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Defects in male counterpart lead to failure of conception so are outcomes of assisted reproduction affected by quality of sperm. Microfluidics, deals with minute volumes (\u03bcL) of liquids run in small\u2010scale microchannel networks in the form of laminar flow streamlines. Microfluidic sperm selection designs have been developed in chip formats, mimicking in\u00a0vivo situations. Here sperms are selected and analyzed based on motility and sperm behavioral properties. Compared to conventional sperm selection methods, this selection method enables to produce high\u2010quality motile sperm cells possessing non\u2010damaged or least damaged DNA, achieve greater success of insemination in bovines, and achieve enhanced pregnancy rates and live births in assisted reproduction\u2014in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Besides, the concentration of sperm available to oocyte can be controlled by regulating the flow rate in microfluidic chips. The challenges in this technology are commercialization of chips, development of fully functional species\u2010specific microfluidic tools, limited number of studies available in literature, and need of thorough understanding in reproductive physiology of domestic animals. In conclusion, incorporation of microfluidic system in assisted reproduction for sperm selection may promise a great success in IVF and ICSI outcomes. Future prospectives are to make this technology more superior and need to modify chip designs which is cost effective and species specific and ready for commercialization. Comprehensive studies in animal species are needed to be carried out for wider application of microfluidic sperm selection in in\u00a0vitro procedures.<\/jats:p>","DOI":"10.1096\/fba.2024-00041","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T05:43:38Z","timestamp":1724391818000},"page":"406-423","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Microfluidics\u2014A novel technique for high\u2010quality sperm selection for greater\n                    <scp>ART<\/scp>\n                    outcomes"],"prefix":"10.1096","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2562-9350","authenticated-orcid":false,"given":"Ghulam Rasool","family":"Bhat","sequence":"first","affiliation":[{"name":"Division of Animal Reproduction, Gynaecology and Obstetrics Sher\u2010e\u2010Kashmir Institute of Agricultural Sciences and Technology of Kashmir  Srinagar 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