cell behavior in bone tissue engineering","volume":"120","author":"Lei","year":"2021","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"key":"2025042616415764100_rbaf019-B46","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1007\/s42765-022-00135-w","article-title":"3D printed gelatin scaffold with improved shape fidelity and cytocompatibility by using Antheraea pernyi silk fibroin nanofibers","volume":"4","author":"Zou","year":"2022","journal-title":"Adv Fiber Mater"},{"key":"2025042616415764100_rbaf019-B47","doi-asserted-by":"crossref","first-page":"rbad001","DOI":"10.1093\/rb\/rbad001","article-title":"Fiber diameters and parallel patterns: proliferation and osteogenesis of stem cells","volume":"10","author":"Gu","year":"2023","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B48","doi-asserted-by":"crossref","first-page":"rbad056","DOI":"10.1093\/rb\/rbad056","article-title":"Biaxial stretching of polytetrafluoroethylene in industrial scale to fabricate medical ePTFE membrane with node-fibril microstructure","volume":"10","author":"Wang","year":"2023","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B49","doi-asserted-by":"crossref","first-page":"rbad111","DOI":"10.1093\/rb\/rbad111","article-title":"Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration","volume":"11","author":"He","year":"2024","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B50","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1002\/adma.201301762","article-title":"Cell-material interactions revealed via material techniques of surface patterning","volume":"25","author":"Yao","year":"2013","journal-title":"Adv Mater"},{"key":"2025042616415764100_rbaf019-B51","doi-asserted-by":"crossref","first-page":"9001","DOI":"10.1016\/j.biomaterials.2013.08.013","article-title":"Effects of surface molecular chirality on adhesion and differentiation of stem cells","volume":"34","author":"Yao","year":"2013","journal-title":"Biomaterials"},{"key":"2025042616415764100_rbaf019-B52","doi-asserted-by":"crossref","first-page":"rbac098","DOI":"10.1093\/rb\/rbac098","article-title":"Recent advances in regenerative biomaterials","volume":"9","author":"Cao","year":"2022","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B53","doi-asserted-by":"crossref","first-page":"rbad112","DOI":"10.1093\/rb\/rbad112","article-title":"Effects of serum proteins on corrosion rates and product bioabsorbability of biodegradable metals","volume":"11","author":"Zhang","year":"2024","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B54","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.biomaterials.2018.04.021","article-title":"Degradation rate affords a dynamic cue to regulate stem cells beyond varied matrix stiffness","volume":"178","author":"Peng","year":"2018","journal-title":"Biomaterials"},{"key":"2025042616415764100_rbaf019-B55","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/nmat3586","article-title":"Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels","volume":"12","author":"Khetan","year":"2013","journal-title":"Nature Materials"},{"key":"2025042616415764100_rbaf019-B56","doi-asserted-by":"crossref","first-page":"3514","DOI":"10.1038\/s41467-021-23120-0","article-title":"Enhanced mechanosensing of cells in synthetic 3D matrix with controlled biophysical dynamics","volume":"12","author":"Yang","year":"2021","journal-title":"Nat Commun"},{"key":"2025042616415764100_rbaf019-B57","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1360\/TB-2020-1069","article-title":"How cells sense and respond to the mechanical properties of the microenvironment","volume":"66","author":"Sun","year":"2021","journal-title":"Chin Sci Bull"},{"key":"2025042616415764100_rbaf019-B58","first-page":"1375","article-title":"Adaptable hydrogel with reversible linkages for regenerative medicine: dynamic mechanical microenvironment for cells","volume":"6","author":"Tong","year":"2021","journal-title":"Bioact Mater"},{"key":"2025042616415764100_rbaf019-B59","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1038\/s41586-020-2612-2","article-title":"Effects of extracellular matrix viscoelasticity on cellular behaviour","volume":"584","author":"Chaudhuri","year":"2020","journal-title":"Nature"},{"key":"2025042616415764100_rbaf019-B60","doi-asserted-by":"crossref","first-page":"rbad054","DOI":"10.1093\/rb\/rbad054","article-title":"Phosphoserine enhanced Cu-doped bioactive