{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T23:41:52Z","timestamp":1794613312124,"version":"4.9.1"},"reference-count":31,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2002,10,18]],"date-time":"2002-10-18T00:00:00Z","timestamp":1034899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We attempted establishing an\n                    <jats:italic>in vitro<\/jats:italic>\n                    coculture system by using human dermal papilla cells (DPCs) from androgenetic alopecia (AGA) and keratinocytes (KCs) to explore the role of androgens in hair growth regulation. Androgen showed no significant effect on the growth of KCs when they were cocultured with DPCs from AGA. Because the expressions of mRNA of androgen receptor (AR) decreased during subcultivation of DPCs\n                    <jats:italic>in vitro<\/jats:italic>\n                    , we transiently transfected the AR expression vector into the DPCs and cocultured them with KCs. In this modified coculture, androgen significantly suppressed the growth of KCs by ~50%, indicating that overexpression of AR can restore the responsiveness of the DPCs to androgen\n                    <jats:italic>in vivo<\/jats:italic>\n                    . We found that androgen stimulated the expression of TGF\u2010\u03b21 mRNA in the cocultured DPCs. ELISA assays demonstrated that androgen treatment increased the secretion of both total and active TGF\u2010\u03b21 in the conditioned medium. Moreover, the neutralizing anti\u2010TGF\u2010\u03b21 antibody reversed the androgen\u2010elicited growth inhibition of KCs in a dose\u2010dependent manner. These findings suggest that androgen\u2010inducible TGF\u2010\u03b21 derived from DPCs of AGA is involved in epithelial cell growth suppression in our coculture system, providing the clue to understand the paradoxical effects of androgens for human hair growth.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0043fje","type":"journal-article","created":{"date-parts":[[2002,12,5]],"date-time":"2002-12-05T12:43:29Z","timestamp":1039092209000},"page":"1967-1969","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":203,"title":["Androgen\u2010inducible TGF\u2010\u03b21 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understanding paradoxical effects of androgen on human hair growth"],"prefix":"10.1096","volume":"16","author":[{"given":"Shigeki","family":"Inui","sequence":"first","affiliation":[{"name":"Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine Osaka University  Suita Osaka 565\u20130871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Fukuzato","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine Osaka University  Suita Osaka 565\u20130871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine Osaka University  Suita Osaka 565\u20130871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine Osaka University  Suita Osaka 565\u20130871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Itami","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine Osaka University  Suita Osaka 565\u20130871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,10,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0300-595X(86)80028-7","article-title":"Hair follicles and associated glands are androgen targets","volume":"15","author":"Ebiling F. 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