{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:35:24Z","timestamp":1787009724438,"version":"build-2736575974"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"DOI":"10.1095\/biolreprod.113.115972","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T00:19:53Z","timestamp":1391041193000},"source":"Crossref","is-referenced-by-count":101,"title":["Differential Endometrial Cell Sensitivity to a Cholesterol-Dependent Cytolysin Links Trueperella pyogenes to Uterine Disease in Cattle1"],"prefix":"10.1093","volume":"90","author":[{"given":"Matthew R.","family":"Amos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth D.","family":"Healey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Goldstone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman M.","family":"Mahan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"D\u00fcvel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Joachim","family":"Schuberth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Sandra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Zieger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Dieuzy-Labaye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.E.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain Martin","family":"Sheldon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"key\n\t\t\t\t20170224040316_R01","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1095\/biolreprod.109.077370","article-title":"Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle","volume":"81","year":"2009","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224040316_R02","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1530\/rep.0.1230837","article-title":"Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle","volume":"123","year":"2002","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224040316_R03","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1530\/jrf.0.0290133","article-title":"A sequential histological study of the post-partum bovine uterus","volume":"29","year":"1972","journal-title":"J Reprod Fertil"},{"key":"key\n\t\t\t\t20170224040316_R04","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1530\/jrf.0.0180493","article-title":"Reproductive physiology of the post partum cow. l. Clinical histological findings","volume":"18","year":"1969","journal-title":"J Reprod Fertil"},{"key":"key\n\t\t\t\t20170224040316_R05","first-page":"168","article-title":"Endometrial biopsy in Holstein-Friesian dairy cows. III. Bacteriological analysis and correlations with histological findings","volume":"55","year":"1991","journal-title":"Can J Vet Res"},{"key":"key\n\t\t\t\t20170224040316_R06","doi-asserted-by":"crossref","first-page":"e9192","DOI":"10.1371\/journal.pone.0009192","article-title":"Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice","volume":"5","year":"2010","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20170224040316_R07","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1477-7827-7-55","article-title":"Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility","volume":"7","year":"2009","journal-title":"Reprod Biol Endocrinol"},{"key":"key\n\t\t\t\t20170224040316_R08","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1016\/j.theriogenology.2010.05.028","article-title":"A clinical approach to determine false positive findings of clinical endometritis by vaginoscopy by the use of uterine bacteriology and cytology in dairy cows","volume":"74","year":"2010","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224040316_R09","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s10482-005-2316-5","article-title":"Arcanobacterium pyogenes: molecular pathogenesis of an animal opportunist","volume":"88","year":"2005","journal-title":"Antonie Van Leeuwenhoek"},{"key":"key\n\t\t\t\t20170224040316_R10","doi-asserted-by":"crossref","first-page":"11315","DOI":"10.1073\/pnas.2033520100","article-title":"Redefining cholesterol's role in the mechanism of the cholesterol-dependent cytolysins","volume":"100","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224040316_R11","first-page":"38","article-title":"Cholesterol microcrystals and cochleate cylinders: attachment of pyolysin oligomers and domain 4","volume":"173","year":"2010","journal-title":"J Struct Biol"},{"key":"key\n\t\t\t\t20170224040316_R12","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1128\/IAI.67.4.1723-1728.1999","article-title":"An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence","volume":"67","year":"1999","journal-title":"Infect Immun"},{"key":"key\n\t\t\t\t20170224040316_R13","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1126\/science.1214935","article-title":"Disease tolerance as a defense strategy","volume":"335","year":"2012","journal-title":"Science"},{"key":"key\n\t\t\t\t20170224040316_R14","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.cell.2010.01.022","article-title":"Pattern recognition receptors and