{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:02:15Z","timestamp":1790722935468,"version":"4.1.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"DOI":"10.1095\/biolreprod.109.083196","type":"journal-article","created":{"date-parts":[[2010,2,18]],"date-time":"2010-02-18T03:12:09Z","timestamp":1266462729000},"page":"1119-1128","source":"Crossref","is-referenced-by-count":43,"title":["Assessing the Role of Claudins in Maintaining the Integrity of Epididymal Tight Junctions Using Novel Human Epididymal Cell Lines1"],"prefix":"10.1093","volume":"82","author":[{"given":"\u00c9vemie","family":"Dub\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Dufresne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter T.K.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Hermo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Cyr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"key\n\t\t\t\t20170224023639_R01","first-page":"999","article-title":"Efferent ducts, epididymis, and vas deferens: structure, functions, and their regulation.","volume-title":"The Physiology of Reproduction","year":"1988"},{"key":"key\n\t\t\t\t20170224023639_R02","first-page":"1071","article-title":"The epididymis.","volume-title":"Knobil and Neill's Physiology of Reproduction, 3rd ed","year":"2006"},{"key":"key\n\t\t\t\t20170224023639_R03","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/jemt.1070300106","article-title":"Epididymal epithelium: its contribution to the formation of a luminal fluid microenvironment","volume":"30","year":"1995","journal-title":"Microsc Res Tech"},{"key":"key\n\t\t\t\t20170224023639_R04","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1745-7262.2007.00308.x","article-title":"Orchestration of occludins, claudins, catenins and cadherins as players involved in maintenance of the blood-epididymal barrier in animals and humans","volume":"9","year":"2007","journal-title":"Asian J Androl"},{"key":"key\n\t\t\t\t20170224023639_R05","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1083\/jcb.53.3.758","article-title":"Variations in tight and gap junctions in mammalian tissues","volume":"53","year":"1972","journal-title":"J Cell Biol"},{"key":"key\n\t\t\t\t20170224023639_R06","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0012-1606(78)90137-9","article-title":"Development of tight junctions in the caput epididymal epithelium of the mouse","volume":"63","year":"1978","journal-title":"Dev Biol"},{"key":"key\n\t\t\t\t20170224023639_R07","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1002\/ar.1091910409","article-title":"Regional differentiation of cell junctions in the excurrent duct epithelium of the rat testis as revealed by freeze-fracture","volume":"191","year":"1978","journal-title":"Anat Rec"},{"key":"key\n\t\t\t\t20170224023639_R08","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1530\/jrf.0.0630095","article-title":"Permeability characteristics of the epithelium in the rat caput epididymidis","volume":"63","year":"1981","journal-title":"J Reprod Fertil"},{"key":"key\n\t\t\t\t20170224023639_R09","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1095\/biolreprod.106.059246","article-title":"Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis","volume":"76","year":"2007","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224023639_R10","first-page":"854","article-title":"Claudin-1 is not restricted to tight junctions in the rat epididymis","volume":"142","year":"2001","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t20170224023639_R11","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/mrd.20467","article-title":"Identification of multiple claudins in the rat epididymis","volume":"73","year":"2006","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224023639_R12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1679\/aohc.68.213","article-title":"Segment-specific expression of tight junction proteins, claudin-2 and -10, in the rat epididymal epithelium","volume":"68","year":"2005","journal-title":"Arch Histol Cytol"},{"key":"key\n\t\t\t\t20170224023639_R13","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1002\/ar.20597","article-title":"Claudin-7 expressed on lateral membrane of rat epididymal epithelium does not form aberrant tight junction strands","volume":"290","year":"2007","journal-title":"Anat Rec (Hoboken"},{"key":"key\n\t\t\t\t20170224023639_R14","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1095\/biolreprod.105.039719","article-title":"The mouse epididymal transcriptome: transcriptional profiling of segmental gene expression in the epididymis","volume":"73","year":"2005","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224023639_R15","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1083\/jcb.143.2.391","article-title":"A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts","volume":"143","year":"1998","journal-title":"J Cell Biol"},{"key":"key\n\t\t\t\t20170224023639_R16","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1146\/annurev.physiol.68.040104.131404","article-title":"Claudins and epithelial paracellular transport","volume":"68","year":"2006","journal-title":"Annu Rev Physiol"},{"key":"key\n\t\t\t\t20170224023639_R17","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1242\/jcs.01235","article-title":"Barriers built on claudins","volume":"117","year":"2004","journal-title":"J Cell Sci"},{"key":"key\n\t\t\t\t20170224023639_R18","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S0015-0282(16)54469-0","article-title":"Fecundity and infertility in the United States: incidence and