{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T22:58:21Z","timestamp":1777157901843,"version":"3.51.4"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012166","name":"973 program","doi-asserted-by":"crossref","award":["2006CB504002"],"award-info":[{"award-number":["2006CB504002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"973 program","doi-asserted-by":"crossref","award":["2006CB944002"],"award-info":[{"award-number":["2006CB944002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Chinese Academy of Sciences Knowledge Innovation Program","award":["KSCX1-YW-R-54"],"award-info":[{"award-number":["KSCX1-YW-R-54"]}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We describe a novel epididymis-specific cDNA named Glb1l4, which was isolated from rat epididymis by differential display of mRNAs. Glb1l4 cDNA contains 2607 nucleotides and encodes a 637-amino acid protein with 50% similarity to mouse beta-galactosidase. The gene is located on chromosome 8q13, spanning 21 exons. Northern blot analysis reveals that Glb1l4 is specifically expressed in the caput region of epididymis and upregulated by androgen. A specific polyclonal antiserum against the N-terminal peptide of GLB1L4 has been produced. Western blot analysis and immunohistochemistry assay reveal that GLB1L4 is specifically expressed in the principal cells of the caput epididymis. Interestingly, its expression peaks at Postnatal Day 45 in mRNA level and at Postnatal Day 60 in protein level while the epididymis column cells undergo differentiation. Moreover, within this very period this secretory protein is confined inside the cell with a change of subcellular distribution pattern, which implies its important roles in the cell differentiation process. Only after the epididymal epithelium differentiation is completed and the spermatozoa enter the epididymal lumen is the GLB1L4 secreted into the luminal fluid and bound on the sperm head. Our results suggest that GLB1L4 may play various roles in principal cell differentiation and sperm maturation.<\/jats:p>","DOI":"10.1095\/biolreprod.108.071589","type":"journal-article","created":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T16:04:44Z","timestamp":1229616284000},"page":"696-706","source":"Crossref","is-referenced-by-count":14,"title":["The Novel Epididymis-Specific Beta-Galactosidase-Like Gene <i>Glb1l4<\/i> Is Essential in Epididymal Development and Sperm Maturation in Rats"],"prefix":"10.1093","volume":"80","author":[{"given":"Wei","family":"Zhen","sequence":"first","affiliation":[{"name":"3Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"},{"name":"5 The Graduate School of the Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"3Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"He","sequence":"additional","affiliation":[{"name":"3Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juyuan","family":"Guo","sequence":"additional","affiliation":[{"name":"3Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Lian","family":"Zhang","sequence":"additional","affiliation":[{"name":"3Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"},{"name":"4 Shanghai Institute of Planned Parenthood Research, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,4,1]]},"reference":[{"key":"2024041208161407100_R01","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/978-1-4615-0679-9_14","article-title":"The development of the epididymis.","volume-title":"The Epididymis: From Molecules to Clinical Practice","author":"Rodriguez","year":"2002"},{"key":"2024041208161407100_R02","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/j.1939-4640.1995.tb00548.x","article-title":"Specialized gene expression in the epididymis","volume":"16","author":"Connwall","year":"1995","journal-title":"J Androl"},{"key":"2024041208161407100_R03","first-page":"259","article-title":"Structure and function of the epithelium lining the ductuli efferents, ducts epididymis and ductus deferens in the rat.","volume-title":"Handbook of Physiology, vol. 5","author":"Hamilton","year":"1975"},{"key":"2024041208161407100_R04","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/j.1939-4640.1987.tb00962.x","article-title":"Morphometric analysis of the epididymis from normal and vasectomized rats","volume":"8","author":"Miler","year":"1987","journal-title":"J Androl"},{"key":"2024041208161407100_R05","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1111\/j.1365-2605.1978.tb00512.x","article-title":"Electrolyte and water transport in rat epididymis: its possible role in sperm maturation","volume":"1","author":"Wong","year":"1978","journal-title":"Int J Androl"},{"key":"2024041208161407100_R06","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1530\/rep.0.1220041","article-title":"Regulation of gene transcription in the epididymis","volume":"122","author":"Rodriguez","year":"2001","journal-title":"Reproduction"},{"key":"2024041208161407100_R07","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1365-2605.1993.tb01153.x","article-title":"Tissue-specific gene expression as an indicator of epididymis-specific functional status in the boar, bull and stallion","volume":"16","author":"Uhlenbruck","year":"1993","journal-title":"Int J Androl"},{"key":"2024041208161407100_R08","first-page":"89","article-title":"Glycolipid and glycoprotein degradation","volume":"60","author":"Conzelmann","year":"1987","journal-title":"Adv Enzymol Relat Areas Mol Biol"},{"key":"2024041208161407100_R09","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1042\/bj0710318","article-title":"Mammalian glycosidases; 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