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CAP\u2010p5 capability to induce hydroxyapatite crystal formation on demineralized dentin blocks was characterized by scanning electron microscopy, \u03bcRAMAN, and high\u2010resolution transmission electron microscopy. The results revealed that CAP\u2010p5 promoted cell proliferation and cell differentiation and increases alkaline phosphatase activity of HPLC and mineralization at an optimal concentration of 10\u2009\u03bcg\/mL. It induced the expression of cementum molecular markers BSP, CAP, CEMP1, and ALP at the protein level. In a cell\u2010free system, human demineralized dentin blocks coated with CAP\u2010p5 induced the deposition of a homogeneous and continuous mineralized layer, intimately integrated with the underlying dentin indicating new cementum formation. Physicochemical characterization of this mineral layer showed that it is composed of hydroxyapatite crystals. Demineralized dentin blocks coated with CAP\u2010p5 implanted subcutaneously in BALB\/cAnNCrl were analyzed histologically; the results disclosed that CAP\u2010p5 could induce the deposition of a cementum layer intimately integrated with the subjacent dentin with cementocytes embedded into the cementum matrix. Immunostaining showed the expression of cementum molecular markers; <jats:italic>v.gr.<\/jats:italic> BSP, CAP, CEMP1 and ALP, validating the molecular identity of the newly deposited cementum. We conclude that CAP\u2010p5 is a new biomolecule with the potential of therapeutic application to contribute to the regeneration of cementum and periodontal structures lost in periodontal disease.<\/jats:p>","DOI":"10.1096\/fba.2024-00119","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T12:40:42Z","timestamp":1735562442000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cementum attachment protein\u2010derived peptide induces cementum formation"],"prefix":"10.1096","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8284-0745","authenticated-orcid":false,"given":"L\u00eda Hoz","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Periodontal, Facultad de Odontolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8810-9091","authenticated-orcid":false,"given":"Maricela Santana","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Periodontal, Facultad de Odontolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3383-7873","authenticated-orcid":false,"given":"Luis Fernando","family":"Ram\u00edrez Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Periodontal, Facultad de Odontolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9376-7347","authenticated-orcid":false,"given":"Gonzalo Montoya","family":"Ayala","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Periodontal, Facultad de Odontolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8447-6425","authenticated-orcid":false,"given":"Sonia L\u00f3pez","family":"Letayf","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Periodontal, Facultad de Odontolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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