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As a result of this first contact, the host triggers a defense response that involves the modulation of extra- and intracellular proteins. In grapevine\u2212pathogen interactions, little is known about the trafficking between extra- and intracellular spaces. Grapevine is an economically important crop that relies on heavy fungicide use to control several diseases, and a deeper knowledge on the activation of its immune response is crucial to define new control strategies. In this study, we focused on the first 6 h postinoculation with Plasmopara viticola to evaluate grapevine proteome modulation in the apoplast. The in planta P. viticola proteome was also assessed to enable a deeper understanding of plant\u2212pathogen communication. Our results showed that several plant mechanisms are triggered in the tolerant grapevine cultivar Regent after inoculation, such as oomycete recognition, plant cell wall modifications, reactive oxygen species signaling, and secretion of proteins to disrupt oomycete structures. On the other hand, P. viticola proteins related to development and virulence were the most predominant. This pioneer study highlights the early dynamics of cellular communication in grapevine defense that leads to the successful establishment of an incompatible interaction. <\/jats:p>","DOI":"10.1094\/phyto-09-22-0340-fi","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T15:52:05Z","timestamp":1667317925000},"page":"893-903","source":"Crossref","is-referenced-by-count":8,"title":["Deep into the Apoplast: Grapevine and <i>Plasmopara viticola<\/i> Proteomes Reveal the Secret Beneath Host and Pathogen Communication at 6 h After Contact"],"prefix":"10.1094","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7795-2531","authenticated-orcid":false,"given":"Joana","family":"Figueiredo","sequence":"first","affiliation":[{"name":"Grapevine Pathogen Systems Lab, BioISI \u2013 Biosystems & Integrative Sciences Institute, Plant Biology Department, Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, 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