{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:26:53Z","timestamp":1790857613436,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[1999,10,1]],"date-time":"1999-10-01T00:00:00Z","timestamp":938736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1999,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Human fibroblasts and keratinocytes possess nitric oxide synthases (NOS), which metabolize L\u2010arginine (L\u2010Arg) for producing nitric oxide (NO\n                    <jats:sup>\u2022<\/jats:sup>\n                    ). This report delineates the relations between NO\n                    <jats:sup>\u2022<\/jats:sup>\n                    and UVA in the human keratinocyte cell line HaCaT. NOS activity was stimulated by exposure of cells to L\u2010Arg just after irradiation. L\u2010Arg (5 mM) supply led to an increase in UVA (25.3 J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) cytotoxicity (% of viability 18 \u00b1 3%) whereas neither L\u2010Arg itself nor UVA irradiation induced cell death at the doses used in this study. Cells were also treated either with L\u2010thiocitrulline (L\u2010Thio), an irreversible inhibitor of NOS, or with exogenous superoxide dismutase (SOD) and catalase. L\u2010Thio and SOD prevented L\u2010Arg\u2010mediated deleterious effects in irradiated cells, whereas catalase was ineffective. Intracellular antioxidant enzyme activities were also determined. UVA\/L\u2010Arg stress altered catalase (66% decrease) and glutathione peroxidase (83% decrease). DNA damage was evaluated using the \u2018comet assay\u2019 and quantified using the \u2018tail moment\u2019. UVA alone was genotoxic (mean tail moment: 25.43 \u00b1 1.23,\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001 compared control cells). The addition of L\u2010Arg potentiated DNA damage (mean tail moment: 41.05\u00b13.9) whereas L\u2010Thio prevented them (mean tail moment 9.86 \u00b1 0.98). We attempted to assess the effect of poly(ADP\u2010ribose) polymerase (PARP) inhibition on cell death. Using the PARP inhibitor 3\u2010aminobenzamide, we established that PARP determines both cell lysis and DNA damage induced by UVA and\/or L\u2010Arg. Our findings demonstrated that L\u2010Arg was able to increase UVA\u2010mediated deleterious effects in keratinocytes (both DNA damage and cytotoxicity) and that the ratio NO\n                    <jats:sup>\u2022<\/jats:sup>\n                    \/O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2010<\/jats:sup>\n                    plays a key role in these processes.\u2014Didier, C., Emonet\u2010Piccardi, N., B\u00e9ani, J.\u2010C., Cadet, J., Richard, M.\u2010J. L\u2010arginine increases UVA cytotoxicity in irradiated human keratinocyte cell line: potential role of nitric oxide.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 13, 1817\u20131824 (1999)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.13.13.1817","type":"journal-article","created":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T04:42:55Z","timestamp":1516855375000},"page":"1817-1824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["L\u2010arginine increases UVA cytotoxicity in irradiated human keratinocyte cell line: potential role of nitric oxide"],"prefix":"10.1096","volume":"13","author":[{"given":"Christine","family":"Didier","sequence":"first","affiliation":[{"name":"LBSO\/LCR7 No. 8\u2010Universit\u00e9 Joseph Fourier  F\u201038043 Grenoble Cedex 03 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Emonet\u2010Piccardi","sequence":"additional","affiliation":[{"name":"LBSO\/LCR7 No. 8\u2010Universit\u00e9 Joseph Fourier  F\u201038043 Grenoble Cedex 03 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Claude","family":"B\u00e9ani","sequence":"additional","affiliation":[{"name":"LBSO\/LCR7 No. 8\u2010Universit\u00e9 Joseph Fourier  F\u201038043 Grenoble Cedex 03 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Cadet","sequence":"additional","affiliation":[{"name":"Laboratoire L\u00e9sions des Acides Nucl\u00e9iques' D\u00e9partement de Recherche Fondamentale sur la Mati\u00e8re Condens\u00e9e Service de Chimie Inorganique et Biologie CEA\/Grenoble  F\u201038054 Grenoble Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Jeanne","family":"Richard","sequence":"additional","affiliation":[{"name":"LBSO\/LCR7 No. 8\u2010Universit\u00e9 Joseph Fourier  F\u201038043 Grenoble Cedex 03 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1999,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199312303292706"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.2.1402"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3180789"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00084.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12343523"},{"key":"e_1_2_7_7_1","first-page":"931","article-title":"Nitric oxide, peroxynitrite and nitroso\u2010compounds formation by ultraviolet A (UVA) \u2010irradiated human squamous cell carcinoma: potential role of nitric oxide in cancer prognosis","volume":"15","author":"Villiotou V.","year":"1995","journal-title":"Anticancer Res."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12343428"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.4.1620"},{"issue":"446","key":"e_1_2_7_10_1","first-page":"451","article-title":"Peroxynitrite formation from macrophage\u2010derived nitric oxide","volume":"298","author":"Ischiropoulos H.","year":"1992","journal-title":"Arch. 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