trends","volume":"56","year":"1991","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20170224023639_R19","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0028-2243(91)90206-Z","article-title":"Prevalence of infertility: international data and problems of measurement","volume":"38","year":"1991","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"key\n\t\t\t\t20170224023639_R20","first-page":"609","article-title":"Male infertility.","volume-title":"Campbell's Urology, 8th ed","year":"2006"},{"key":"key\n\t\t\t\t20170224023639_R21","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1095\/biolreprod.107.062760","article-title":"Alterations in gene expression in the caput epididymides of nonobstructive azoospermic men","volume":"78","year":"2008","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224023639_R22","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.bbamem.2007.11.007","article-title":"Interactions of tight junctions with membrane channels and transporters","volume":"1778","year":"2008","journal-title":"Biochim Biophys Acta"},{"key":"key\n\t\t\t\t20170224023639_R23","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.bbamem.2007.10.018","article-title":"Structure and function of claudins","volume":"1778","year":"2008","journal-title":"Biochim Biophys Acta"},{"key":"key\n\t\t\t\t20170224023639_R24","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1530\/jrf.0.0900081","article-title":"Maintenance of human epididymal epithelial cell function in monolayer culture","volume":"90","year":"1990","journal-title":"J Reprod Fertil"},{"key":"key\n\t\t\t\t20170224023639_R25","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1093\/humrep\/12.3.514","article-title":"Prolonged survival of human spermatozoa when co-incubated with epididymal cell cultures","volume":"12","year":"1997","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20170224023639_R26","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/S0015-0282(16)55327-8","article-title":"The culture of human epididymal epithelium and in vitro maturation of epididymal spermatozoa","volume":"58","year":"1992","journal-title":"Fertil Steril"},{"issue":"(pt. 4)","key":"key\n\t\t\t\t20170224023639_R27","first-page":"687","article-title":"Ductal epithelial cells cultured from human foetal epididymis and vas deferens: relevance to sterility in cystic fibrosis","volume":"92","year":"1989","journal-title":"J Cell Sci"},{"key":"key\n\t\t\t\t20170224023639_R28","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1093\/molehr\/8.8.695","article-title":"The dog as a model to study human epididymal function at a molecular level","volume":"8","year":"2002","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20170224023639_R29","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2164\/jandrol.107.004119","article-title":"De Graaf's thread: the human epididymis","volume":"29","year":"2008","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224023639_R30","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1002\/j.1939-4640.2002.tb02344.x","article-title":"Immortalized epididymal cell lines from transgenic mice overexpressing temperature-sensitive simian virus 40 large T-antigen gene","volume":"23","year":"2002","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224023639_R31","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.mce.2004.06.010","article-title":"Spontaneously immortalized epithelial cells from mouse caput epididymidis","volume":"224","year":"2004","journal-title":"Mol Cell Endocrinol"},{"key":"key\n\t\t\t\t20170224023639_R32","doi-asserted-by":"crossref","first-page":"4710","DOI":"10.1210\/en.2004-1634","article-title":"Characterization of a novel rat epididymal cell line to study epididymal function","volume":"146","year":"2005","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t20170224023639_R33","doi-asserted-by":"crossref","first-page":"36117","DOI":"10.1074\/jbc.M608853200","article-title":"Study of claudin function by RNA interference","volume":"281","year":"2006","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20170224023639_R34","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1530\/rep.1.00964","article-title":"Expression of ion transport-associated proteins in human efferent and epididymal ducts","volume":"133","year":"2007","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224023639_R35","doi-asserted-by":"crossref","first-page":"C198","DOI":"10.1152\/ajpcell.1995.269.1.C198","article-title":"Cloning, tissue distribution, and functional analysis of the human Na+\/N+ exchanger isoform, NHE3","volume":"269","year":"1995","journal-title":"Am J Physiol"},{"key":"key\n\t\t\t\t20170224023639_R36","first-page":"F1","article-title":"Molecular structure and assembly of the tight junction","volume":"274","year":"1998","journal-title":"Am J Physiol"},{"key":"key\n\t\t\t\t20170224023639_R37","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1071\/RD9960553","article-title":"Regulation of the motility and metabolism of spermatozoa for storage in the epididymis of eutherian and marsupial mammals","volume":"8","year":"1996","journal-title":"Reprod Fertil Dev"},{"key":"key\n\t\t\t\t20170224023639_R38","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1007\/BF00221479","article-title":"Immunohistochemical investigation of different cytokeratins and vimentin in the human epididymis from the fetal period up to adulthood","volume":"257","year":"1989","journal-title":"Cell Tissue Res"},{"key":"key\n\t\t\t\t20170224023639_R39","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1111\/j.1432-1033.1997.0440a.x","article-title":"Differential androgen regulation of the murine genes for cysteine-rich secretory proteins (CRISP)","volume":"250","year":"1997","journal-title":"Eur