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We quantified several components of the AC pathway in pregnant (P,\n                    <jats:italic>n<\/jats:italic>\n                    =21) and nonpregnant human myometria (NP,\n                    <jats:italic>n<\/jats:italic>\n                    =10). AC activity was ~sev\u2010enfold higher in P than in NP under basal and stimulated conditions (MnCl\n                    <jats:sub>2<\/jats:sub>\n                    \/GTP\/GTP + isopro\u2010terenol). In addition, relative stimulation (% of basal) by 5\u2019\u2010guanosine\u2010\u03b27\u2010iminotriphosphate and forskolin was twofold higher in P. \u03b2\u2010Adrenoceptor density was low and unaltered in P. Go\n                    <jats:sub>s<\/jats:sub>\n                    mRNA splice variants did not differ in P. Using antisera against different epitopes of Go\n                    <jats:sub>s<\/jats:sub>\n                    (carboxyl\u2010\/more amino\u2010terminal), we found unchanged expression of Go\n                    <jats:sub>s<\/jats:sub>\n                    short and long (45, 47 kDa) in P. Two additional proteins in P (51, 59 kDa) were detectable only by the carboxyl\u2010terminal antiserum and lacked GTP binding properties. The 59 kDa protein could be identified as a recently discovered cytoskeletal protein, smoothelin, which was 10\u2010fold increased in P. These data indicate that the apparent up\u2010regulation of large Go\n                    <jats:sub>s<\/jats:sub>\n                    species in P is mimicked by elevated smoothelin. Therefore, the increase in AC cannot be attributed to changes in Go\n                    <jats:sub>s<\/jats:sub>\n                    \u2010or \u03b2\u2010adrenoreceptors. Epitope sharing between Go\n                    <jats:sub>s<\/jats:sub>\n                    and smoothelin should be considered in experiments on smooth muscle tissues.\u2014Gsell, S., Eschenhagen, T., Kaspareit, G., Nose, M., Scholz, H., Behrens, O., Wieland, T. Apparent up\u2010regulation of stimulatory G\u2010protein \u03b1 subunits in the pregnant human myometrium is mimicked by elevated smoothelin expression.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    14, 17\u201326(2000)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.14.1.17","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T05:45:35Z","timestamp":1516254335000},"page":"17-26","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Apparent up\u2010regulation of stimulatory G\u2010protein \u03b1 subunits in the pregnant human myometrium is mimicked by elevated smoothelin expression\n                    <sup>1<\/sup>"],"prefix":"10.1096","volume":"14","author":[{"given":"STEFANIE","family":"GSELL","sequence":"first","affiliation":[{"name":"Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung f\u00fcr Pharmakologie Universit\u00e4ts\u2010Krankenhaus Eppendorf  20246 Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"THOMAS","family":"ESCHENHAGEN","sequence":"additional","affiliation":[{"name":"Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung f\u00fcr Pharmakologie Universit\u00e4ts\u2010Krankenhaus Eppendorf  20246 Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GRIT","family":"KASPAREIT","sequence":"additional","affiliation":[{"name":"Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung f\u00fcr Pharmakologie Universit\u00e4ts\u2010Krankenhaus Eppendorf  20246 Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MONIKA","family":"NOSE","sequence":"additional","affiliation":[{"name":"Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung f\u00fcr Pharmakologie Universit\u00e4ts\u2010Krankenhaus Eppendorf  20246 Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HASSO","family":"SCHOLZ","sequence":"additional","affiliation":[{"name":"Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung f\u00fcr Pharmakologie Universit\u00e4ts\u2010Krankenhaus Eppendorf  20246 Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"OLIVER","family":"BEHRENS","sequence":"additional","affiliation":[{"name":"Kreiskrankenhaus Rendsburg Gynakologische Abteilung  24768 Rendsburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"THOMAS","family":"WIELAND","sequence":"additional","affiliation":[{"name":"Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung f\u00fcr Pharmakologie Universit\u00e4ts\u2010Krankenhaus Eppendorf  20246 Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,1]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(00)89000-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1360497"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-71-5-1243"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0130023"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-70-5-1299"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0026-895X(25)13308-7","article-title":"Estrogen reduces beta\u2010adrenocep\u2010tor\u2010mediated cAMP production and the concentration of the guanyl nucleotide\u2010regulatory protein, Gs, in rabbit myometrium","volume":"33","author":"Riemer R. 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