{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T01:17:41Z","timestamp":1773969461071,"version":"3.50.1"},"reference-count":74,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01 DK091392"],"award-info":[{"award-number":["R01 DK091392"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["P30 DK079328"],"award-info":[{"award-number":["P30 DK079328"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alpha\u2010Klotho (\u03b1Klotho), produced by the kidney and selected organs, is essential for tissue maintenance and protection. Homozygous \u03b1Klotho\u2010deficiency leads to premature multi\u2010organ degeneration and death; heterozygous insufficiency leads to apoptosis, oxidative stress, and increased injury susceptibility. There is inconsistent data in the literature regarding whether \u03b1Klotho is produced locally in the lung or derived from circulation. We probed murine and human lung by immunohistochemistry (IHC) and immunoblot (IB) using two monoclonal (anti\u2010\u03b1Klotho Kl1 and Kl2 domains) and three other common commercial antibodies. Monoclonal anti\u2010Kl1 and anti\u2010Kl2 yielded no labeling in lung on IHC or IB; specific labeling was observed in kidney (positive control) and also murine lungs following tracheal delivery of \u03b1Klotho cDNA, demonstrating specificity and ability to detect artificial pulmonary expression. Other commercial antibodies labeled numerous lung structures (IHC) and multiple bands (IB) incompatible with known \u03b1Klotho mobility; labeling was not abolished by blocking with purified \u03b1Klotho or using lungs from hypomorphic \u03b1Klotho\u2010deficient mice, indicating nonspecificity. Results highlight the need for rigorous validation of reagents. The lung lacks native \u03b1Klotho expression and derives full\u2010length \u03b1Klotho from circulation; findings could explain susceptibility to lung injury in extrapulmonary pathology associated with reduced circulating \u03b1Klotho levels, for example, renal failure. Conversely, \u03b1Klotho may be artificially expressed in the lung, suggesting therapeutic opportunities.<\/jats:p>","DOI":"10.1096\/fba.2019-00016","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T12:24:27Z","timestamp":1568377467000},"page":"675-687","source":"Crossref","is-referenced-by-count":12,"title":["Alpha\u2010Klotho, a critical protein for lung health, is not expressed in normal lung"],"prefix":"10.1096","volume":"1","author":[{"given":"Jianning","family":"Zhang","sequence":"first","affiliation":[{"name":"Departments of Internal Medicine University of Texas Southwestern Medical Center Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khoa","family":"Cao","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine University of Texas Southwestern Medical Center Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanne V.","family":"Pastor","sequence":"additional","affiliation":[{"name":"Charles and Jane Pak Center of Mineral Metabolism and Clinical Research University of Texas Southwestern Medical Center Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Li","sequence":"additional","affiliation":[{"name":"Charles and Jane Pak Center of Mineral Metabolism and Clinical Research University of Texas Southwestern Medical Center Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orson W.","family":"Moe","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine University of Texas Southwestern Medical Center Dallas TX USA"},{"name":"Charles and Jane Pak Center of Mineral Metabolism and Clinical Research University of Texas Southwestern Medical Center Dallas TX USA"},{"name":"Departments of Physiology University of Texas Southwestern Medical Center Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie C. 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