glass dynamic dual-network hydrogel for craniofacial bone defect repair","volume":"10","author":"Liu","year":"2023","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B61","doi-asserted-by":"crossref","first-page":"rbab066","DOI":"10.1093\/rb\/rbab066","article-title":"Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis","volume":"8","author":"Jing","year":"2021","journal-title":"Regen Biomater"},{"key":"2025042616415764100_rbaf019-B62","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.actbio.2019.01.021","article-title":"Molecular and macro-scale analysis of enzyme-crosslinked silk hydrogels for rational biomaterial design","volume":"86","author":"McGill","year":"2019","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B63","doi-asserted-by":"crossref","first-page":"119720","DOI":"10.1016\/j.biomaterials.2019.119720","article-title":"Enzymatically crosslinked silk and silk-gelatin hydrogels with tunable gelation kinetics, mechanical properties and bioactivity for cell culture and encapsulation","volume":"232","author":"Hasturk","year":"2020","journal-title":"Biomaterials"},{"key":"2025042616415764100_rbaf019-B64","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1039\/c3tb21148g","article-title":"Nanoconfined crystallites toughen artificial silk","volume":"2","author":"Pan","year":"2014","journal-title":"J Mater Chem B"},{"key":"2025042616415764100_rbaf019-B65","doi-asserted-by":"crossref","first-page":"3640","DOI":"10.1039\/C7TB00485K","article-title":"Robust silk fibroin\/bacterial cellulose nanoribbon composite scaffolds with radial lamellae and intercalation structure for bone regeneration","volume":"5","author":"Chen","year":"2017","journal-title":"J Mater Chem B"},{"key":"2025042616415764100_rbaf019-B66","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.actbio.2017.09.020","article-title":"Molecular and macro-scale analysis of enzyme-crosslinked silk hydrogels for rational biomaterial design","volume":"63","author":"McGill","year":"2017","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B67","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1021\/acs.biomac.6b01086","article-title":"Tyrosine templating in the self-assembly and crystallization of silk fibroin","volume":"17","author":"Partlow","year":"2016","journal-title":"Biomacromolecules"},{"key":"2025042616415764100_rbaf019-B68","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1111\/jphp.13262","article-title":"Pyoluteorin induces cell cycle arrest and apoptosis in human triple-negative breast cancer cells MDA-MB-231","volume":"72","author":"Ding","year":"2020","journal-title":"J Pharm Pharmacol"},{"key":"2025042616415764100_rbaf019-B69","doi-asserted-by":"crossref","first-page":"4615","DOI":"10.1002\/adfm.201400526","article-title":"Highly tunable elastomeric silk biomaterials","volume":"24","author":"Partlow","year":"2014","journal-title":"Adv Funct Mater"},{"key":"2025042616415764100_rbaf019-B70","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/nprot.2015.024","article-title":"Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy","volume":"10","author":"Yang","year":"2015","journal-title":"Nat Protoc"},{"key":"2025042616415764100_rbaf019-B71","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.actbio.2018.03.058","article-title":"A robust spectroscopic method for the determination of protein conformational composition\u2014application to the annealing of silk","volume":"73","author":"Belton","year":"2018","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B72","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1039\/b719557e","article-title":"An injectable enzymatically crosslinked hyaluronic acid-tyramine hydrogel system with independent tuning of mechanical strength and gelation rate","volume":"4","author":"Lee","year":"2008","journal-title":"Soft Matter"},{"key":"2025042616415764100_rbaf019-B73","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1002\/adma.201500280","article-title":"From soft self-healing gels to stiff films in suckerin-based materials through modulation of crosslink density and \u03b2-sheet content","volume":"27","author":"Ding","year":"2015","journal-title":"Adv Mater"},{"key":"2025042616415764100_rbaf019-B74","doi-asserted-by":"crossref","first-page":"025006","DOI":"10.1088\/1748-605X\/ac494b","article-title":"Macroporous methacrylated hyaluronic acid hydrogel with different pore sizes