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It also showed lower tolerance to acidic conditions, a reduced production of siderophores, and lower motility and biofilm formation. The simultaneous deletion of emrA and emrR genes restored the mentioned traits to the wild-type phenotype, except for survival under heat shock, which was lower than that displayed by the wild-type strain. Furthermore, the \u0394emrR mutant as well as the double \u0394emrAR mutant was impaired in symbiosis with Medicago sativa; it formed fewer nodules and competed poorly with the wild-type strain for nodule colonization. Expression profiling of the \u0394emrR mutant showed decreased expression of genes involved in Nod-factor and rhizobactin biosynthesis and in stress responses. Expression of genes directing the biosynthesis of succinoglycan and other polysaccharides were increased. EmrR may therefore be involved in a regulatory network targeting membrane and cell wall modifications in preparation for colonization of root hairs during symbiosis. <\/jats:p>","DOI":"10.1094\/mpmi-09-13-0284-r","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T21:06:29Z","timestamp":1393967189000},"page":"388-399","source":"Crossref","is-referenced-by-count":21,"title":["The <i>Sinorhizobium meliloti<\/i> EmrR Regulator Is Required for Efficient Colonization of <i>Medicago sativa<\/i> Root Nodules"],"prefix":"10.1094","volume":"27","author":[{"given":"M\u00e1rio R.","family":"Santos","sequence":"first","affiliation":[]},{"given":"Andreia T.","family":"Marques","sequence":"additional","affiliation":[]},{"given":"J\u00f6rg D.","family":"Becker","sequence":"additional","affiliation":[]},{"given":"Leonilde M.","family":"Moreira","sequence":"additional","affiliation":[]}],"member":"1665","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"5280","DOI":"10.1128\/JB.181.17.5280-5287.1999","volume":"181","author":"Abola A. 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