{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:05:25Z","timestamp":1762297525825},"reference-count":62,"publisher":"Scientific Societies","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plant Disease"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>Polymerase chain reaction (PCR) primers (BXW-1 and BXW-3) for conventional PCR were developed from conserved sequences in the hrpB operon of the hrp gene cluster from Xanthomonas campestris pv. musacearum, the causative agent of banana Xanthomonas wilt (BXW). All 50 strains of X. campestris pv. musacearum, isolated from Uganda, Rwanda, and Tanzania, produced a 214-bp amplicon when whole cells, bacterial ooze from infected tissue, and genomic DNA purified from bacterial ooze or infected tissue were used as template. The BXW primers also detected strains of X. axonopodis pv. vasculorum isolated from sugarcane and maize and strains of X. vasicola pv. holcicola isolated from sorghum. All of the strains of X. campestris pv. musacearum were clonal when compared using enterobacterial repetitive intergenic consensus PCR.<\/jats:p>","DOI":"10.1094\/pdis-94-1-0109","type":"journal-article","created":{"date-parts":[[2009,12,4]],"date-time":"2009-12-04T18:10:23Z","timestamp":1259950223000},"page":"109-114","source":"Crossref","is-referenced-by-count":21,"title":["A Polymerase Chain Reaction Assay for the Detection of<i>Xanthomonas campestris<\/i>pv.<i>musacearum<\/i>in Banana"],"prefix":"10.1094","volume":"94","author":[{"given":"Melanie L.","family":"Lewis Ivey","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffrey","family":"Tusiime","sequence":"additional","affiliation":[{"name":"Makerere University, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally A.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1665","reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.18.5655-5662.1997"},{"key":"p_2","first-page":"179","volume":"6","author":"Aritua V.","year":"2008","journal-title":"Afr. J. Biotechnol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1111\/j.1365-3059.2007.01687.x","volume":"57","author":"Aritua V.","year":"2008","journal-title":"Plant Pathol."},{"key":"p_4","unstructured":"Bafana, B. 2008. Trade-East Africa: Going Bananas to Fight Poverty and Hunger. IPS Inter Press Service News Agency (IPS). http:\/\/ ipsnews.net\/news.asp?idnews=44210. Accessed October 2008."},{"key":"p_5","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.11.3522-3526.1992"},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2005.01186.x"},{"key":"p_7","first-page":"953","volume":"6","author":"Biruma M.","year":"2007","journal-title":"Afr. J. Biotechnol."},{"key":"p_8","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-95-0708"},{"key":"p_9","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.3.1257-1264.2002"},{"key":"p_10","doi-asserted-by":"publisher","DOI":"10.1094\/PD-90-0451"},{"key":"p_11","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.56.6.1743-1749.1990"},{"key":"p_12","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1128\/aem.58.7.2180-2187.1992","volume":"58","author":"de Bruijn F. J.","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-80-618"},{"key":"p_14","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-85-462"},{"key":"p_15","first-page":"153","volume":"59","author":"Dye D. W.","year":"1980","journal-title":"Rev. Plant Pathol."},{"key":"p_16","unstructured":"Engels, B. 2005. Amplify 3.1X. University of Wisconsin. http:\/\/engels.genetics.wisc.edu\/ amplify\/. Accessed September 2006."},{"key":"p_17","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.3.186"},{"key":"p_18","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.3.175"},{"key":"p_19","unstructured":"Food and Agriculture Organization of The United Nations. 2005. Banana Statistics, 2005. http:\/\/faostat.fao.org\/site\/339\/default.aspx. Accessed January 2005."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1128\/aem.59.4.1143-1148.1993","volume":"59","author":"Hartung J. S.","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"p_21","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-328"},{"key":"p_22","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2002.92.10.1077"},{"key":"p_23","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"p_24","doi-asserted-by":"publisher","DOI":"10.1094\/PD-79-0917"},{"key":"p_25","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.60.4.1068-1077.1994"},{"key":"p_26","unstructured":"Lelliot, R. A., and Stead, D. E. 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Blackwell Scientific Publication, Oxford."