{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:17:09Z","timestamp":1791058629888,"version":"4.1.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"DOI":"10.1095\/biolreprod.108.074880","type":"journal-article","created":{"date-parts":[[2009,2,4]],"date-time":"2009-02-04T22:05:16Z","timestamp":1233785116000},"page":"199-206","source":"Crossref","is-referenced-by-count":124,"title":["Hyperactivation of Stallion Sperm Is Required for Successful In Vitro Fertilization of Equine Oocytes1"],"prefix":"10.1093","volume":"81","author":[{"given":"L.A.","family":"McPartlin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.S.","family":"Suarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.A.","family":"Czaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Hinrichs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.J.","family":"Bedford-Guaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"key\n\t\t\t\t20170224031253_R01","first-page":"375","article-title":"In vitro fertilization in the horse. A retrospective study","volume":"44","year":"1991","journal-title":"J Reprod Fertil Suppl"},{"key":"key\n\t\t\t\t20170224031253_R02","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/S0093-691X(01)00610-0","article-title":"Effect of sperm cryopreservation and treatment with calcium ionophore or heparin on in vitro fertilization of horse oocytes","volume":"56","year":"2001","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R03","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/0093-691X(94)90448-R","article-title":"In vitro fertilization rate of horse oocytes with partially removed zonae","volume":"42","year":"1994","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R04","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1016\/S0093-691X(97)00095-2","article-title":"Intracytoplasmic sperm injection (ICSI) versus conventional IVF on abattoir-derived and in vitro-matured equine oocytes","volume":"47","year":"1997","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R05","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1093\/humrep\/12.12.2766","article-title":"Effects of follicular fluid supplementation of in-vitro maturation medium on the fertilization and development of equine oocytes after in-vitro fertilization or intracytoplasmic sperm injection","volume":"12","year":"1997","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20170224031253_R06","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/0093-691X(95)00402-T","article-title":"In vitro maturation and fertilization of equine oocytes recovered during the breeding season","volume":"45","year":"1996","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R07","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/mrd.1080260411","article-title":"In vitro fertilization of horse follicular oocytes matured in vitro","volume":"26","year":"1990","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224031253_R08","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1095\/biolreprod67.1.256","article-title":"In vitro fertilization of in vitro-matured equine oocytes: effect of maturation medium, duration of maturation, and sperm calcium ionophore treatment, and comparison with rates of fertilization in vivo after oviductal transfer","volume":"67","year":"2002","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R09","first-page":"189","article-title":"Mammalian fertilization.","volume-title":"The Physiology of Reproduction","year":"1994"},{"key":"key\n\t\t\t\t20170224031253_R10","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0165-0378(01)00103-6","article-title":"Novel signaling pathways involved in sperm acquisition of fertilizing capacity","volume":"53","year":"2002","journal-title":"J Reprod Immunol"},{"key":"key\n\t\t\t\t20170224031253_R11","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.2460\/ajvr.1993.54.09.1505","article-title":"Capacitation-like membrane changes and prolonged viability in vitro of equine spermatozoa cultured with uterine tube epithelial cells","volume":"54","year":"1993","journal-title":"Am J Vet Res"},{"key":"key\n\t\t\t\t20170224031253_R12","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1095\/biolreprod.102.011106","article-title":"Phosphorylation of protein tyrosine residues in fresh and cryopreserved stallion spermatozoa under capacitating conditions","volume":"68","year":"2003","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R13","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1002\/mrd.10216","article-title":"Progesterone induces acrosome reaction in stallion spermatozoa via a protein tyrosine kinase dependent pathway","volume":"64","year":"2003","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224031253_R14","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.2460\/ajvr.1987.48.09.1383","article-title":"Induction and characterization of acrosome reaction in equine spermatozoa","volume":"48","year":"1987","journal-title":"Am J Vet Res"},{"key":"key\n\t\t\t\t20170224031253_R15","first-page":"739","article-title":"Induction of acrosome reactions in stallion sperm by equine zona pellucida, porcine zona pellucida, and progesterone","volume":"1","year":"1995","journal-title":"Biol Reprod Mono"},{"key":"key\n\t\t\t\t20170224031253_R16","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/0093-691X(96)00075-1","article-title":"In vitro induction of acrosome reactions in stallion spermatozoa by heparin and A23187","volume":"45","year":"1996","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R17","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.theriogenology.2007.11.016","article-title":"A defined medium supports changes consistent with capacitation in stallion sperm, as evidenced by increases in protein tyrosine