{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:27:12Z","timestamp":1791268032480,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2001,10,15]],"date-time":"2001-10-15T00:00:00Z","timestamp":1003104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IBN-049087"],"award-info":[{"award-number":["IBN-049087"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We identified four new isoforms of human CRH\u2010R1 (e\u2010h) and three of mouse (mCRH\u2010R1c, e, and f). In all new forms exon 6 was missing. Human CRH\u2010R1e was characterized by the deletion of exons 3 and 4; exon 12 from CRH\u2010R1f; exon 11, 27 base pairs (bp) of exon 10 and 28 bp of exon 12 from CRH\u2010R1g and CRH\u2010R1h by the addition of a cryptic exon. In mouse CRH\u2010R1c exon 3 was spliced out; in mCRH\u2010R1e exons 3 and 4 and in mCRH\u2010R1f exon 11 were spliced from mRNA. CRH\u2010R1 was expressed in all skin specimens in patterns dependent on the cell type, physiological status, and presence of pathology. CRH\u2010R1\u03b1, the most prevalent form, was detected in almost all samples. Ultraviolet radiation (UV) changed the splicing pattern and induced or increased expression of CRH\u2010R1\u03b1 in cultured skin cells. Continuing UV treatment of succeeding generations of cells resulted in a progressive increase in the number of CRH\u2010R1 isoforms, which suggests that receptor heterogeneity might favor cell survival. TPA (phorbol 12\u2010myristate 13\u2010acetate), forskolin, dbcAMP (N6, 2\u2032\u2010O\u2010dibutyryladenosine 3\u2032:5\u2032\u2010cyclic monophospate sodium), and IBMX (3\u2010isobutyl\u20101\u2010methylxanthine) also changed the splicing pattern. We suggest that a polymorphism of CRH\u2010R1 expression is related to anatomic location, skin physiological or pathologic status, specific cell type, and external stress (UV), and that cAMP\u2010dependent pathways and TPA may regulate CRH\u2010R1.<\/jats:p>","DOI":"10.1096\/fj.01-0487fje","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T19:03:24Z","timestamp":1027710204000},"page":"1-24","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":146,"title":["Alternative splicing of CRH\u2010R1 receptors in human and mouse skin: identification of new variants and their differential expression"],"prefix":"10.1096","volume":"15","author":[{"given":"Alexander","family":"Pisarchik","sequence":"first","affiliation":[{"name":"Department of Pathology 576 BMH University of Tennessee Health Science Center 899 Madison Ave Memphis Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej T.","family":"Slominski","sequence":"additional","affiliation":[{"name":"Department of Pathology 576 BMH University of Tennessee Health Science Center 899 Madison Ave Memphis Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,10,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08687.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.19.8967"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.1.8536644"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90230-O"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.2969"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.836"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80427-V"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.9.5.637"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.4.1108"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69041640.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.1.7879"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(96)81594-X"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(98)00037-X"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00322-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2884"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.13.12.0391"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.2.5757"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1999.0720388.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2071"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.3.979"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.21.5.457"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08686.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.85.2.815"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0850rev"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1290\/1071-2690(2001)037<0050:PEOCRH>2.0.CO;2"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1290\/1071-2690(2000)036<0211:CRHARP>2.0.CO;2"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(95)00159-X"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(92)90347-R"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-999-0093-6"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1789"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(92)90047-F"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.24.14519"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.11.7.9946"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01604-2"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00128-8"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/85613"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/35057062"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80444-4"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.2.237"},{"key":"e_1_2_6_41_1","volume-title":"Human CRF2 receptor protein, its production and use","author":"Hinuma K.","year":"1997"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-202X(93)90507-E"},{"key":"e_1_2_6_43_1","volume-title":"Tumor Cell Heterogeneity","author":"Owens A. H.","year":"1982"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1992.tb00561.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(98)00023-6"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01315-4"},{"key":"e_1_2_6_47_1","first-page":"3575","article-title":"Liquid chromatography-mass spectrometry detection of corticotropin-releasing hormone and proopiomelanocortin-derived peptides in human skin","volume":"85","author":"Slominski A.","year":"2000","journal-title":"J. Clin. Endocrinol. Metab."}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.01-0487fje","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.01-0487fje","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.01-0487fje","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:32:36Z","timestamp":1761701556000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.01-0487fje"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,15]]},"references-count":46,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1096\/fj.01-0487fje"],"URL":"https:\/\/doi.org\/10.1096\/fj.01-0487fje","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,15]]},"assertion":[{"value":"2001-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}