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Human tonsil\u2010derived T and B lymphocytes express Cx40 and 43; circulating human T, B, and NK lymphocytes express Cx43 and directly transfer between each other a low molecular dye indicative that functional gap junctions exist. We now identify specific properties in the immune system underwritten by gap junctions. Mixed lymphocytes cultured in the presence of two reagents with independent inhibitory action on gap junction communication, a connexin mimetic peptide and 18\u2010\u03b1\u2010glycyrrhetinic acid, markedly reduced the secretion of IgM, IgG, and IgA. The secretion of these immunoglobulins by purified B cells was also reduced by the two classes of gap junction inhibitors. Complex temporal inhibitory effects on the expression of mRNA encoding interleukins, especially IL\u201010, were also observed. The results indicate that intercellular signaling across gap junctions is an important component of the mechanisms underlying metabolic cooperation in the immune system.\u2014Oviedo\u2010Orta, E., Gasque, P., Evans, W. H. Immunoglobulin and cytokine expression in mixed lymphocyte cultures is reduced by disruption of gap junction intercellular communication.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    15, 768\u2010774 (2001)\n                  <\/jats:p>","DOI":"10.1096\/fj.00-0288com","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T19:03:24Z","timestamp":1027710204000},"page":"768-774","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Immunoglobulin and cytokine expression in mixed lymphocyte cultures is reduced by disruption of gap junction intercellular communication"],"prefix":"10.1096","volume":"15","author":[{"given":"ERNESTO","family":"OVIEDO\u2010ORTA","sequence":"first","affiliation":[{"name":"Wales Heart Research Institute Heath Park Cardiff CF14 4XN Wales U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PHILIPPE","family":"GASQUE","sequence":"additional","affiliation":[{"name":"University of Wales College of Medicine Department of Medical Biochemistry Cardiff CF14 4XN Wales U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"HOWARD EVANS","sequence":"additional","affiliation":[{"name":"Wales Heart Research Institute Heath Park Cardiff CF14 4XN Wales U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,3]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"178","article-title":"Adhesion molecules in B\u2010chronic lymphoproliferative disorders","volume":"36","author":"Angelopoulou M. 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