{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T18:12:14Z","timestamp":1760551934461,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T00:00:00Z","timestamp":1670803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012033","name":"Japanese Dermatological Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005926","name":"Cosmetology Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005926","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Xeroderma pigmentosum (XP) is a hereditary disorder characterized by photosensitivity, predisposition to skin cancers, and neurological abnormalities including microcephaly and progressive neurodegeneration. A lack of nucleotide excision repair (NER) in patients with XP can cause hypersensitivity to the sun, leading to skin cancer, whereas the etiology of the neuronal symptoms of XP remains ambiguous. There are various neurological disorders that perturb neuronal migration, causing mislocalization and disorganization of the cortical lamination. Here, we investigated the role of the XP group\u2010A (<jats:italic>Xpa<\/jats:italic>) gene in directed cell migration. First, we adopted an in utero electroporation method to transduce shRNA vectors into the murine embryonic cerebral cortex for the in vivo knockdown of <jats:italic>Xpa<\/jats:italic>. <jats:italic>Xpa\u2010<\/jats:italic>knockdown neurons in the embryonic cerebral cortex showed abnormal cell migration, cell cycle exit, and differentiation. The genotype\u2013phenotype relationship between the lack of XPA and cell migration abnormalities was confirmed using both a scratch assay and time\u2010lapse microscopy in XP\u2010A patient\u2010derived fibroblasts. Unlike healthy cells, these cells showed impairment in overall mobility and the direction of motility. Therefore, abnormal cell migration may explain neural tissue abnormalities in patients with XP\u2010A.<\/jats:p>","DOI":"10.1096\/fba.2022-00084","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T08:41:47Z","timestamp":1669106507000},"page":"53-61","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cell migration is impaired in <scp>XPA<\/scp>\u2010deficient cells"],"prefix":"10.1096","volume":"5","author":[{"given":"Seiji","family":"Takeuchi","sequence":"first","affiliation":[{"name":"Division of Dermatology, Department of Internal Related Kobe University Graduate School of Medicine  Kobe Japan"},{"name":"Division of Cell Biology and Neuroscience Department of Morphological and Physiological Sciences, Faculty of Medical Sciences University of Fukui  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0364-711X","authenticated-orcid":false,"given":"Takeshi","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Internal Related Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Takemori","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Internal Related Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoaki","family":"Saito","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology, Biosignal Research Center Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikako","family":"Nishigori","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Internal Related Kobe University Graduate School of Medicine  Kobe Japan"},{"name":"Department of iPS cell applications Graduate School of Medicine, Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6805-8351","authenticated-orcid":false,"given":"Makoto","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Cell Biology and Neuroscience Department of Morphological and Physiological Sciences, Faculty of Medical Sciences University of Fukui  Fukui Japan"},{"name":"Department of Anatomy and Neuroscience Graduate School of Medicine, Osaka University  Osaka Japan"},{"name":"United Graduate School of Child Development Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui (UGSCD)  Osaka 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