{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T16:31:08Z","timestamp":1708533068114},"reference-count":38,"publisher":"Scientific Societies","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytopathology\u00ae"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p> Plant-parasitic nematodes possess a large number of proteins that are secreted in planta, allowing them to be successful parasites of plants. The majority of these proteins are synthesized mainly in the nematode subventral and dorsal glands as well as in other organs. To improve the immunovisualization of these proteins, we adapted a methacrylate embedding method for the localization of proteins inside nematode tissues, and extracellularly when secreted in planta or within plant cells. An important advantage is that the method is applicable for all nematode stages: preparasitic as well as parasitic stages, including large mature females. Herein, the method has been successfully applied for the localization of four nematode secreted proteins, such as Mi-MAP-1, Mi-CBM2-bearing proteins, Mi-PEL3, and Mi-6D4. In addition, we could also localize 14-3-3 proteins, as well as two cytoskeletal proteins, by double-immunolabeling on preparasitic juveniles. Superior preservation of nematode and plant morphology, allowed more accurate protein localization as compared with other methods. Besides excellent epitope preservation, dissolution of methacrylate from tissue sections unmasks target proteins and thereby drastically increases antibody access. <\/jats:p>","DOI":"10.1094\/phyto-02-12-0031-r","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T21:49:41Z","timestamp":1339537781000},"page":"990-996","source":"Crossref","is-referenced-by-count":8,"title":["An Immunocytochemical Procedure for Protein Localization in Various Nematode Life Stages Combined with Plant Tissues Using Methylacrylate-Embedded Specimens"],"prefix":"10.1094","volume":"102","author":[{"given":"Paulo","family":"Vieira","sequence":"first","affiliation":[]},{"given":"Mohamed Youssef","family":"Banora","sequence":"additional","affiliation":[]},{"given":"Philippe","family":"Castagnone-Sereno","sequence":"additional","affiliation":[]},{"given":"Marie-No\u00eblle","family":"Rosso","sequence":"additional","affiliation":[]},{"given":"Gilbert","family":"Engler","sequence":"additional","affiliation":[]},{"given":"Janice","family":"de Almeida Engler","sequence":"additional","affiliation":[]}],"member":"1665","reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002343"},{"key":"p_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00195665"},{"key":"p_3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000192"},{"key":"p_4","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(83)80038-1"},{"key":"p_5","unstructured":"Coolen, W. A. 1979. Methods for the extraction of Meloidogyne spp. species and other nematodes\u2028from roots and soil. Pages 317-329 in: Root-Knot Nematodes (Meloidogyne Species) Systematics, Biology and Control. F. Lamberti and C. E. Taylor, eds. Academic Press, London & New York."},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000067640"},{"key":"p_7","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-82-1244"},{"key":"p_8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.38.1.365"},{"key":"p_9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02019.x"},{"key":"p_10","doi-asserted-by":"publisher","DOI":"10.1007\/BF02669275"},{"key":"p_11","doi-asserted-by":"crossref","unstructured":"De Almeida Engler, J., Van Montagu, M., and Engler, G. 1998. Whole-Mount in situ hybridization in plants. Pages 373-384 in: Arabidopsis Protocols. Methods in Molecular Biology. J. M. Mart\u00ednez-Zapater and J. Salinas, eds. Humana Press, Totowa, NJ.","DOI":"10.1385\/0-89603-391-0:373"},{"key":"p_12","first-page":"309","volume":"30","author":"De Boer J. M.","year":"1998","journal-title":"J. Nematol."},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2002.15.6.549"},{"key":"p_14","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500215"},{"key":"p_15","doi-asserted-by":"publisher","DOI":"10.1016\/S0309-1651(89)80015-3"},{"key":"p_16","doi-asserted-by":"publisher","DOI":"10.1177\/35.3.3546483"},{"key":"p_17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.01.006"},{"key":"p_18","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2003.16.5.376"},{"key":"p_19","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-80-709"},{"key":"p_20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2012.04164.x"},{"key":"p_21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2004.02.008"},{"key":"p_22","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-18-1277"},{"key":"p_23","doi-asserted-by":"publisher","DOI":"10.1006\/cbir.1993.1031"},{"key":"p_24","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.06.023"},{"key":"p_25","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9452(01)00458-7"},{"key":"p_26","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-41582005000600010"},{"key":"p_27","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2003.16.12.1077"},{"key":"p_28","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.5.12.1697"},{"key":"p_29","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.039644"},{"key":"p_30","doi-asserted-by":"crossref","unstructured":"Rosso, M., Hussey, R., Davis, E. L., Smant, G., Baum, T., Abad, P., and Mitchum, M. G. 2011. Nematode effector proteins: Targets and functions in plant parasitism. Pages 327-354 in: Effectors in Plant-Microbe Interactions. F. Martin and S. Kamoun, eds. John Wiley & Sons, Inc., New York.","DOI":"10.1002\/9781119949138.ch13"},{"key":"p_31","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1999.12.7.585"},{"key":"p_32","unstructured":"Ruzin, S. E. 1999. Butyl\/methyl methacrylate embedding. Page 4 in: Plant Microtechnique and Microscopy. S. E. Ruzin, ed. Oxford University Press, New York."},{"key":"p_33","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2001.14.1.72"},{"key":"p_34","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-294-6_14"},{"key":"p_35","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000068165"},{"key":"p_36","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953695"},{"key":"p_37","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq352"},{"key":"p_38","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600203"}],"container-title":["Phytopathology\u00ae"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/apsjournals.apsnet.org\/doi\/pdf\/10.1094\/PHYTO-02-12-0031-R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T17:38:38Z","timestamp":1640799518000},"score":1,"resource":{"primary":{"URL":"https:\/\/apsjournals.apsnet.org\/doi\/10.1094\/PHYTO-02-12-0031-R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1094\/PHYTO-02-12-0031-R"],"URL":"https:\/\/doi.org\/10.1094\/phyto-02-12-0031-r","relation":{},"ISSN":["0031-949X","1943-7684"],"issn-type":[{"value":"0031-949X","type":"print"},{"value":"1943-7684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10]]}}}