{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T14:20:40Z","timestamp":1782310840226,"version":"3.54.5"},"reference-count":44,"publisher":"Scientific Societies","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plant Disease"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p> Incidence and nematode population densities of plant-parasitic nematodes were determined in 64 samples of soil and grapevine roots collected from commercial vineyards in southern Spain between October 2003 and May 2005. In addition, a histopathological study was done of root-stock roots naturally infected by root-knot nematodes (Meloidogyne spp.). Nematodes infecting the rootstocks were identified according to conventional procedures, and the Meloidogyne spp. were furthermore identified by sequence characterized amplified region-polymerase chain reaction (SCAR-PCR) and isozyme esterase analyses. The most important plant-parasitic nematodes detected, in order of decreasing frequency of total soil infestation and root infection (percentage of samples), were Mesocriconema xenoplax (34.4%), Meloidogyne incognita (26.6%), Meloidogyne javanica (14.1%), Xiphinema index (12.5%), Xiphinema italiae (10.9%), Pratylenchus vulnus (6.3%), and Meloidogyne arenaria (1.6%). No disease symptoms were observed on aboveground plant parts of the infected grapevines, except for plants in some fields where soil was infested with the virus-vector nematodes X. index and X. italiae. Those grapevines showed a yellow mosaic pattern in leaves early in the growing season and the internode shortening characteristic of infections by Grapevine fanleaf virus. Rootstocks infected by root-knot nematodes (Meloidogyne spp.) showed distorted feeder roots and large- to moderate-sized root galls, present either singly or in clusters. Histopathology of galled roots showed a typical susceptible response to infection by root-knot nematodes: cellular alterations were induced in the cortex, endodermis, pericycle, and vascular system, including giant-cell formation and severe distortion of vascular tissues. Most Meloidogyne egg masses ocurred on the surface of the galled root tissues, a position that could facilitate dispersion of the nematode eggs and juveniles and the occurrence of secondary infections. Some of the grapevine rootstocks surveyed in this study (Paulsen 1103, Richter 110, Rupestris du Lot, and SO4) had previously been reported to be resistant to Meloidogyne spp.; however, the population densities of these nematodes found in soil and roots sampled in the present study, as well as the compatible host-parasite relationship revealed by histopathology, indicate a susceptible response to Meloidogyne spp. from southern Spain. <\/jats:p>","DOI":"10.1094\/pdis-91-9-1147","type":"journal-article","created":{"date-parts":[[2007,8,9]],"date-time":"2007-08-09T18:25:05Z","timestamp":1186683905000},"page":"1147-1154","source":"Crossref","is-referenced-by-count":31,"title":["Plant-Parasitic Nematodes Infecting Grapevine in Southern Spain and Susceptible Reaction to Root-Knot Nematodes of Rootstocks Reported as Moderately Resistant"],"prefix":"10.1094","volume":"91","author":[{"given":"Daniel","family":"T\u00e9liz","sequence":"first","affiliation":[{"name":"Instituto de Fitosanidad, Colegio de Postgraduados,56230 Montecillo, Texcoco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca B.","family":"Landa","sequence":"additional","affiliation":[{"name":"Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Apdo. 4084, 14080 C\u00f3rdoba, Spain, and Escuela T\u00e9cnica Superior de Ingenieros Agr\u00f3nomos y de Montes (ETSIAM), Universidad de C\u00f3rdoba (UCO), Edificio C4- \u201cCelestino Mutis\u201d, Carretera de Madrid Km 396, Campus de Rabanales, 14071 