{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:58:54Z","timestamp":1791089934368,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sera from patients with chronic Chagas heart disease recognize the carboxyl\u2010terminal regions of the\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    ribosomal P proteins defined by B cell epitopes P013 (EDDDDDFGMGALF) and R13 (EEEDDDMGFGLFD) corresponding to the\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    ribosomal P0 (TcP0) and P2\u00df (TcP2\u00df) proteins, respectively. It has been hypothesized that both epitopes may induce antibodies that cross\u2010react and stimulate the \u00df 1\u2010adrenoreceptor. However, no proof as to their pathogenicity has been obtained. We investigated the consequences of immunizing mice with either TcP0 or TcP2\u00df proteins. Of 24 immunized animals, 16 generated antibodies against the carboxyl\u2010terminal end of the corresponding protein, 13 of which showed an altered ECG (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001, 81%). Immunization with TcP0 induced anti\u2010P013 antibodies that bind to and stimulate cardiac G\u2010protein\u2010coupled receptors and are linked to the induction of supraventricular arrhythmia, repolar\u2010ization, and conduction abnormalities as monitored by serial electrocardiographic analysis. In contrast, immunization with TcP2\u00df generated anti\u2010R13 antibodies with an exclusive \u00df1\u2010adrenergic\u2010stimulating activity whose appearance strictly correlated with the recording of supraventricular tachycardia and death. These findings demonstrate that anti\u2010P antibodies are arrhythmogenic in the setting of a normal heart, since no inflammatory lesions or fibrosis were evident to light microscopic examination.\u2014Lopez Bergami, P., Scaglione, J., Levin, M. J. Antibodies against the carboxyl\u2010terminal end of the\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    ribosomal P proteins are pathogenic.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    15, 2602\u20132612 (2001)\n                  <\/jats:p>","DOI":"10.1096\/fj.01-0132com","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T19:03:24Z","timestamp":1027710204000},"page":"2602-2612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Antibodies against the carboxyl\u2010terminal end of the\n                    <i>Trypanosoma cruzi<\/i>\n                    ribosomal P proteins are pathogenic"],"prefix":"10.1096","volume":"15","author":[{"given":"Pablo L\u00f3pez","family":"Bergami","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas Instituto de Investigaciones en Ingenier\u00eda Genetica y Biolog\u00eda Molecular (INGEBI) 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Scaglione","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas Instituto de Investigaciones en Ingenier\u00eda Genetica y Biolog\u00eda Molecular (INGEBI) 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano Jorge","family":"Levin","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas Instituto de Investigaciones en Ingenier\u00eda Genetica y Biolog\u00eda Molecular (INGEBI) 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"95","volume-title":"Arrhytmia Management in Chagas Disease","author":"Elizari M. 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