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Entry of genetic material into mitochondria has been observed under both physiological and pathological conditions. We report here that double\u2010stranded DNA can move through a planar bilayer membrane containing isolated mitochondrial porin (voltage\u2010dependent anion channel). The transport is driven by the applied electrical field, and the presence of DNA is associated with a decrease of current conduction by the pores. The passage of DNA does not take place if the bilayer has not been doped with any protein or in the presence of both reconstituted porin and anti\u2010porin antibody. Translocation does not occur if the bilayer contains\n                    <jats:bold>\n                      <jats:italic>Shigella sonnei<\/jats:italic>\n                    <\/jats:bold>\n                    maltoporin, gramicidin A channels, or a 30 pS anion\u2010selective channel plus other proteins. These results show that mitochondrial porin is capable of mediating the transport of genetic material, revealing a new property of this molecule and futher confirming the idea that DNA can move through proteic pores.\u2014Szab\u00f2, I., B\u00e0thori, G., Tombola, F., Coppola, A., Schmehl, I., Brini, M., Ghazi, A., De Pinto, V., Zoratti, M. Double\u2010stranded DNA can be translocated across a planar membrane containing purified mitochondrial porin.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 12, 495\u2013502 (1998)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.12.6.495","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T07:46:31Z","timestamp":1516261591000},"page":"495-502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Double\u2010stranded DNA can be translocated across a planar membrane containing purified mitochondrial porin"],"prefix":"10.1096","volume":"12","author":[{"given":"Ildik\u00f2","family":"Szab\u00f2","sequence":"first","affiliation":[{"name":"CNR Unit for Biomembranes Department Biomedical Sciences University of Padova  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6rgy","family":"B\u00e0thori","sequence":"additional","affiliation":[{"name":"Institute of Biochemical and Pharmaceutical Sciences University of Catania  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Tombola","sequence":"additional","affiliation":[{"name":"Laboratory of Biomembranes ERS CNRS 571 University of Paris\u2010sud  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina","family":"Coppola","sequence":"additional","affiliation":[{"name":"Laboratory of Biomembranes ERS CNRS 571 University of Paris\u2010sud  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibolya","family":"Schmehl","sequence":"additional","affiliation":[{"name":"Laboratory of Biomembranes ERS CNRS 571 University of Paris\u2010sud  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Brini","sequence":"additional","affiliation":[{"name":"CNR Unit for Biomembranes Department Biomedical Sciences University of Padova  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Ghazi","sequence":"additional","affiliation":[{"name":"Department of Atomic Physics Eotvos University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito","family":"De Pinto","sequence":"additional","affiliation":[{"name":"Department of Atomic Physics Eotvos University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Zoratti","sequence":"additional","affiliation":[{"name":"Department of Atomic Physics Eotvos University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1998,4]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90455-8"},{"key":"e_1_2_2_3_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/BF00233944","article-title":"The ion channel behavior of the nuclear pore complex.","volume":"146","author":"Bustamante J. 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