for in vitro and in vivo evaluation of vascularization","volume":"17","author":"Lu","year":"2022","journal-title":"Biomed Mater"},{"key":"2025042616415764100_rbaf019-B75","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1080\/09205063.2012.696310","article-title":"Modulation of the proliferation and matrix synthesis of chondrocytes by dynamic compression on genipin-crosslinked chitosan\/collagen scaffolds","volume":"24","author":"Wang","year":"2013","journal-title":"J Biomater Sci Polym Ed"},{"key":"2025042616415764100_rbaf019-B76","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/adfm.201001519","article-title":"Dynamic electromechanical hydrogel matrices for stem cell culture","volume":"21","author":"Lim","year":"2011","journal-title":"Adv Funct Mater"},{"key":"2025042616415764100_rbaf019-B77","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1021\/acs.biomac.4c01676","article-title":"Effects of silk fibroin hydrogel degradation on the proliferation and chondrogenesis of encapsulated stem cells","volume":"26","author":"Zhu","year":"2025","journal-title":"Biomacromolecules"},{"key":"2025042616415764100_rbaf019-B78","doi-asserted-by":"crossref","first-page":"eabe4501","DOI":"10.1126\/sciadv.abe4501","article-title":"Injury-mediated stiffening persistently activates muscle stem cells through YAP and TAZ mechanotransduction","volume":"7","author":"Silver","year":"2021","journal-title":"Sci Adv"},{"key":"2025042616415764100_rbaf019-B79","doi-asserted-by":"crossref","first-page":"31037","DOI":"10.1038\/srep31037","article-title":"Tumor growth suppression induced by biomimetic silk fibroin hydrogels","volume":"6","author":"Yan","year":"2016","journal-title":"Sci Rep"},{"key":"2025042616415764100_rbaf019-B80","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1002\/(SICI)1521-1878(200002)22:2<138::AID-BIES5>3.0.CO;2-4","article-title":"All for one and one for all: condensations and the initiation of skeletal development","volume":"22","author":"Hall","year":"2000","journal-title":"Bioessays"},{"key":"2025042616415764100_rbaf019-B81","first-page":"4420","article-title":"Fibronectin matrix assembly is essential for cell condensation during chondrogenesis","volume":"127","author":"Singh","year":"2014","journal-title":"J Cell Sci"},{"key":"2025042616415764100_rbaf019-B82","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/nmat4489","article-title":"Hydrogels with tunable stress relaxation regulate stem cell fate and activity","volume":"15","author":"Chaudhuri","year":"2016","journal-title":"Nat Mater"},{"key":"2025042616415764100_rbaf019-B83","doi-asserted-by":"crossref","first-page":"260","DOI":"10.12965\/jer.1632640.320","article-title":"Matrix metalloproteinase and tissue inhibitor of metalloproteinase responses to muscle damage after eccentric exercise","volume":"12","author":"Kim","year":"2016","journal-title":"J Exerc Rehabil"},{"key":"2025042616415764100_rbaf019-B84","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.actbio.2019.04.054","article-title":"Influence of hydrogel network microstructures on mesenchymal stem cell chondrogenesis in vitro and in vivo","volume":"91","author":"Yang","year":"2019","journal-title":"Acta Biomater"},{"key":"2025042616415764100_rbaf019-B85","doi-asserted-by":"crossref","first-page":"3701","DOI":"10.1002\/jcp.24078","article-title":"Recapitulation of mesenchymal condensation enhances in vitro chondrogenesis of human mesenchymal stem cells","volume":"227","author":"Ghone","year":"2012","journal-title":"J Cell Physiol"}],"container-title":["Regenerative Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/rb\/advance-article-pdf\/doi\/10.1093\/rb\/rbaf019\/62493584\/rbaf019.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/rb\/article-pdf\/doi\/10.1093\/rb\/rbaf019\/62493584\/rbaf019.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/rb\/article-pdf\/doi\/10.1093\/rb\/rbaf019\/62493584\/rbaf019.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T20:42:13Z","timestamp":1745700133000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/rb\/article\/doi\/10.1093\/rb\/rbaf019\/8088247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":85,"URL":"https:\/\/doi.org\/10.1093\/rb\/rbaf019","relation":{},"ISSN":["2056-3426"],"issn-type":[{"value":"2056-3426","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025]]},"published":{"date-parts":[[2025]]},"article-number":"rbaf019"}}