},{"key":"p_27","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-85-837"},{"key":"p_28","doi-asserted-by":"publisher","DOI":"10.1128\/jb.168.2.512-522.1986"},{"key":"p_29","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2286-2295.1994"},{"key":"p_30","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2004.94.1.61"},{"key":"p_31","unstructured":"Mbaka, J., Ndungu, V., and Mwangi, M. 2007. Outbreak of Xanthomonas wilt (Xanthomonas campestris pv. musacearum) on banana in Kenya. Pages 58-59 in: Program and Abstracts of ISHS\/ProMusa Symposium: Recent Advances in Banana Crop Protection for Sustainable Production and Improved Livelihoods. Greenway Woods Resort, White River, South Africa."},{"key":"p_32","unstructured":"Mgenzi, S. R. B, Eden-Green S., and Peacock, J. 2006. Overview of banana Xanthomonas wilt in Tanzania. In: Proc. 4th Int. Bacterial Wilt Symp. Central Science Laboratory, York, UK."},{"key":"p_33","first-page":"43","volume":"13","author":"Ndungo V.","year":"2005","journal-title":"Infomusa"},{"key":"p_34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2005.01258.x"},{"key":"p_35","doi-asserted-by":"publisher","DOI":"10.1023\/B:EJPP.0000019797.27952.1d"},{"key":"p_36","first-page":"19","volume":"5","author":"Opina N","year":"1997","journal-title":"Asia Pac. J. Mol. Biol. Biotechnol."},{"key":"p_37","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2004.09.002"},{"key":"p_38","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65825-0"},{"key":"p_39","doi-asserted-by":"publisher","DOI":"10.1007\/BF00294703"},{"key":"p_40","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(00)80021-1"},{"key":"p_41","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-83-965"},{"key":"p_42","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2007.01640.x"},{"key":"p_43","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80178-5"},{"key":"p_44","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.7.4249-4255.2003"},{"key":"p_45","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2003.93.1.64"},{"key":"p_46","unstructured":"Schaad, N. W., and Stall, R. E. 1988. Xanthomonas. Pages 81-94 in: Laboratory Guide for Identification of Plant Pathogenic Bacteria, 2nd ed. N. W. Schaad, ed. APS Press, St. Paul, MN."},{"key":"p_47","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.12.7248-7256.2003"},{"key":"p_48","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-7-1587"},{"key":"p_49","unstructured":"Smith, J. J., Jones, D. R., Karamura, E., Blomme, G., and Turyagyenda, F. L. 2008. An Analysis of the Risk from Xanthomonas campestris pv. musacearum to Banana Cultivation in Eastern, Central and Southern Africa. Biodiversity International, Montpellier, France."},{"key":"p_50","first-page":"581","volume":"25","author":"Sousa S. M.","year":"1997","journal-title":"Technol."},{"key":"p_51","unstructured":"Stall., R. E., and Minsavage, G. V. 1990. The use of hrp genes to identify opportunistic xanthomonads. Pages 369-374 in: Proc. 7th Int. Conf. Plant Pathol. Z. Klement, ed. Budapest."},{"key":"p_52","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-1-10"},{"key":"p_53","unstructured":"Thwaites, R., Eden-Green, S. J., and Black, R. 2000. Diseases caused by bacteria. Pages 213-240 in: Diseases of Banana: Abaca and Enset, D. R. Jones, ed. CAB International, Wallingford, UK."},{"key":"p_54","first-page":"6","volume":"12","author":"Tushemereirwe W.","year":"2003","journal-title":"Infomusa"},{"key":"p_55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2004.01090.x"},{"key":"p_56","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-4-132"},{"key":"p_57","first-page":"111","volume":"58","author":"Yirgou D.","year":"1968","journal-title":"Phytopathology"},{"key":"p_58","doi-asserted-by":"publisher","DOI":"10.1080\/00128325.1974.11662720"},{"key":"p_59","unstructured":"Young, J. M., Bull, C. T., De Boer, S. H., Firrao, G., Gardan, L., Saddler, G. E., Stead, D. E., and Takikawa, Y. 2004. Names of Plant Pathogenic Bacteria Since 1995. Report of the Taxonomy of Bacterial Plant Pathogens Committee of the International Society of Plant Pathology. http:\/\/www.isppweb.org\/names_ bacterial_new2004.asp. Accessed March 2004."},{"key":"p_60","doi-asserted-by":"publisher","DOI":"10.1080\/00288233.1978.10427397"},{"key":"p_61","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-007-9115-y"},{"key":"p_62","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-004-2734-7"}],"container-title":["Plant Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/apsjournals.apsnet.org\/doi\/pdf\/10.1094\/PDIS-94-1-0109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T00:42:47Z","timestamp":1710722567000},"score":1,"resource":{"primary":{"URL":"https:\/\/apsjournals.apsnet.org\/doi\/10.1094\/PDIS-94-1-0109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1094\/PDIS-94-1-0109"],"URL":"https:\/\/doi.org\/10.1094\/pdis-94-1-0109","relation":{},"ISSN":["0191-2917","1943-7692"],"issn-type":[{"value":"0191-2917","type":"print"},{"value":"1943-7692","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]}}}