{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:16:03Z","timestamp":1761675363287,"version":"build-2065373602"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study characterizes a fluorescent\n                    <jats:italic>Slc17a6<\/jats:italic>\n                    \u2010tdTomato neuronal reporter mouse line with strong labeling of axons throughout the optic nerve, of retinal ganglion cell (RGC) soma in the ganglion cell layer (GCL), and of RGC dendrites in the inner plexiform layer (IPL). The model facilitated assessment of RGC loss in models of degeneration and of RGC detection in mixed neural\/glial cultures. The tdTomato signal showed strong overlap with &gt;98% cells immunolabeled with RGC markers RBPMS or BRN3A, consistent with the ubiquitous presence of the vesicular glutamate transporter 2 (VGUT2, SLC17A6) in all RGC subtypes. There was no cross\u2010labeling of ChAT\u2010positive displaced amacrine cells in the GCL, although some signal emanated from the outer plexiform layer, consistent with horizontal cells. The fluorescence allowed rapid screening of RGC loss following optic nerve crush and intraocular pressure (IOP) elevation. The bright fluorescence also enabled non\u2010invasive monitoring of extensive neurite networks and neuron\/astrocyte interactions in culture. Robust Ca\n                    <jats:sup>2+<\/jats:sup>\n                    responses to P2X7R agonist BzATP were detected from fluorescent RGCs using Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010indicator Fura\u20102. Fluorescence from axons and soma was detected in\u00a0vivo with a confocal scanning laser ophthalmoscope (cSLO); automatic RGC soma counts enhanced through machine learning approached the numbers found in retinal wholemounts. Controls indicated no impact of\n                    <jats:italic>Slc17a6<\/jats:italic>\n                    \u2010tdTomato expression on light\u2010dependent neuronal function as measured with a microelectrode array (MEA), or on retinal structure as measured with optical coherence tomography (OCT). In summary, the bright fluorescence in axons, dendrites and soma of ~all RGCs in the\n                    <jats:italic>Slc17a6<\/jats:italic>\n                    \u2010tdTomato reporter mouse may facilitate the study of RGCs.\n                  <\/jats:p>","DOI":"10.1096\/fba.2024-00095","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:45:04Z","timestamp":1734396304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fluorescent identification of axons, dendrites and soma of neuronal retinal ganglion cells with a genetic marker as a tool for facilitating the study of neurodegeneration"],"prefix":"10.1096","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1717-9962","authenticated-orcid":false,"given":"Puttipong","family":"Sripinun","sequence":"first","affiliation":[{"name":"Department of Basic and Translational Science University of Pennsylvania  Philadelphia 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