J Biochem"},{"key":"key\n\t\t\t\t20170224023639_R40","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/mrd.10115","article-title":"On the regulation of Crisp-1 mRNA expression and protein secretion by luminal factors presented in vivo by microperfusion of the rat proximal caput epididymidis","volume":"61","year":"2002","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224023639_R41","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0378-1119(03)00608-5","article-title":"Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2","volume":"312","year":"2003","journal-title":"Gene"},{"key":"key\n\t\t\t\t20170224023639_R42","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/j.1745-7262.2007.00316.x","article-title":"Androgenic regulation of novel genes in the epididymis","volume":"9","year":"2007","journal-title":"Asian J Androl"},{"key":"key\n\t\t\t\t20170224023639_R43","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1016\/S0015-0282(98)00094-6","article-title":"Regional distribution of 5alpha-reductase type 1 and type 2 mRNA along the human epididymis","volume":"69","year":"1998","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20170224023639_R44","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1093\/molehr\/3.11.933","article-title":"Region-specific expression of the androgen receptor in the human epididymis","volume":"3","year":"1997","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20170224023639_R45","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/molehr\/7.3.227","article-title":"Differential expression of oestrogen receptor alpha and beta proteins in the testes and male reproductive system of human and non-human primates","volume":"7","year":"2001","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20170224023639_R46","first-page":"317","article-title":"The role of estrogens in the endocrine and paracrine regulation of efferent ductules, epididymis and vas deferens.","volume-title":"The Epididymis: From Molecules to Clinical Practice","year":"2002"},{"key":"key\n\t\t\t\t20170224023639_R47","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1095\/biolreprod.106.057430","article-title":"Activation of an SP binding site is crucial for the expression of claudin 1 in rat epididymal principal cells","volume":"76","year":"2007","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224023639_R48","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.1073\/pnas.72.11.4487","article-title":"Structure of tight junctions in epithelia with different permeability","volume":"72","year":"1975","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224023639_R49","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1113\/jphysiol.1992.sp019311","article-title":"Electrophysiological studies of anion secretion in cultured human epididymal cells","volume":"455","year":"1992","journal-title":"J Physiol"},{"key":"key\n\t\t\t\t20170224023639_R50","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1095\/biolreprod52.1.192","article-title":"Regional differences in bioelectrical properties and anion secretion in cultured epithelia from rat and human male excurrent ducts","volume":"52","year":"1995","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224023639_R51","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1530\/jrf.0.0950385","article-title":"Polarized functions and permeability properties of rat epididymal epithelial cells in vitro","volume":"95","year":"1992","journal-title":"J Reprod Fertil"},{"key":"key\n\t\t\t\t20170224023639_R52","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1095\/biolreprod.102.009381","article-title":"Adenosine stimulates anion secretion across cultured and native adult human vas deferens epithelia","volume":"68","year":"2003","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224023639_R53","first-page":"a002584","article-title":"Physiology and function of the tight junction","volume":"1","year":"2009","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"key\n\t\t\t\t20170224023639_R54","doi-asserted-by":"crossref","first-page":"784","DOI":"10.2164\/jandrol.107.002980","article-title":"Structural alterations of epididymal epithelial cells in cathepsin A-deficient mice affect the blood-epididymal barrier and lead to altered sperm motility","volume":"28","year":"2007","journal-title":"J Androl"},{"key":"key\n\t\t\t\t20170224023639_R55","doi-asserted-by":"crossref","first-page":"2683","DOI":"10.1242\/jcs.02406","article-title":"Overexpression of claudin-7 decreases the paracellular Cl- conductance and increases the paracellular Na+ conductance in LLC-PK1 cells","volume":"118","year":"2005","journal-title":"J Cell Sci"},{"key":"key\n\t\t\t\t20170224023639_R56","first-page":"407","article-title":"Ultrastructure of the perfused rat epididymis: effect of luminal sodium ion concentration","volume":"226","year":"1982","journal-title":"Cell Tissue Res"},{"key":"key\n\t\t\t\t20170224023639_R57","first-page":"31","article-title":"The epididymis as a chloride-secreting organ","volume":"9","year":"1994","journal-title":"News Physiol Sci"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/biolreprod\/article-pdf\/82\/6\/1119\/10575217\/biolreprod1119.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,24]],"date-time":"2024-03-24T03:36:50Z","timestamp":1711251410000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-lookup\/doi\/10.1095\/biolreprod.109.083196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,1]]},"references-count":57,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6,1]]}},"URL":"https:\/\/doi.org\/10.1095\/biolreprod.109.083196","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,1]]}}}