{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:32:06Z","timestamp":1762673526108},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,10,1]]},"DOI":"10.1095\/biolreprod67.4.1225","type":"journal-article","created":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T19:17:03Z","timestamp":1132255023000},"page":"1225-1231","source":"Crossref","is-referenced-by-count":39,"title":["Expression and Structure-Function Analysis of DE, a Sperm Cysteine-Rich Secretory Protein That Mediates Gamete Fusion1"],"prefix":"10.1093","volume":"67","author":[{"given":"Diego A.","family":"Ellerman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanina G.","family":"Da Ros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De\u0301bora J.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolores","family":"Busso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro M.","family":"Morgenfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia S.","family":"Cuasnicu\u0301","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"key":"key\n\t\t\t\t20170224155505_R01","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1006\/dbio.1993.1166","article-title":"Molecular mechanisms of sperm-egg membrane binding and fusion in mammals.","volume":"158","year":"1993","journal-title":"Dev Biol"},{"key":"key\n\t\t\t\t20170224155505_R02","first-page":"D114","article-title":"Sperm disintegrins, egg integrins, and other cell adhesion molecules of mammalian gamete plasma membrane interactions.","volume":"4","year":"1999","journal-title":"Front Biosci"},{"key":"key\n\t\t\t\t20170224155505_R03","first-page":"317","article-title":"Androgen-controlled specific proteins in rat epididymis.","volume":"69","year":"1976","journal-title":"J Endocr"},{"key":"key\n\t\t\t\t20170224155505_R04","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0303-7207(79)90077-7","article-title":"Isolation and characterization of specific rat epididymal proteins.","volume":"13","year":"1979","journal-title":"Mol Cell Endocrinol"},{"key":"key\n\t\t\t\t20170224155505_R05","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1111\/j.1365-2605.1982.tb00296.x","article-title":"Carbohydrate composition of specific rat epididymal protein.","volume":"5","year":"1982","journal-title":"Int J Androl"},{"key":"key\n\t\t\t\t20170224155505_R06","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1095\/biolreprod23.1.181","article-title":"Distribution and site of production of specific proteins in the rat epididymis.","volume":"23","year":"1980","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224155505_R07","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1095\/biolreprod23.4.737","article-title":"Interaction of proteins of epididymal origin with spermatozoa.","volume":"23","year":"1980","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224155505_R08","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1002\/mrd.1080310107","article-title":"Redistribution of a rat sperm epididymal glycoprotein after in vivo and in vitro capacitation.","volume":"31","year":"1992","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224155505_R09","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1002\/(SICI)1098-2795(200006)56:2<180::AID-MRD9>3.0.CO;2-4","article-title":"Relationship between the association of rat epididymal protein DE with spermatozoa and the behavior and function of the protein.","volume":"56","year":"2000","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224155505_R10","first-page":"143","article-title":"Potential contraceptive use of an epididymal protein that participates in fertilization.","year":"1990","journal-title":"Gamete Interaction. Prospects for Immunocontraception"},{"key":"key\n\t\t\t\t20170224155505_R11","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0012-1606(92)90093-V","article-title":"Mammalian sperm-egg fusion: the rat egg has complementary sites for a sperm protein that mediates gamete fusion.","volume":"153","year":"1992","journal-title":"Dev Biol"},{"key":"key\n\t\t\t\t20170224155505_R12","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1095\/biolreprod55.1.200","article-title":"Mammalian sperm-egg fusion: the development of rat oolemma fusibility during oogenesis involves the appearance of binding sites for sperm protein \u201cDE\u201d.","volume":"55","year":"1996","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224155505_R13","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/257321a0","article-title":"Mosaicism in organisation of concanavalin A receptors on surface membranes of mouse eggs.","volume":"257","year":"1975","journal-title":"Nature"},{"key":"key\n\t\t\t\t20170224155505_R14","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/mrd.1120090404","article-title":"Changes at the hamster oocyte surface from germinal vesicle stage to ovulation.","volume":"9","year":"1984","journal-title":"Gamete Res"},{"key":"key\n\t\t\t\t20170224155505_R15","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0303-7207(92)90207-M","article-title":"Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule.","volume":"89","year":"1992","journal-title":"Mol