{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:34:00Z","timestamp":1791030840523,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Microgravity exposure affects both tissues and cells, and, in this regard, one of the most affected targets is the skeletal muscle system due to the significant loss of bone and muscle mass leading to osteoporosis and sarcopenia, respectively. Several efforts are underway to counteract the effects of microgravity, and recent studies on irisin, a myokine with anabolic effects on the musculoskeletal system, have shown promising results. Due to the practical challenges of conducting experiments in actual microgravity, different devices generating a simulated microgravity condition on Earth have been developed. Here, we exposed myoblasts, osteoblasts, osteocytes to a random position machine (RPM) for five days to assess microgravity effect on the expression of key differentiation factors in cells untreated or treated with irisin. In myoblasts (C2C12), exposure to RPM led to increased expression of early myogenesis maker genes\n                    <jats:italic>Pax7<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0016),\n                    <jats:italic>Myf5<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0005) and\n                    <jats:italic>MyoD<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0009). Irisin treatment in the last 8\u2009h of RPM cultures prevented these increases by returning\n                    <jats:italic>Pax7<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0008) and\n                    <jats:italic>MyoD<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.01) to control values, and only partially\n                    <jats:italic>Myf5<\/jats:italic>\n                    . In bone cells, exposure to RPM for 5\u2009days showed no effect in osteoblasts (MC3T3) but decreased the expression of\n                    <jats:italic>Pdpn<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0285) and\n                    <jats:italic>Dmp\u20101<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0423) genes in osteocytes (MLO\u2010Y4). Irisin treatment completely prevented the decline in\n                    <jats:italic>Pdpn<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.293) and\n                    <jats:italic>Dmp\u20101<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0339) levels. Overall, our data showed that the impact of RPM exposure keeps myoblasts and osteocytes in a proliferative state, and irisin treatment restores them to their baseline biological condition, suggesting that irisin can counteract the changes induced by simulated microgravity.\n                  <\/jats:p>","DOI":"10.1096\/fba.2025-00085","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T06:34:32Z","timestamp":1752215672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Irisin Prevents the Effects of Simulated Microgravity on Bone and Muscle Differentiation Markers"],"prefix":"10.1096","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-3350","authenticated-orcid":false,"given":"Lorenzo","family":"Sanesi","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5660-1747","authenticated-orcid":false,"given":"Roberta","family":"Zerlotin","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9136-6722","authenticated-orcid":false,"given":"Alessio","family":"Campiolo","sequence":"additional","affiliation":[{"name":"Cellular Oncology Unit IRCCS Ospedale Policlinico San Martino  Genoa Italy"},{"name":"Department of Experimental Medicine (DIMES) University of Genoa  Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0881-6840","authenticated-orcid":false,"given":"Angela","family":"Oranger","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2728-3034","authenticated-orcid":false,"given":"Manuela","family":"Dicarlo","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7774-9749","authenticated-orcid":false,"given":"Clelia","family":"Suriano","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8735-4420","authenticated-orcid":false,"given":"Ameneh","family":"Ghadiri","sequence":"additional","affiliation":[{"name":"Cellular Oncology Unit IRCCS Ospedale Policlinico San Martino  Genoa Italy"},{"name":"Department of Experimental Medicine (DIMES) University of Genoa  Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8501-5399","authenticated-orcid":false,"given":"Graziana","family":"Colaianni","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7121-5899","authenticated-orcid":false,"given":"Maria","family":"Grano","sequence":"additional","affiliation":[{"name":"Department of Precision and Regenerative Medicine and Ionian Area University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1478-4910","authenticated-orcid":false,"given":"Sara","family":"Tavella","sequence":"additional","affiliation":[{"name":"Cellular Oncology Unit IRCCS Ospedale Policlinico San Martino  Genoa Italy"},{"name":"Department of Experimental Medicine (DIMES) University of Genoa  Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9970-3539","authenticated-orcid":false,"given":"Silvia","family":"Colucci","sequence":"additional","affiliation":[{"name":"Department of Translational Biomedicine and Neuroscience University of Bari  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/life4040621"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1249\/00003677-199001000-00007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.204.18.3201"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.188508"},{"issue":"11","key":"e_1_2_9_6_1","first-page":"1209","article-title":"Long\u2010Term Follow\u2010Up of Skylab Bone Demineralization","volume":"51","author":"Tilton F. 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