phosphorylation and high rates of acrosomal exocytosis","volume":"69","year":"2008","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R18","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1073\/pnas.0610286104","article-title":"All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility","volume":"104","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224031253_R19","doi-asserted-by":"crossref","first-page":"14869","DOI":"10.1073\/pnas.2136654100","article-title":"Hyperactivated sperm motility driven by CatSper2 is required for fertilization","volume":"100","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224031253_R20","first-page":"351","article-title":"Hyperactivation of mammalian sperm","volume":"49","year":"2003","journal-title":"Cell Mol Biol (Noisy-le-grand"},{"key":"key\n\t\t\t\t20170224031253_R21","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1095\/biolreprod46.5.779","article-title":"Hyperactivated sperm progress in the mouse oviduct","volume":"46","year":"1992","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R22","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/j.1939-4640.1992.tb01631.x","article-title":"Movement characteristics of boar sperm obtained from the oviduct or hyperactivated in vitro","volume":"13","year":"1992","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224031253_R23","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1093\/humupd\/dmi047","article-title":"Sperm transport in the female reproductive tract","volume":"12","year":"2006","journal-title":"Hum Reprod Update"},{"key":"key\n\t\t\t\t20170224031253_R24","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1046\/j.1439-0531.2003.00397.x","article-title":"Hyperactivated motility in sperm","volume":"38","year":"2003","journal-title":"Reprod Domest Anim"},{"key":"key\n\t\t\t\t20170224031253_R25","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1095\/biolreprod36.5.1191","article-title":"Initiation of hyperactivated flagellar bending in mouse sperm within the female reproductive tract","volume":"36","year":"1987","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R26","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1095\/biolreprod56.2.447","article-title":"Distribution of mucus and sperm in bovine oviducts after artificial insemination: the physical environment of the oviductal sperm reservoir","volume":"56","year":"1997","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R27","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1093\/humupd\/dmn029","article-title":"Control of hyperactivation in sperm","volume":"14","year":"2008","journal-title":"Hum Reprod Update"},{"key":"key\n\t\t\t\t20170224031253_R28","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1002\/j.1939-4640.1990.tb03228.x","article-title":"Kinematics of human spermatozoa incubated under capacitating conditions","volume":"11","year":"1990","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224031253_R29","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1095\/biolreprod65.5.1606","article-title":"An inositol 1,4,5-trisphosphate receptor-gated intracellular Ca(2+) store is involved in regulating sperm hyperactivated motility","volume":"65","year":"2001","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R30","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1530\/rep.0.1220519","article-title":"Hyperactivation of mammalian spermatozoa: function and regulation","volume":"122","year":"2001","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224031253_R31","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1095\/biolreprod61.1.76","article-title":"Roles of bicarbonate, cAMP, and protein tyrosine phosphorylation on capacitation and the spontaneous acrosome reaction of hamster sperm","volume":"61","year":"1999","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R32","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1242\/dev.121.4.1139","article-title":"Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway","volume":"121","year":"1995","journal-title":"Development"},{"key":"key\n\t\t\t\t20170224031253_R33","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1095\/biolreprod.103.026476","article-title":"Different signaling pathways in bovine sperm regulate capacitation and hyperactivation","volume":"70","year":"2004","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R34","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/mrd.1080380309","article-title":"Mechanism for procaine-mediated hyperactivated motility in guinea pig spermatozoa","volume":"38","year":"1994","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224031253_R35","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1095\/biolreprod65.2.462","article-title":"Evaluation of in vitro capacitation of stallion spermatozoa","volume":"65","year":"2001","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1095\/biolreprod.103.025809","article-title":"Requirements for glucose beyond sperm capacitation during in vitro fertilization in the mouse","volume":"71","year":"2004","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R37","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/S0022-2275(20)38064-0","article-title":"Surface modification of Beckman Ultra-Clear centrifuge tubes for density gradient centrifugation of lipoproteins","volume":"23","year":"1982","journal-title":"J Lipid Res"},{"key":"key\n\t\t\t\t20170224031253_R38","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","year":"1970","journal-title":"Nature"},{"key":"key\n\t\t\t\t20170224031253_R39","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0093-691X(02)01133-0","article-title":"Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes","volume":"59","year":"2003","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R40","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1095\/biolreprod.104.036012","article-title":"Chromatin