Cell Endocrinol"},{"key":"key\n\t\t\t\t20170224155505_R16","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/mrd.1080420205","article-title":"Mouse androgen-dependent epididymal glycoprotein CRISP-1 (DE\/AEG): isolation, biochemical characterization, and expression in recombinant form.","volume":"42","year":"1995","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224155505_R17","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1006\/geno.1996.0131","article-title":"Characterization of a human glycoprotein with potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).","volume":"32","year":"1996","journal-title":"Genomics"},{"key":"key\n\t\t\t\t20170224155505_R18","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/j.1432-1033.1996.t01-1-00827.x","article-title":"The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3.","volume":"236","year":"1996","journal-title":"Eur J Biochem"},{"key":"key\n\t\t\t\t20170224155505_R19","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1095\/biolreprod63.2.462","article-title":"Mammalian sperm-egg fusion: evidence that epididymal protein DE plays a role in mouse gamete fusion.","volume":"63","year":"2000","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224155505_R20","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1095\/biolreprod65.4.1000","article-title":"Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg.","volume":"65","year":"2001","journal-title":"Biol Reprod"},{"key":"key\n\t\t\t\t20170224155505_R21","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/0888-7543(89)90019-0","article-title":"Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene.","volume":"5","year":"1989","journal-title":"Genomics"},{"key":"key\n\t\t\t\t20170224155505_R22","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/(SICI)1098-2795(199606)44:2<221::AID-MRD11>3.0.CO;2-5","article-title":"Autoantigen 1 of the guinea pig sperm acrosome is the homologue of mouse Tpx-1 and human TPX1 and is a member of the cysteine-rich secretory protein (CRISP) family.","volume":"44","year":"1996","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224155505_R23","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1006\/bbrc.1998.8918","article-title":"Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells.","volume":"248","year":"1998","journal-title":"Biochem Biophys Res Commun"},{"key":"key\n\t\t\t\t20170224155505_R24","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0014-5793(97)01514-7","article-title":"Biochemical and conformational characterisation of HSP-3, a stallion seminal plasma protein of the cysteine-rich secretory protein (CRISP) family.","volume":"420","year":"1997","journal-title":"FEBS Lett"},{"key":"key\n\t\t\t\t20170224155505_R25","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/S0167-4838(98)00122-8","article-title":"Equine CRISP-3: primary structure and expression in the male genital tract.","volume":"1387","year":"1998","journal-title":"Biochim Biophys Acta"},{"key":"key\n\t\t\t\t20170224155505_R26","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1210\/endo.133.1.8319566","article-title":"Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE\/AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland.","volume":"133","year":"1993","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t20170224155505_R27","doi-asserted-by":"crossref","first-page":"6160","DOI":"10.1128\/MCB.16.11.6160","article-title":"CRISP-3, a protein with homology to plant defense proteins, is expressed in mouse B cells under the control of Oct2.","volume":"16","year":"1996","journal-title":"Mol Cell Biol"},{"key":"key\n\t\t\t\t20170224155505_R28","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/0014-5793(96)00030-0","article-title":"SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein.","volume":"380","year":"1996","journal-title":"FEBS Lett"},{"key":"key\n\t\t\t\t20170224155505_R29","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0041-0101(90)90065-F","article-title":"Isolation and characterization of helothermine, a novel toxin from Heloderma horridum horridum (Mexican beaded lizard) venom.","volume":"28","year":"1990","journal-title":"Toxicon"},{"key":"key\n\t\t\t\t20170224155505_R30","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1016\/S0006-3495(95)80410-8","article-title":"Primary structure and properties of helothermine, a peptide that blocks ryanodine receptors.","volume":"68","year":"1995","journal-title":"Biophys J"},{"key":"key\n\t\t\t\t20170224155505_R31","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/S0041-0101(96)00192-4","article-title":"Cloning and expression of a cysteine-rich venom protein from Trimeresurus mucrosquamatus (Taiwan habu).","volume":"35","year":"1997","journal-title":"Toxicon"},{"key":"key\n\t\t\t\t20170224155505_R32","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1432-1033.1986.tb10118.x","article-title":"Molecular cloning of the cDNA for androgen-dependent sperm-coating glycoproteins secreted by the rat epididymis.","volume":"161","year":"1986","journal-title":"Eur J