inflammation","volume":"140","year":"2010","journal-title":"Cell"},{"key":"key\n\t\t\t\t20170224040316_R15","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1073\/pnas.0435928100","article-title":"Recognition of pneumolysin by Toll-like receptor 4 confers resistance to pneumococcal infection","volume":"100","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224040316_R16","doi-asserted-by":"crossref","first-page":"e1001191","DOI":"10.1371\/journal.ppat.1001191","article-title":"Pneumolysin activates the NLRP3 inflammasome and promotes proinflammatory cytokines independently of TLR4","volume":"6","year":"2010","journal-title":"PLoS Pathog"},{"key":"key\n\t\t\t\t20170224040316_R17","first-page":"51","article-title":"Toll-like receptor 4 and MYD88-dependent signaling mechanisms of the innate immune system are essential for the response to lipopolysaccharide by epithelial and stromal cells of the bovine endometrium","volume":"86","year":"2012","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224040316_R18","doi-asserted-by":"crossref","first-page":"6100","DOI":"10.1128\/jb.179.19.6100-6106.1997","article-title":"The Arcanobacterium (Actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiol-activated cytolysin family","volume":"179","year":"1997","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20170224040316_R19","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.vetmic.2008.04.033","article-title":"Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis","volume":"132","year":"2008","journal-title":"Vet Microbiol"},{"key":"key\n\t\t\t\t20170224040316_R20","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1128\/AEM.01740-10","article-title":"Targeted allele replacement mutagenesis of Corynebacterium pseudotuberculosis","volume":"77","year":"2011","journal-title":"Appl Environ Microbiol"},{"key":"key\n\t\t\t\t20170224040316_R21","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1210\/en.2008-1379","article-title":"Bacterial lipopolysaccharide induces an endocrine switch from prostaglandin F2a to prostaglandin E2 in bovine endometrium","volume":"150","year":"2009","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t20170224040316_R22","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1111\/j.1600-0897.2012.01106.x","article-title":"Explants of intact endometrium to model bovine innate immunity and inflammation ex vivo","volume":"67","year":"2012","journal-title":"Am J Reprod Immunol"},{"key":"key\n\t\t\t\t20170224040316_R23","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3168\/jds.S0022-0302(80)82901-8","article-title":"Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum","volume":"63","year":"1980","journal-title":"J Dairy Sci"},{"key":"key\n\t\t\t\t20170224040316_R24","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","year":"1983","journal-title":"J Immunol Methods"},{"key":"key\n\t\t\t\t20170224040316_R25","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1530\/REP-07-0229","article-title":"Ovarian follicular cells have innate immune capabilities that modulate their endocrine function","volume":"134","year":"2007","journal-title":"Reproduction"},{"key":"key\n\t\t\t\t20170224040316_R26","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1136\/vr.110.5.96","article-title":"Effects of exogenous oestrogen and experimentally induced endometritis on absorption of sodium benzylpenicillin from the cow's uterus","volume":"110","year":"1982","journal-title":"Vet Rec"},{"key":"key\n\t\t\t\t20170224040316_R27","doi-asserted-by":"crossref","first-page":"W465","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny.fr: robust phylogenetic analysis for the non-specialist","volume":"36","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"key\n\t\t\t\t20170224040316_R28","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1128\/IAI.46.2.394-400.1984","article-title":"Isolation and identification of two hemolytic forms of streptolysin-O","volume":"46","year":"1984","journal-title":"Infect Immun"},{"key":"key\n\t\t\t\t20170224040316_R29","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S0171-2985(98)80051-X","article-title":"There is no regulatory role for induced nitric oxide in the regulation of the in vitro proliferative response of bovine mononuclear cells to mitogens, alloantigens or superantigens","volume":"198","year":"1998","journal-title":"Immunobiology"},{"key":"key\n\t\t\t\t20170224040316_R30","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1128\/JB.00694-10","article-title":"Inerolysin, a cholesterol-dependent cytolysin produced by Lactobacillus iners","volume":"193","year":"2011","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20170224040316_R31","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/j.bbamem.2007.03.026","article-title":"Use of cyclodextrins to manipulate plasma membrane cholesterol content: evidence, misconceptions and control