{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:01:35Z","timestamp":1792814495447,"version":"4.5.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,1,1]]},"DOI":"10.1095\/biolreprod.104.032490","type":"journal-article","created":{"date-parts":[[2004,10,20]],"date-time":"2004-10-20T18:42:22Z","timestamp":1098297742000},"page":"22-32","source":"Crossref","is-referenced-by-count":97,"title":["Tyrosine Phosphorylation of the A Kinase Anchoring Protein 3 (AKAP3) and Soluble Adenylate Cyclase Are Involved in the Increase of Human Sperm Motility by Bicarbonate1"],"prefix":"10.1093","volume":"72","author":[{"given":"M.","family":"Luconi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Porazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Ferruzzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Marchiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Forti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Baldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"key\n\t\t\t\t20170224033259_R1","first-page":"9699","article-title":"Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through direct activation of adenylate cyclase","volume":"260","year":"1986","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R2","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1095\/biolreprod44.5.806","article-title":"Bicarbonate:carbon-dioxide regulation of sperm capacitation, hyperactivated motility, and acrosome reactions","volume":"44","year":"1991","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R3","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0005-2736(92)90188-R","article-title":"The role of anion channel in the mechanism of acrosome reaction in bull spermatozoa","volume":"1109","year":"1992","journal-title":"Biochim Biophys Acta"},{"key":"key\n\t\t\t\t20170224033259_R4","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1093\/oxfordjournals.humrep.a137807","article-title":"Regulation of cyclic adenosine monophosphate synthesis in human ejaculated spermatozoa. II. The role of calcium and bicarbonate ions on the activation of adenylyl cyclase","volume":"7","year":"1992","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20170224033259_R5","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1095\/biolreprod52.3.540","article-title":"Bicarbonate\/CO2 is not required for zona pellucida- or progesterone induced acrosomal exocytosis of mouse spermatozoa but is essential for capacitation","volume":"52","year":"1995","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R6","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/j.1939-4640.1995.tb00523.x","article-title":"Importance of bicarbonate to the progesterone-initiated human sperm acrosome reaction","volume":"16","year":"1995","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224033259_R7","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1242\/dev.121.4.1129","article-title":"Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation","volume":"121","year":"1995","journal-title":"Development"},{"key":"key\n\t\t\t\t20170224033259_R8","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1242\/dev.121.4.1139","article-title":"Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway","volume":"121","year":"1995","journal-title":"Development"},{"key":"key\n\t\t\t\t20170224033259_R9","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1095\/biolreprod61.1.76","article-title":"Roles of bicarbonate, cAMP, and protein tyrosine phosphorylation on capacitation and the spontaneous acrosome reaction of hamster sperm","volume":"61","year":"1999","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R10","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1093\/molehr\/5.11.1017","article-title":"Regulation of human sperm capacitation by a cholesterol efflux-stimulated signal transduction pathway leading to protein kinase A-mediated up-regulation of protein tyrosine phosphorylation","volume":"5","year":"1999","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20170224033259_R11","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1242\/dev.127.11.2407","article-title":"The capacitating agent bicarbonate induces protein kinase A-dependent changes in phospholipid transbilayer behavior in the sperm plasma membrane","volume":"127","year":"2000","journal-title":"Development"},{"key":"key\n\t\t\t\t20170224033259_R12","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0165-0378(01)00103-6","article-title":"Novel signaling pathways involved in sperm acquisition of fertilizing capacity","volume":"53","year":"2002","journal-title":"J Reprod Immunol"},{"key":"key\n\t\t\t\t20170224033259_R13","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1242\/dev.00353","article-title":"Bicarbonate actions on flagellar and Ca2+-channel responses: initial events in sperm activation","volume":"130","year":"2003","journal-title":"Development"},{"key":"key\n\t\t\t\t20170224033259_R14","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1095\/biolreprod.103.022822","article-title":"Bicarbonate is required for migration of sperm epididymal protein DE (CRISP-1) to the equatorial segment and expression of rat sperm fusion ability","volume":"70","year":"2004","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R15","doi-asserted-by":"crossref","first-page":"15847","DOI":"10.1073\/pnas.2536127100","article-title":"Anion exchanger 2 is essential for spermiogenesis in mice","volume":"100","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224033259_R16","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1046\/j.1439-0531.2003.00400.x","article-title":"Cyclic AMP signalling during mammalian sperm capacitation\u2014still largely terra incognita","volume":"38","year":"2003","journal-title":"Reprod Domest Anim"},{"key":"key\n\t\t\t\t20170224033259_R17","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1095\/biolreprod.102.012500","article-title":"Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells","volume":"68","year":"2003","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R18","doi-asserted-by":"crossref","first-page":"10676","DOI":"10.1073\/pnas.1831008100","article-title":"Calcium regulation of the soluble adenylyl cyclase expressed in mammalian spermatozoa","volume":"100","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224033259_R19","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/S1043-2760(01)00493-3","article-title":"A-kinase