{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T07:23:06Z","timestamp":1765610586613,"version":"3.46.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP\u2010130286"],"award-info":[{"award-number":["MOP\u2010130286"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102016\u201006639"],"award-info":[{"award-number":["RGPIN\u20102016\u201006639"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FASEB BioAdvances"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    High\u2010fat diets (HFDs) are a well\u2010established cause of hepatic steatosis, a condition associated with altered hepatic fatty acid metabolism and reduced choline availability. Folic acid (FA) deficiency can also promote steatosis, in part by impairing choline metabolism. Although FA supplementation has been found to reduce liver fat in mice with hepatic steatosis, it is unclear if this effect is due to increased hepatic choline levels, changes in fatty acid profiles, or a combination of both. In this study, four\u2010week\u2010old male C57BL\/6J mice were fed 45\u2009kcal% HFDs with total FA content adjusted to onefold, fivefold, or tenfold AIN\u201093G recommended level (2\u2009mg\/kg diet) for 15\u2009weeks ad\u00a0libitum. Hepatic triacylglycerol (TAG), choline concentrations, expression of key genes in choline metabolism, and TAG\u2010bound fatty acid profiles were analyzed. Mice receiving tenfold FA had lower liver weight and hepatic TAG levels compared to the onefold control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Both fivefold and tenfold FA supplementation increased hepatic choline concentrations and upregulated mRNA expression of choline\u2010metabolizing genes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), suggesting enhanced choline utilization. Additionally, tenfold FA supplementation altered the hepatic TAG fatty acid profile, reducing levels of palmitoleic acid and oleic acid (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), fatty acids typically associated with\n                    <jats:italic>de novo<\/jats:italic>\n                    lipogenesis. A strong inverse correlation was observed between hepatic choline and TAG levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001, adjusted\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.56), supporting a potential role for choline availability in mediating FA's protective effects. Folic acid supplementation protects against hepatic steatosis by enhancing choline availability, modulating lipid metabolism, and reducing liver fat accumulation.\n                  <\/jats:p>","DOI":"10.1096\/fba.2025-00251","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:28:02Z","timestamp":1761722882000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Folic Acid Supplementation Attenuates Hepatic Steatosis by Enhancing Choline Availability and Remodeling Fatty Acid Profiles in Mice Fed a High\u2010Fat Diet"],"prefix":"10.1096","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5127-0489","authenticated-orcid":false,"given":"Eva","family":"Kranenburg","sequence":"first","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4341-7757","authenticated-orcid":false,"given":"Ruslan","family":"Kubant","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2752-3073","authenticated-orcid":false,"given":"Zeyu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3388-1372","authenticated-orcid":false,"given":"Adam H.","family":"Metherel","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1099-4198","authenticated-orcid":false,"given":"Chuck T.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1805-8844","authenticated-orcid":false,"given":"Jacqueline L.","family":"Beaudry","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9733-2516","authenticated-orcid":false,"given":"Clara E.","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences College of Biological Science, University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3246-0642","authenticated-orcid":false,"given":"Perlina","family":"Vaz","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9613-6225","authenticated-orcid":false,"given":"G. Harvey","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"},{"name":"Department of Physiology Temerty Faculty of Medicine, University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2024.103043"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807616200"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-10-234"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/dsi23-0013"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxaa032"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2023.1141156"},{"issue":"1","key":"e_1_2_10_8_1","first-page":"1","article-title":"Serum Folate Associated With Nonalcoholic Fatty Liver Disease and Advanced Hepatic Fibrosis","volume":"13","author":"Chen H. 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