strategies","volume":"1768","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"key\n\t\t\t\t20170224040316_R32","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1210\/en.2005-1113","article-title":"Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus","volume":"147","year":"2006","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t20170224040316_R33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.vetimm.2008.07.001","article-title":"Differential effects of alpha1-acid glycoprotein on bovine neutrophil respiratory burst activity and IL-8 production","volume":"126","year":"2008","journal-title":"Vet Immunol Immunopathol"},{"key":"key\n\t\t\t\t20170224040316_R34","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1016\/j.molimm.2008.06.010","article-title":"The chemokine CXCL3 is responsible for the constitutive chemotactic activity of bovine milk for neutrophils","volume":"45","year":"2008","journal-title":"Mol Immunol"},{"key":"key\n\t\t\t\t20170224040316_R35","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1084\/jem.20041215","article-title":"and other Gram-positive cytolysins are Toll-like receptor 4 agonists","volume":"200","year":"2004","journal-title":"J Exp Med"},{"key":"key\n\t\t\t\t20170224040316_R36","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.theriogenology.2004.03.017","article-title":"Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle","volume":"63","year":"2005","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224040316_R37","doi-asserted-by":"crossref","first-page":"e53048","DOI":"10.1371\/journal.pone.0053048","article-title":"Diversity and succession of bacterial communities in the uterine fluid of postpartum metritic, endometritic and healthy dairy cows","volume":"7","year":"2012","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20170224040316_R38","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.vetmic.2012.04.033","article-title":"Investigation of postpartum dairy cows' uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene","volume":"159","year":"2012","journal-title":"Vet Microbiol"},{"key":"key\n\t\t\t\t20170224040316_R39","doi-asserted-by":"crossref","first-page":"3629","DOI":"10.1128\/IAI.57.11.3629-3636.1989","article-title":"Listeriolysin O is essential for virulence of Listeria monocytogenes: direct evidence obtained by gene complementation","volume":"57","year":"1989","journal-title":"Infect Immun"},{"key":"key\n\t\t\t\t20170224040316_R40","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0882-4010(92)90111-Z","article-title":"Effect of insertional inactivation of the genes encoding pneumolysin and autolysin on the virulence of Streptococcus pneumoniae type 3","volume":"12","year":"1992","journal-title":"Microb Pathog"},{"key":"key\n\t\t\t\t20170224040316_R41","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.theriogenology.2007.04.056","article-title":"The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow","volume":"68","year":"2007","journal-title":"Theriogenology"},{"key":"key\n\t\t\t\t20170224040316_R42","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1182\/blood-2008-07-019307","article-title":"TLRs and innate immunity","volume":"113","year":"2009","journal-title":"Blood"},{"key":"key\n\t\t\t\t20170224040316_R43","article-title":"Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1 and TLR6","journal-title":"Endocrinology 2014"},{"key":"key\n\t\t\t\t20170224040316_R44","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/nri3167","article-title":"Beyond pattern recognition: five immune checkpoints for scaling the microbial threat","volume":"12","year":"2012","journal-title":"Nat Rev Immunol"},{"key":"key\n\t\t\t\t20170224040316_R45","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1038\/nri2873","article-title":"Sterile inflammation: sensing and reacting to damage","volume":"10","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"key\n\t\t\t\t20170224040316_R46","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1126\/science.1071059","article-title":"The danger model: a renewed sense of self","volume":"296","year":"2002","journal-title":"Science"},{"key":"key\n\t\t\t\t20170224040316_R47","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1111\/j.1462-5822.2006.00733.x","article-title":"Pore-forming Staphylococcus aureus alpha-toxin triggers epidermal growth factor receptor-dependent proliferation","volume":"8","year":"2006","journal-title":"Cell Microbiol"},{"key":"key\n\t\t\t\t20170224040316_R48","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.vetmic.2008.04.025","article-title":"Transcriptional regulation of pyolysin production in the animal pathogen, Arcanobacterium pyogenes","volume":"132","year":"2008","journal-title":"Vet Micro"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/biolreprod\/article-pdf\/90\/3\/54","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T17:08:54Z","timestamp":1597424934000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-lookup\/doi\/10.1095\/biolreprod.113.115972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,1]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3,1]]}},"URL":"https:\/\/doi.org\/10.1095\/biolreprod.113.115972","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,1]]}}}