{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T18:08:49Z","timestamp":1776103729875,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,1,1]],"date-time":"1990-01-01T00:00:00Z","timestamp":631152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1990,1]]},"abstract":"\n We combined biochemical measurements with novel techniques for image analysis in the rat femur to characterize the location and nature of the defect in mineralization known to occur in growing animals after spaceflight. Concentrations of mineral and osteocalcin were low in the distal half of the diaphysis and concentrations of collagen were low with evidence of increased synthesis in the proximal half of the diaphysis of the flight bones. X\u2010ray microtomography provided semiquantitative data in computer\u2010generated sections of whole wet bone that indicated a longitudinal gradient of decreasing mineralization toward the distal diaphysis, similar to the chemistry results. Analysis of embedded sections by backscattered electrons in a scanning electron microscope revealed distinct patterns of mineral distribution in the proximal, central, and distal regions of the diaphysis and also showed a net reduction in mineral levels toward the distal shaft. Increases in mineral density to higher fractions in controls were less in the flight bones at all three levels, with the most distal cross\u2010sectional area most affected. The combined results from these novel techniques identified the areas of femoral diaphysis most vulnerable to the mineralization defect associated with spaceflight and\/or the stress of landing.\u2014M\n echanic<\/jats:sc>\n , G. L.; A\n rnaud<\/jats:sc>\n , S. B.; B\n oyde<\/jats:sc>\n , A.; B\n romage<\/jats:sc>\n , T. G.; B\n uckendahl<\/jats:sc>\n , P.; E\n lliott<\/jats:sc>\n , J.; K\n atz<\/jats:sc>\n , E. P.; D\n urnova<\/jats:sc>\n , G. N. Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite.\n FASEB J.<\/jats:italic>\n 4: 34\u201040; 1990.\n <\/jats:p>","DOI":"10.1096\/fasebj.4.1.2295376","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T07:34:59Z","timestamp":1516260899000},"page":"34-40","source":"Crossref","is-referenced-by-count":36,"title":["Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite"],"prefix":"10.1096","volume":"4","author":[{"given":"Gerald L.","family":"Mechanic","sequence":"first","affiliation":[{"name":"Dental Research Center University of North Carolina Chapel Hill North Carolina 27514 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara B.","family":"Arnaud","sequence":"additional","affiliation":[{"name":"NASA Ames Research Center Moffett Field California 94035 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Boyde","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology University College London Gower Street London WC1E 6BT England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy G.","family":"Bromage","sequence":"additional","affiliation":[{"name":"Department of Child Dental Health The London Hospital Medical College Dental School Turner Street London E1 2AD England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Buckendahl","sequence":"additional","affiliation":[{"name":"Thimann Laboratories University of California Santa Cruz California 95064 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James C.","family":"Elliott","sequence":"additional","affiliation":[{"name":"Department of Child Dental Health The London Hospital Medical College Dental School Turner Street London E1 2AD England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elton P.","family":"Katz","sequence":"additional","affiliation":[{"name":"School of Dental Medicine University of Connecticut Health Science Center Farmington Connecticut 06032 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina N.","family":"Durnova","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Problems USSR Ministry of Health Moscow USSR"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,1]]},"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.4.1.2295376","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.4.1.2295376","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:46:45Z","timestamp":1761677205000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fasebj.4.1.2295376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1096\/fasebj.4.1.2295376"],"URL":"https:\/\/doi.org\/10.1096\/fasebj.4.1.2295376","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}