{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:03:44Z","timestamp":1790755424028,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Regulation of bone formation is important in the pathogenesis of many conditions such as osteoporosis, fracture healing, and loosening of orthopedic implants. We have recently identified a novel rat cDNA\n                    <jats:italic>(best5)<\/jats:italic>\n                    by differential display PCR that is regulated during osteoblast differentiation and bone formation\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo.<\/jats:italic>\n                    Expression of\n                    <jats:italic>best5<\/jats:italic>\n                    mRNA is induced in cultures of osteoblasts by both interferon\u2010\u03b1 (IFN\u2010\u03b1) or IFN\u2010\u03b3. Whereas IFN\u2010\u03b1 induced a rapid, transient induction of\n                    <jats:italic>best5<\/jats:italic>\n                    expression peaking at 4\u20136 h poststimulation, IFN\u2010 \u03b3 elicited a more prolonged induction of\n                    <jats:italic>best5<\/jats:italic>\n                    expression, which remained elevated 48 h poststimulation. A polyclonal antibody generated to a peptide derived from the\n                    <jats:italic>best5<\/jats:italic>\n                    coding region recognized a 27 kDa protein on Western blot analysis of osteoblast ly\u2010sates. We localized BEST5 protein in osteoblast progenitor cells and mature osteoblasts in sections of rat tibiae and in sections of bones loaded\n                    <jats:italic>in vivo<\/jats:italic>\n                    to induce adaptive bone formation.\n                    <jats:italic>Best5<\/jats:italic>\n                    maytherefore be a fundamental intermediate in the response of osteoblasts to stimuli that modulate proliferation\/ differentiation, such as interferons or mechanical loading. These findings highlight the close interactions between the immune system and bone cells and may open new therapeutic avenues in modulating bone mass\u2014Grewal, T. S., Genever, P. G., Brabbs, A. C., Birch, M., Skerry, T. M.\n                    <jats:italic>Best5<\/jats:italic>\n                    : a novel interferon\u2010inducible gene expressed during bone formation.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    14, 523\u2013531 (2000)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.14.3.523","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T09:05:51Z","timestamp":1516266351000},"page":"523-531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["<i>Best5:<\/i>\n                    a novel interferon\u2010inducible gene expressed during bone formation"],"prefix":"10.1096","volume":"14","author":[{"given":"TARLOCHAN SINGH","family":"GREWAL","sequence":"first","affiliation":[{"name":"Department of Biology The University of York Heslington York YO10 5YW United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PAUL G.","family":"GENEVER","sequence":"additional","affiliation":[{"name":"Department of Biology The University of York Heslington York YO10 5YW United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALEX C.","family":"BRABBS","sequence":"additional","affiliation":[{"name":"Department of Biology The University of York Heslington York YO10 5YW United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARK","family":"BIRCH","sequence":"additional","affiliation":[{"name":"Department of Biology The University of York Heslington York YO10 5YW United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TIMOTHY M.","family":"SKERRY","sequence":"additional","affiliation":[{"name":"Department of Biology The University of York Heslington York YO10 5YW United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2000,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.4.537"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(90)90004-S"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(86)90134-9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/smvy.1995.0023"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.16.1.293"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19377.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.4049\/jimmunol.131.3.1300","article-title":"Comparative analysis of the influences of human gamma, alpha and beta interferons on human multipotential (CFU\u2010GEMM), erythroid (BFU\u2010e) and granulocyte\u2010macrophage (CFU\u2010GM) progenitor cells","volume":"131","author":"Broxmeyer H. 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