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However, its effects on corneal nerve and epithelium repair are not well understood. We performed in\u00a0vitro and in\u00a0vivo studies to assess the effects of Netrin\u20104 in corneal wound healing. We found that Netrin\u20104 induced extensive neurite growth and branching in trigeminal ganglia neurons and accelerated the scratch closure of corneal epithelial cells. In\u00a0vivo, the dual action of Netrin\u20104 enhanced corneal epithelium healing and nerve regeneration in mice subjected to corneal epithelium debridement. Inhibition studies demonstrate that Netrin\u20104\u2010induced neuronal growth may be mediated by interaction with Neogenin or \u03b16\u03b21 Integrin receptors. In conclusion, our data demonstrate that Netrin\u20104 has trophic and neuroregenerative effects in the cornea and could be a suitable therapeutic target to treat corneal injury.<\/jats:p>","DOI":"10.1096\/fba.2025-00064","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T09:19:32Z","timestamp":1747214372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Netrin\u20104 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve Regeneration"],"prefix":"10.1096","volume":"7","author":[{"given":"Cissy","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Illinois\u2010Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Lara\u2010Newman","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Illinois\u2010Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Illinois\u2010Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark I.","family":"Rosenblatt","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Illinois\u2010Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0429-7692","authenticated-orcid":false,"given":"Victor H.","family":"Guaiquil","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Illinois\u2010Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200105000-00008"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(03)00050-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70112-X"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2019.107709"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2005.07.027"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2015.113"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072165"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(14)70257-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.151415"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.165555"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6448-2_1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.04.012"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4695(200008)44:2<219::AID-NEU11>3.0.CO;2-W"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065572"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191962"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.2337\/db18-1172"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-9760"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7417.1"},{"key":"e_1_2_10_20_1","first-page":"2219","article-title":"Roles of Eph\/Ephrin Bidirectional Signaling in Central Nervous System Injury and Recovery","volume":"15","author":"Yang J. 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