Biochem"},{"key":"key\n\t\t\t\t20170224155505_R33","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/mrd.1120010308","article-title":"Effect of elevated calcium concentration on fertilization of rat oocytes in vitro.","volume":"1","year":"1978","journal-title":"Gamete Res"},{"key":"key\n\t\t\t\t20170224155505_R34","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4.","volume":"227","year":"1970","journal-title":"Nature"},{"key":"key\n\t\t\t\t20170224155505_R35","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.","volume":"76","year":"1979","journal-title":"Proc Natl Acad Sci U S A"},{"key":"key\n\t\t\t\t20170224155505_R36","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1210\/endo-112-5-1590","article-title":"Androgen-controlled synthesis of specific protein in the rat epididymis.","volume":"112","year":"1983","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t20170224155505_R37","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1128\/mr.60.3.512-538.1996","article-title":"Strategies for achieving high-level expression of genes in Escherichia coli.","volume":"60","year":"1996","journal-title":"Microbiol Rev"},{"key":"key\n\t\t\t\t20170224155505_R38","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/S0167-7799(97)01155-4","article-title":"Strategies for optimizing heterologous protein expression in Escherichia coli.","volume":"16","year":"1998","journal-title":"Trends Biotechnol"},{"key":"key\n\t\t\t\t20170224155505_R39","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/S0958-1669(96)80012-7","article-title":"Expression of correctly folded proteins in Escherichia coli.","volume":"7","year":"1996","journal-title":"Curr Opin Biotechnol"},{"key":"key\n\t\t\t\t20170224155505_R40","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0958-1669(99)00003-8","article-title":"Recombinant protein expression in Escherichia coli.","volume":"10","year":"1999","journal-title":"Curr Opin Biotechnol"},{"key":"key\n\t\t\t\t20170224155505_R41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3480001","article-title":"Role of N-oligosaccharide endoplasmic reticulum processing reactions in glycoprotein folding and degradation.","volume":"348","year":"2000","journal-title":"Biochem J"},{"key":"key\n\t\t\t\t20170224155505_R42","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1083\/jcb.117.3.505","article-title":"Misfolding and aggregation of newly synthesized proteins in the endoplasmic reticulum.","volume":"117","year":"1992","journal-title":"J Cell Biol"},{"key":"key\n\t\t\t\t20170224155505_R43","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1530\/jrf.0.0670097","article-title":"Influence of incubation conditions, tunicamycin and castration on incorporation of [3H]mannose and [3H]fucose into rat epididymal glycoproteins in vitro.","volume":"67","year":"1983","journal-title":"J Reprod Fertil"},{"key":"key\n\t\t\t\t20170224155505_R44","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1002\/(SICI)1098-2795(199807)50:3<313::AID-MRD7>3.0.CO;2-M","article-title":"Identification of a rat testis-specific gene encoding a potential rat outer dense fibre protein.","volume":"50","year":"1998","journal-title":"Mol Reprod Dev"},{"key":"key\n\t\t\t\t20170224155505_R45","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1365-2958.1994.tb01281.x","article-title":"Building bridges: disulphide bond formation in the cell.","volume":"14","year":"1994","journal-title":"Mol Microbiol"},{"key":"key\n\t\t\t\t20170224155505_R46","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/mrd.1120070206","article-title":"Dithiothreitol, a disulfide-reducing agent, inhibits capacitation, acrosome reaction, and interaction with eggs by guinea pig spermatozoa.","volume":"7","year":"1983","journal-title":"Gamete Res"},{"key":"key\n\t\t\t\t20170224155505_R47","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/42484","article-title":"The putative chaperone calmegin is required for sperm fertility.","volume":"387","year":"1997","journal-title":"Nature"},{"key":"key\n\t\t\t\t20170224155505_R48","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0165-0378(95)00927-D","article-title":"Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition.","volume":"29","year":"1995","journal-title":"J Reprod Immunol"},{"key":"key\n\t\t\t\t20170224155505_R49","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1095\/biolreprod59.5.1029","article-title":"Potential contraceptive use of epididymal proteins: immunization of male rats with epididymal protein DE inhibits sperm fusion ability.","volume":"59","year":"1998","journal-title":"Biol Reprod"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/biolreprod\/article-pdf\/67\/4\/1225\/10558322\/biolreprod1225.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:48:20Z","timestamp":1626742100000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-lookup\/doi\/10.1095\/biolreprod67.4.1225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10,1]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,10,1]]}},"URL":"https:\/\/doi.org\/10.1095\/biolreprod67.4.1225","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10,1]]}}}