{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:43:08Z","timestamp":1774676588441,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1989,2,1]],"date-time":"1989-02-01T00:00:00Z","timestamp":602294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1989,2]]},"abstract":"\n Danazol, an attenuated androgen, is useful in endometriosis, idiopathic thrombocytopenic purpura (ITP), and autoimmune hemolytic anemia (AIHA). However, its mechanism of action is unknown. We investigated the possibility that danazol affects cell membranes directly. Red cell osmotic fragility was studied in patients receiving danazol. A significant decrease in osmotic fragility was observed. Accompanying the change, peripheral blood smears showed many target cells and electron microscopy revealed extra folds in erythrocyte membranes. Twenty\u2010two patients were studied prospectively before and after danazol. Osmotic fragility decreased significantly (\n P<\/jats:italic>\n < 0.001) in 1 month of therapy and progressed with further treatment. A rebound increase (\n P<\/jats:italic>\n < 0.01) was observed in 1 month after discontinuation of danazol among 16 patients. Incubation experiments showed that danazol\u2010induced changes are not reversed with normal sera. Patient sera did not induce the changes in normal red cells. Danazol in vitro protected red cells from osmotic lysis at low concentrations but enhanced lysis at high concentrations. We suggest that danazol alters red cell membranes directly to increase their surface area, inducing target cell formation and increasing their resistance to osmotic lysis.\u2014A\n hn<\/jats:sc>\n , Y. S.; K\n im<\/jats:sc>\n , C. I.; M\n ylvaganam<\/jats:sc>\n , R.; F\n ernandez<\/jats:sc>\n , L. F.; T\n emple<\/jats:sc>\n , J. D, J\n r<\/jats:sc>\n .; C\n ayer<\/jats:sc>\n , M. L.; H\n arrington<\/jats:sc>\n , W. J. Danazol therapy renders red cells resistant to osmotic lysis.\n FASEB J.<\/jats:italic>\n 3: 157\u2010162; 1989.\n <\/jats:p>","DOI":"10.1096\/fasebj.3.2.2914627","type":"journal-article","created":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T04:27:30Z","timestamp":1516854450000},"page":"157-162","source":"Crossref","is-referenced-by-count":16,"title":["Danazol therapy renders red cells resistant to osmotic lysis\n 1<\/sup>"],"prefix":"10.1096","volume":"3","author":[{"given":"Yeon S.","family":"Ahn","sequence":"first","affiliation":[{"name":"Department of Medicine U.S. Veterans Administration Hospital Department of Medicine Center for Blood Diseases University of Miami School of Medicine Miami Florida 33136"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis F.","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Department of Medicine U.S. Veterans Administration Hospital Department of Medicine Center for Blood Diseases University of Miami School of Medicine Miami Florida 33136"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chae I.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medicine U.S. Veterans Administration Hospital Department of Medicine Center for Blood Diseases University of Miami School of Medicine Miami Florida 33136"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravindra","family":"Mylvaganam","sequence":"additional","affiliation":[{"name":"Department of Medicine U.S. Veterans Administration Hospital Department of Medicine Center for Blood Diseases University of Miami School of Medicine Miami Florida 33136"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"J. Donald","family":"Temple","sequence":"additional","affiliation":[{"name":"Department of Medicine U.S. Veterans Administration Hospital Department of Medicine Center for Blood Diseases University of Miami School of Medicine Miami Florida 33136"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn L.","family":"Cayer","sequence":"additional","affiliation":[{"name":"Coulter Electronics Hialeah Florida 33014 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Harrington","sequence":"additional","affiliation":[{"name":"Department of Medicine U.S. Veterans Administration Hospital Department of Medicine Center for Blood Diseases University of Miami School of Medicine Miami Florida 33136"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,2]]},"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.3.2.2914627","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.3.2.2914627","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:43:09Z","timestamp":1761691389000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fasebj.3.2.2914627"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,2]]}},"alternative-id":["10.1096\/fasebj.3.2.2914627"],"URL":"https:\/\/doi.org\/10.1096\/fasebj.3.2.2914627","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,2]]}}}