{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:49:12Z","timestamp":1762102152712,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[1998,10,1]],"date-time":"1998-10-01T00:00:00Z","timestamp":907200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:p>\n                    Melatonin has been suggested as a physiological antagonist of calmodulin. In this work, we have characterized melatonin binding sites in\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    oocyte membranes. Binding of [\n                    <jats:sup>125<\/jats:sup>\n                    I]melatonin by\n                    <jats:italic>X. laevis<\/jats:italic>\n                    oocyte membranes fulfills all criteria for binding to a receptor site. Binding was dependent on time, temperature, and membrane concentration and was stable, reversible, saturable, and specific. The binding site was also pharmacologically characterized. Stoichiometric studies showed a high\u2010affinity binding site with a K\n                    <jats:sub>d<\/jats:sub>\n                    of 1.18 nM. These data are in close agreement with data obtained from kinetic studies (K\n                    <jats:sub>d<\/jats:sub>\n                    =0.12 nM). In competition studies, we observed a low\u2010affinity binding site (K\n                    <jats:sub>d<\/jats:sub>\n                    =63.41 \u00b5M). Moreover, the binding site was characterized as calmodulin. Thus, binding was dependent on calcium and blocked by anti\u2010CaM antibodies in a concentration\u2010dependent manner. Calmodulin inhibitor chlorpromazine also inhibited binding of the tracer. From these results, it is suggested that membrane\u2010bound calmodulin acts as a melatonin binding site in\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    oocytes, where it might couple cellular activities to rhythmic circulating levels of melatonin. This hypothesis correlates with the previous findings describing melatonin as a physiological antagonist of calmodulin.\u2014Romero, M. P., Garc\u0131\u00b4a\u2010Pergan\u02dceda, A., Guerrero, J. M., Osuna, C. Membrane\u2010bound calmodulin in\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    oocytes as a novel binding site for melatonin.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    12, 1401\u20131408 (1998)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.12.13.1401","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T07:01:00Z","timestamp":1516258860000},"page":"1401-1408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Membrane\u2010bound calmodulin in\n                    <i>Xenopus laevis<\/i>\n                    oocytes as a novel binding site for melatonin"],"prefix":"10.1096","volume":"12","author":[{"given":"M. Paz","family":"Romero","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology The University of Seville School of Medicine Virgen Macarena Hospital  41009 Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Garc\u00eda\u2010Perga\u00f1eda","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology The University of Seville School of Medicine Virgen Macarena Hospital  41009 Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan M.","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology The University of Seville School of Medicine Virgen Macarena Hospital  41009 Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Osuna","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology The University of Seville School of Medicine Virgen Macarena Hospital  41009 Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.31.040191.003001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.13.7557023"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.11.6.9194527"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)61934-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"574","DOI":"10.4049\/jimmunol.159.2.574","article-title":"Melatonin enhances IL\u20102, IL\u20106, and IFN\u2010\u03b3 production by human circulating CD4+ cells","volume":"159","author":"Garc\u00eda\u2010Mauri\u00f1o S.","year":"1997","journal-title":"J. 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