anchor proteins in endocrine system and reproduction","volume":"12","year":"2001","journal-title":"Trends Endocrinol Metab"},{"key":"key\n\t\t\t\t20170224033259_R20","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1242\/jcs.00931","article-title":"Increased phosphorylation of AKAP by inhibition of phosphatidylinositol 3-kinase enhances human sperm motility through tail recruitment of protein kinase A","volume":"117","year":"2004","journal-title":"J Cell Sci"},{"key":"key\n\t\t\t\t20170224033259_R21","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1095\/biolreprod52.6.1227","article-title":"Mouse sperm adenylyl cyclase: general properties and regulation by the zona pellucida","volume":"52","year":"1995","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R22","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/mrd.10344","article-title":"Evidence for multiple distinctly localized adenylyl cyclase isoforms in mammalian spermatozoa","volume":"66","year":"2003","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224033259_R23","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1126\/science.289.5479.625","article-title":"Soluble adenylate cyclase as an evolutionarily conserved bicarbonate sensor","volume":"289","year":"2000","journal-title":"Science"},{"key":"key\n\t\t\t\t20170224033259_R24","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/(SICI)1098-2795(200005)56:1<6::AID-MRD2>3.0.CO;2-M","article-title":"Specific expression of soluble adenylyl cyclase in male germ cells","volume":"56","year":"2000","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224033259_R25","doi-asserted-by":"crossref","first-page":"15922","DOI":"10.1074\/jbc.M212475200","article-title":"Kinetic properties of \u201csoluble\u201d adenylyl cyclase. Synergism between calcium and bicarbonate","volume":"278","year":"2003","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R26","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1096\/fj.02-0598fje","article-title":"Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains","volume":"17","year":"2003","journal-title":"FASEB J"},{"key":"key\n\t\t\t\t20170224033259_R27","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1083\/jcb.200311119","article-title":"Bicarbonate-responsive \u201csoluble\u201d adenylyl cyclase defines a nuclear cAMP microdomain","volume":"164","year":"2004","journal-title":"J Cell Biol"},{"key":"key\n\t\t\t\t20170224033259_R28","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1002\/j.1939-4640.2002.tb02314.x","article-title":"Activation of protein kinase A during human sperm capacitation and acrosome reaction","volume":"23","year":"2002","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224033259_R29","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1093\/molehr\/gag097","article-title":"First messenger regulation of capacitation via G protein coupled mechanisms: a tale of serendipity and discovery","volume":"9","year":"2003","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20170224033259_R30","first-page":"49523","article-title":"Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling","volume":"49","year":"2003","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R31","volume-title":"WHO laboratory manual for the examination of human semen and sperm-cervical mucus interactions","author":"World Health Organization (WHO)","year":"1999"},{"key":"key\n\t\t\t\t20170224033259_R32","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1093\/humrep\/16.9.1931","article-title":"Phosphatidylinositol 3-kinase inhibition enhances human sperm motility","volume":"16","year":"2001","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20170224033259_R33","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/j.1939-4640.2002.tb02279.x","article-title":"Bicarbonate stimulation of boar sperm motility via a protein kinase A-dependent pathway: between-cell and between-ejaculate differences are not due to deficiencies in protein kinase A activation","volume":"23","year":"2002","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224033259_R34","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1111\/j.1365-2605.2004.00440.x","article-title":"Phosphatidylinositol 3-kinase inhibition enhances human sperm motility and sperm-zona pellucida binding","volume":"27","year":"2004","journal-title":"Int J Androl"},{"key":"key\n\t\t\t\t20170224033259_R35","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1095\/biolreprod61.5.1184","article-title":"FSP95, a testis-specific 95-kilodalton fibrous sheath antigen that undergoes tyrosine phosphorylation in capacitated human spermatozoa","volume":"61","year":"1999","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R36","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1210\/mend.13.5.0278","article-title":"Isolation and molecular characterization of AKAP110, a novel, sperm-specific protein kinase A-anchoring protein","volume":"13","year":"1999","journal-title":"Mol Endocrinol"},{"key":"key\n\t\t\t\t20170224033259_R37","doi-asserted-by":"crossref","first-page":"11579","DOI":"10.1074\/jbc.M202325200","article-title":"Phosphoproteome analysis of capacitated human spermatozoa","volume":"278","year":"2003","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R38","doi-asserted-by":"crossref","first-page":"C803","DOI":"10.1152\/ajpcell.1992.262.4.C803","article-title":"Chemical probes for anion transporters of mammalian cell membranes","volume":"262","year":"1992","journal-title":"Am J Physiol Cell Physiol"},{"key":"key\n\t\t\t\t20170224033259_R39","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1242\/jcs.111.5.645","article-title":"A novel signal transduction cascade in capacitating human spermatozoa characterised by a redox-regulated, cAMP-mediated induction of tyrosine phosphorylation","volume":"111","year":"1998","journal-title":"J Cell Sci"},{"key":"key\n\t\t\t\t20170224033259_R40","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1095\/biolreprod56.3.707","article-title":"Regulation of protein tyrosine phosphorylation during bovine sperm capacitation by a cyclic adenosine 3\u20325\u2032-monophosphate-dependent