configuration within the germinal vesicle of horse oocytes: changes post mortem and relationship to meiotic and developmental competence","volume":"72","year":"2005","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R41","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1242\/dev.121.4.1129","article-title":"Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation","volume":"121","year":"1995","journal-title":"Development"},{"key":"key\n\t\t\t\t20170224031253_R42","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.devcel.2005.06.007","article-title":"The \u201csoluble\u201d adenylyl cyclase in sperm mediates multiple signaling events required for fertilization","volume":"9","year":"2005","journal-title":"Dev Cell"},{"key":"key\n\t\t\t\t20170224031253_R43","doi-asserted-by":"crossref","first-page":"13483","DOI":"10.1073\/pnas.0405580101","article-title":"Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility","volume":"101","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224031253_R44","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1093\/molehr\/5.11.1017","article-title":"Regulation of human sperm capacitation by a cholesterol efflux-stimulated signal transduction pathway leading to protein kinase A-mediated up-regulation of protein tyrosine phosphorylation","volume":"5","year":"1999","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20170224031253_R45","doi-asserted-by":"crossref","first-page":"459","DOI":"10.2164\/jandrol.05107","article-title":"Hyperactivated motility in rhesus macaque (Macaca mulatta) spermatozoa","volume":"27","year":"2006","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224031253_R46","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.ydbio.2006.11.007","article-title":"Contributions of extracellular and intracellular Ca2+ to regulation of sperm motility: release of intracellular stores can hyperactivate CatSper1 and CatSper2 null sperm","volume":"303","year":"2007","journal-title":"Dev Biol"},{"key":"key\n\t\t\t\t20170224031253_R47","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1095\/biolreprod.106.055038","article-title":"Bovine sperm hyperactivation is promoted by alkaline-stimulated Ca2+ influx","volume":"76","year":"2007","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R48","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1439-0272.2006.00751.x","article-title":"The influence of homogenous zona pellucida on human spermatozoa hyperactivation, acrosome reaction and zona binding","volume":"39","year":"2007","journal-title":"Andrologia"},{"key":"key\n\t\t\t\t20170224031253_R49","doi-asserted-by":"crossref","first-page":"32238","DOI":"10.1074\/jbc.M501430200","article-title":"Identical phenotypes of CatSper1 and CatSper2 null mice","volume":"280","year":"2005","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224031253_R50","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1530\/rep.1.00770","article-title":"Signal transduction mechanisms involved in in vitro ram sperm capacitation","volume":"132","year":"2006","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224031253_R51","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1095\/biolreprod.106.054627","article-title":"Kinetics of the progesterone-induced acrosome reaction and its relation to intracellular calcium responses in individual human spermatozoa","volume":"75","year":"2006","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R52","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/j.1939-4640.1998.tb01994.x","article-title":"The molecular basis of sperm capacitation","volume":"19","year":"1998","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224031253_R53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod59.1.1","article-title":"Regulation of protein phosphorylation during sperm capacitation","volume":"59","year":"1998","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224031253_R54","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/S0093-691X(01)00620-3","article-title":"Penetration of zona-free hamster, bovine and equine oocytes by stallion and bull spermatozoa pretreated with equine follicular fluid, dilauroylphosphatidylcholine or calcium ionophore A23187","volume":"56","year":"2001","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R55","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1016\/S0093-691X(02)00659-3","article-title":"Capacitation and acrosomal exocytosis are enhanced by incubation of stallion spermatozoa in a commercial semen extender","volume":"57","year":"2002","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224031253_R56","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1073\/pnas.0306846101","article-title":"Long-term effects of culture of preimplantation mouse embryos on behavior","volume":"101","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224031253_R57","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.ydbio.2006.08.041","article-title":"Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term","volume":"300","year":"2006","journal-title":"Dev Biol"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/biolreprod\/article-pdf\/81\/1\/199\/10574780\/biolreprod0199.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T06:37:16Z","timestamp":1738910236000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-lookup\/doi\/10.1095\/biolreprod.108.074880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,1]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,7,1]]}},"URL":"https:\/\/doi.org\/10.1095\/biolreprod.108.074880","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,1]]}}}