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hava F.","family":"Rapoport","sequence":"additional","affiliation":[{"name":"IAS-CSIC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando P\u00e9rez","family":"Camacho","sequence":"additional","affiliation":[{"name":"ETSIAM-UCO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael M.","family":"Jim\u00e9nez-D\u00edaz","sequence":"additional","affiliation":[{"name":"ETSIAM-UCO and IAS-CSIC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Castillo","sequence":"additional","affiliation":[{"name":"IAS-CSIC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1665","reference":[{"key":"p_1","first-page":"319","volume":"65","author":"Aballay E.","year":"2005","journal-title":"Agric. T\u00e9cnica"},{"key":"p_2","first-page":"28","volume":"34","author":"Anwar S. A.","year":"2002","journal-title":"J. Nematol."},{"key":"p_3","first-page":"265","volume":"32","author":"Anwar S. A.","year":"2000","journal-title":"J. Nematol."},{"key":"p_4","first-page":"276","volume":"21","author":"Anwar S. A.","year":"1989","journal-title":"J. Nematol."},{"key":"p_5","first-page":"727","volume":"26","author":"Bird G.","year":"1994","journal-title":"J. Nematol."},{"key":"p_6","unstructured":"Brown, D. J., Dalmasso, A., and Trudgill, D. L. 1993. Nematode pests of soft fruits and vines. Pages 427-462 in: Plant-Parasitic Nematodes in Temperate Agriculture. K. Evans, D. L. Trudgill, and J. M. Webster, eds. CAB International, Wallingford, UK."},{"key":"p_7","doi-asserted-by":"publisher","DOI":"10.1163\/156854104323072982"},{"key":"p_8","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2003.93.9.1093"},{"key":"p_9","unstructured":"Coolen, W. A. 1979. Methods for extraction of Meloidogyne spp. and other nematodes from roots and soil. Pages 317-329 in: Root-Knot Nematodes (Meloidogyne species). Systematics, Biology, and Control. F. Lamberti and C. E. Taylor, eds. Academic Press, New York."},{"key":"p_10","first-page":"107","volume":"14","author":"Doucet M.","year":"1984","journal-title":"Rev. Invest. Agropec. INTA"},{"key":"p_11","doi-asserted-by":"publisher","DOI":"10.1071\/EA9890129"},{"key":"p_12","unstructured":"Esbenshade, P. R., and Triantaphyllou, A. C. 1985. Identification of Meloidogyne species employing enzyme phenotypes as differentiating characters. Pages 135-140 in: An Advanced Treatise on Meloidogyne. Vol. 2. Methodology. K. R. Barker, C. C. Carter, and J. N. Sasser, eds. North Carolina State University Graphics, Raleigh."},{"key":"p_13","unstructured":"FAO, 2005. Faostat Database Collections. FAO. Online publication."},{"key":"p_14","first-page":"295","volume":"7","author":"Ferris H.","year":"1975","journal-title":"J. Nematol."},{"key":"p_15","first-page":"71","volume":"10","author":"Hafez S. L.","year":"2000","journal-title":"Int. J. Nematol."},{"key":"p_16","unstructured":"Hartman, K. M., and Sasser, J. N. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. Pages 69-77 in: An Advanced Treatise on Meloidogyne. Vol. 2. Methodology. K. R. Barker, C. C. Carter, and J. N. Sasser, eds. North Carolina State University Graphics, Raleigh."},{"key":"p_17","first-page":"1025","volume":"57","author":"Hussey R. S.","year":"1973","journal-title":"Plant Dis. Rep."},{"key":"p_18","doi-asserted-by":"crossref","unstructured":"Hussey, R. S., and Williamson, V. M. 1997. Physiological and molecular aspects of nematode parasitism. Pages 87-108 in: Plant and Nematode Interactions. K. R. Barker, G. A. Pederson, and G. L. Windham, eds. American Society of Agronomy, Madison, WI.","DOI":"10.2134\/agronmonogr36.c5"},{"key":"p_19","unstructured":"Jensen, W. A. 1962. Botanical Histochemistry. W.H. Freeman and Co., San Francisco, CA."