pathway","volume":"56","year":"1997","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R41","doi-asserted-by":"crossref","first-page":"5241","DOI":"10.1016\/S0021-9258(17)37680-9","article-title":"A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002)","volume":"269","year":"1994","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R42","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1113\/jphysiol.1971.sp009400","article-title":"Micropuncture studies of the electrochemical aspects of fluid and electrolyte transport in individual seminiferous tubules, the epididymis and the vas deferens in rats","volume":"213","year":"1971","journal-title":"J Physiol"},{"key":"key\n\t\t\t\t20170224033259_R43","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/S0015-0282(16)42801-3","article-title":"Hydrogen ion and carbon dioxide content of the oviductal fluid of the rhesus monkey (Macaca mulatta)","volume":"28","year":"1977","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20170224033259_R44","first-page":"9699","article-title":"Lowered levels of bicarbonate in seminal plasma cause the poor sperm motility in human infertile patients","volume":"260","year":"1985","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R45","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1038\/ncb1047","article-title":"Involvement of CFTR in uterine bicarbonate secretion and the fertilizing capacity of sperm","volume":"5","year":"2003","journal-title":"Nat Cell Biol"},{"key":"key\n\t\t\t\t20170224033259_R46","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/j.1939-4640.2004.tb02787.x","article-title":"Immunolocalization and regulation of cystic fibrosis transmembrane conductance regulator in the adult rat epididymis","volume":"25","year":"2004","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224033259_R47","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0015-0282(16)39731-X","article-title":"Chemical composition of human oviduct fluid","volume":"24","year":"1973","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20170224033259_R48","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1530\/rep.0.1220519","article-title":"Hyperactivation of mammalian spermatozoa: function and regulation","volume":"122","year":"2001","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224033259_R49","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1002\/j.1939-4640.2000.tb02116.x","article-title":"CASA-practical aspects","volume":"21","year":"2000","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224033259_R50","doi-asserted-by":"crossref","first-page":"7001","DOI":"10.1074\/jbc.M206284200","article-title":"Involvement of a Na+\/HCO-3 cotransporter in mouse sperm capacitation","volume":"278","year":"2003","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R51","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0014-2999(99)00012-6","article-title":"Rapid inhibition of rat brain mitochondrial proton F0F1-ATPase activity by estrogens: comparison with Na+, K+-ATPase of porcine cortex","volume":"368","year":"1999","journal-title":"Eur J Pharm"},{"key":"key\n\t\t\t\t20170224033259_R52","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1073\/pnas.0400050101","article-title":"Mice deficient for soluble adenylyl cyclase are infertile because of a severe sperm-motility defect","volume":"101","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224033259_R53","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1095\/biolreprod61.1.240","article-title":"Role of tyrosine phosphorylation of flagellar proteins in hamster sperm hyperactivation","volume":"61","year":"1999","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R54","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1006\/dbio.2002.0728","article-title":"Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility","volume":"248","year":"2002","journal-title":"Dev Biol"},{"key":"key\n\t\t\t\t20170224033259_R55","doi-asserted-by":"crossref","first-page":"4747","DOI":"10.1074\/jbc.272.8.4747","article-title":"Protein kinase A-anchoring inhibitor peptides arrest mammalian sperm motility","volume":"272","year":"1997","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224033259_R56","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1095\/biolreprod.102.011106","article-title":"Phosphorylation of protein tyrosine residues in fresh and cryopreserved stallion spermatozoa under capacitating conditions","volume":"68","year":"2003","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R57","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1095\/biolreprod55.3.684","article-title":"Cyclic adenosine 3\u2032,5\u2032monophosphate-dependent regulation of protein tyrosine phosphorylation in relation to human sperm capacitation and motility","volume":"55","year":"1996","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224033259_R58","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1530\/rep.0.1260183","article-title":"Involvement of tyrosine kinase and cAMP-dependent kinase cross-talk in the regulation of human sperm motility","volume":"126","year":"2003","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224033259_R59","first-page":"787","article-title":"Capacitation induces tyrosine phosphorylation of proteins in the boar sperm plasma membrane","volume":"262","year":"1999","journal-title":"BBRC"},{"key":"key\n\t\t\t\t20170224033259_R60","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1002\/(SICI)1098-2795(200002)55:2<220::AID-MRD12>3.0.CO;2-I","article-title":"cAMP-dependent protein kinase control of plasma membrane lipid architecture in boar sperm","volume":"55","year":"2000","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224033259_R61","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/mrd.20028","article-title":"Rapid PKA-catalysed phosphorylation of boar sperm proteins induced by the capacitating agent bicarbonate","volume":"67","year":"2004","journal-title":"Mol Reprod Dev"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/biolreprod\/article-pdf\/72\/1\/22\/10558926\/biolreprod0022.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T02:31:28Z","timestamp":1734575488000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-lookup\/doi\/10.1095\/biolreprod.104.032490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1,1]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1,1]]}},"URL":"https:\/\/doi.org\/10.1095\/biolreprod.104.032490","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1,1]]}}}