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Telomere protection protein 1 (TPP1) is one of the main six subunits of the shelterin complex supporting the telomere stability and genomic integrity. Dysfunctional telomeres and shelterin complex are associated as a disease mechanism of tobacco smoke\u2010induced pulmonary damage and disease processes. The airway epithelium is critical to maintaining respiratory homeostasis and is implicated in lung diseases. Club cells (also known as clara cells) play an essential role in the immune response, surfactant production, and metabolism. Disrupted shelterin complex may lead to dysregulated cellular function, DNA damage, and disease progression. However, it is unknown if the conditional removal of TPP1 from Club cells can induce lung disease pathogenesis caused by tobacco smoke exposure. In this study, conditional knockout of Club\u2010cell specific TPP1 demonstrated the instability of other shelterin protein subunits, such as TRF1, dysregulation of cell cycle checkpoint proteins, p53 and downstream targets, and dysregulation of telomeric genes. This was associated with age\u2010dependent senescence\u2010associated genes, increased DNA damage, and upregulated RANTES\/IL13\/IL33 mediated lung inflammation and injury network by cigarette smoke (CS). These phenomena are also associated with alterations in cytochrome P450 and glutathione transferases, upregulated molecular pathways promoting lung lesions, bronchial neoplasms, and adenocarcinomas. These findings suggest a pivotal role of TPP1 in maintaining lung homeostasis and injurious responses in response to CS. Thus, these data TPP1 may have therapeutic value in alleviating telomere\u2010related chronic lung diseases.<\/jats:p>","DOI":"10.1096\/fba.2023-00115","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T05:14:26Z","timestamp":1705295666000},"page":"53-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Club cell\u2010specific telomere protection protein 1 (<scp>TPP1<\/scp>) protects against tobacco smoke\u2010induced lung inflammation, xenobiotic metabolic dysregulation, and injurious responses"],"prefix":"10.1096","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4648-9529","authenticated-orcid":false,"given":"Thivanka","family":"Muthumalage","sequence":"first","affiliation":[{"name":"Department of Environmental Medicine, School of Medicine and Dentistry University of Rochester Medical Center  Rochester New York 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