},{"key":"p_20","first-page":"62","volume":"27","author":"Jenser G.","year":"1991","journal-title":"Novenyvedelem"},{"key":"p_21","unstructured":"Jepson, S. B. 1987. Identification of root-knot nematodes. CAB International, Wallingford, UK."},{"key":"p_22","first-page":"283","volume":"25","author":"Kleynhans K. P. N.","year":"1993","journal-title":"Phytophylactica"},{"key":"p_23","first-page":"1","volume":"18","author":"Lamberti F.","year":"1990","journal-title":"Nematol. Mediterr."},{"key":"p_24","unstructured":"McKenry, M. V. 1987. Control strategies in high-value crops. Pages 329-349 in: Principles and Practice of Nematode Control in Crops. R. H. Brown and B. R. Kerry, eds. Academic Press, Orlando, FL."},{"key":"p_25","first-page":"312","volume":"38","author":"McKenry M. V.","year":"2006","journal-title":"J. Nematol."},{"key":"p_26","first-page":"414","volume":"31","author":"Mor M.","year":"2003","journal-title":"Phytoparasitica"},{"key":"p_27","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS.2002.86.10.1075"},{"key":"p_28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0238.1999.tb00295.x"},{"key":"p_29","unstructured":"Pe\u00f1a Santiago, R., Castillo, P., Escuer, M., Guerrero, P., Talavera, M., and Vieira, P. 2004. Tylenchid species (Nematoda, Tylenchida) recorded in the Iberian Peninsula and the Balearic Islands: A Compendium. Collection \"Monographic Papers on Nematology\", Servicio de Publicaciones, Universidad de Ja\u00e9n, Ja\u00e9n, Spain."},{"key":"p_30","first-page":"193","volume":"36","author":"Pinkerton J. N.","year":"2004","journal-title":"J. Nematol."},{"key":"p_31","first-page":"391","volume":"9","author":"Pinochet J.","year":"1986","journal-title":"Rev. N\u00e9matol."},{"key":"p_32","doi-asserted-by":"publisher","DOI":"10.1071\/AP01046"},{"key":"p_33","unstructured":"Raski, D. J. 1988. Parasites of grapes. Pages 55-59 in: Compendium of Grape Diseases. R. C. Pearson and A. C. Goheen, eds. American Phytopathological Society, St. Paul, MN."},{"key":"p_34","unstructured":"Raski, D. J., Hart, W. H., and Kasimatis, A. N. 1973. Nematodes and their control in vineyards. Calif. Agric. Exp. Stn. Circ. 533."},{"key":"p_35","unstructured":"Salmer\u00f3n Parra, T., Andreu L\u00f3pez, M., and Tobar Jim\u00e9nez, A. 1986. Observaciones sobre el \"amerinado\" de los vi\u00f1edos del condado de Huelva. Bol. Serv. Plagas 12:143-146."},{"key":"p_36","first-page":"215","volume":"9","author":"Santo G. S.","year":"1977","journal-title":"J. Nematol."},{"key":"p_37","doi-asserted-by":"publisher","DOI":"10.1163\/187529262X00981"},{"key":"p_38","doi-asserted-by":"publisher","DOI":"10.1071\/EA9840277"},{"key":"p_39","doi-asserted-by":"publisher","DOI":"10.1071\/APP9920104"},{"key":"p_40","first-page":"399","volume":"20","author":"Verdejo Lucas S.","year":"1997","journal-title":"Fund. Appl. Nematol."},{"key":"p_41","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2005.01244.x"},{"key":"p_42","doi-asserted-by":"publisher","DOI":"10.1094\/PD-78-1055"},{"key":"p_43","doi-asserted-by":"publisher","DOI":"10.17660\/ActaHortic.1995.388.4"},{"key":"p_44","doi-asserted-by":"publisher","DOI":"10.1163\/156854100750112798"}],"container-title":["Plant Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/apsjournals.apsnet.org\/doi\/pdf\/10.1094\/PDIS-91-9-1147","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T22:15:50Z","timestamp":1640816150000},"score":1,"resource":{"primary":{"URL":"https:\/\/apsjournals.apsnet.org\/doi\/10.1094\/PDIS-91-9-1147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":44,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1094\/PDIS-91-9-1147"],"URL":"https:\/\/doi.org\/10.1094\/pdis-91-9-1147","relation":{},"ISSN":["0191-2917","1943-7692"],"issn-type":[{"value":"0191-2917","type":"print"},{"value":"1943-7692","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}