{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T20:22:43Z","timestamp":1779394963501,"version":"3.53.1"},"reference-count":76,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:00:00Z","timestamp":1748217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alternative splicing (AS) is a highly conserved posttranscriptional mechanism, generating mRNA variants to diversify the proteome. Acute endurance exercise appears to transiently perturb AS in skeletal muscle, but transcriptome\u2010wide responses are not well defined. We aimed to better understand differential AS (DAS) and differential isoform expression (DIE) in skeletal muscle by comparing short\u2010read (SRS) and long\u2010read RNA sequencing (LRS) data. Publicly accessible SRS of clinical exercise studies were extracted from the Gene Expression Omnibus. Oxford Nanopore LRS was performed on mouse gastrocnemius before and following treadmill exercise (30\u2009m running,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095 mice\/group, 20 total, 10\u2009weeks old). Differential gene expression (DGE) and DIE were analyzed and validated using RT\u2010PCR and immunoblots. Both SRS and LRS illustrated significant DGE in skeletal muscle postexercise, including 89 RNA\u2010binding proteins (RBPs). rMATS analysis of SRS revealed that exon\u2010skipping and intron\u2010retaining events were the most common. Swan analysis of LRS revealed several common genes across postexercise cohorts with significant DAS but no DGE: 13 exercise\u2010associated genes, including\n                    <jats:italic>mSirt2<\/jats:italic>\n                    (24.5% shift at 24\u2009h postexercise [24pe],\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.005); 61 RBPs, including\n                    <jats:italic>mHnrnpa3<\/jats:italic>\n                    (28.5% at 24pe,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.02),\n                    <jats:italic>mHnrnpa1<\/jats:italic>\n                    (30.6% at 24pe,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.004), and\n                    <jats:italic>mTia1<\/jats:italic>\n                    (53.6% at 24pe,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.004). We illustrated that acute endurance exercise can elicit changes in AS\u2010related responses and RBP expression in skeletal muscle, especially at 24pe. SRS is a powerful tool for analyzing DGE but lacks isoform detection, posing a major gap in knowledge of \u201chidden\u201d genes with no transcriptional but significant DIE and protein expression changes. Additionally, LRS can uncover previously unknown transcript diversity and mechanisms influencing endurance exercise adaptations and responses.\n                  <\/jats:p>","DOI":"10.1096\/fba.2025-00007","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T11:44:18Z","timestamp":1748259858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Acute Endurance Exercise on Alternative Splicing in Skeletal Muscle"],"prefix":"10.1096","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7865-2061","authenticated-orcid":false,"given":"Alexander","family":"Ahn","sequence":"first","affiliation":[{"name":"School of Kinesiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6959-8544","authenticated-orcid":false,"given":"Jeongjin J.","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Kinesiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2082-4827","authenticated-orcid":false,"given":"Aaron L.","family":"Slusher","sequence":"additional","affiliation":[{"name":"School of Kinesiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey Y.","family":"Ying","sequence":"additional","affiliation":[{"name":"School of Kinesiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7975-0303","authenticated-orcid":false,"given":"Eric Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Kinesiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0423-4800","authenticated-orcid":false,"given":"Andrew T.","family":"Ludlow","sequence":"additional","affiliation":[{"name":"School of Kinesiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,26]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Adult Physical 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