{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T07:10:53Z","timestamp":1761808253978,"version":"build-2065373602"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AA024284"],"award-info":[{"award-number":["AA024284"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chronic alcohol consumption increases the susceptibility to infectious diseases by compromising the immune system. Cytomegalovirus infection is common in humans and usually is asymptomatic in immunocompetent people. However, it can induce life\u2010threatening medical complications in immunocompromised individuals such as alcoholics. How chronic alcohol consumption exacerbates cytomegalovirus infection is not known. Herein, we used a mouse cytomegalovirus model to study the underlying cellular and molecular mechanism. We found that alcohol consumption increased viral titers in spleen after 4\u00a0days of infection, enhanced body weight loss and inhibited splenomegaly during the acute phase of infection. Blood level of IFN\u2010\u03b2, splenic IFN\u2010\u03b3 and granzyme B\u2010producing NK cells were lower in alcohol\u2010consuming mice than in water\u2010drinking mice at 12\u00a0hours after viral infection. Moreover, alcohol consumption decreased IL\u201015\u2010producing DC after 36\u00a0hours infection, inhibited NK cell, specifically Ly49H\n                    <jats:sup>+<\/jats:sup>\n                    NK cell maturation and proliferation 3\u20106\u00a0days after viral infection. Surprisingly, alcohol consumption enhanced NK cell and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell continuous activation and increased granzyme B\u2010producing cells. However, alcohol consumption decreased the expression of perforin in spleen and liver. Taken together, chronic alcohol consumption exacerbates cytomegalovirus infection via impairing nonspecific and specific NK cell activation, specifically IFN\u2010\u03b3 and perforin production.\n                  <\/jats:p>","DOI":"10.1096\/fba.1019","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T10:59:24Z","timestamp":1553511564000},"page":"18-31","source":"Crossref","is-referenced-by-count":2,"title":["Chronic alcohol consumption exacerbates murine cytomegalovirus infection via impairing nonspecific and specific NK activation in mice"],"prefix":"10.1096","volume":"1","author":[{"given":"Alex","family":"Little","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences College of Pharmacy and Pharmaceutical Sciences, Washington State University Spokane Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanfei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences College of Pharmacy and Pharmaceutical Sciences, Washington State University Spokane Washington"},{"name":"Department of Oncology The First Hospital of Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faya","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences College of Pharmacy and Pharmaceutical Sciences, Washington State University Spokane Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences College of Pharmacy and Pharmaceutical Sciences, Washington State University Spokane 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