{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:29:21Z","timestamp":1761762561510,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2002,8,7]],"date-time":"2002-08-07T00:00:00Z","timestamp":1028678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Hepatitis B virus (HBV) is a major risk factor for hepatocellular carcinoma (HCC). HBV encodes an oncogenic HBx gene that functions as a transcriptional coactivator of multiple cellular genes. To understand the role(s) of HBx in the early genesis of HCC, we systematically analyzed gene expression profiles by serial analysis of gene expression (SAGE) in freshly isolated human primary hepatocytes infected with a replication\u2010defective adenovirus containing HBx. A total of 19,501 sequence tags (representing 1443 unique transcripts) were analyzed, which provide a distribution of a transcriptome characteristic of normal hepatocytes and a profile associated with HBx expression. Examples of the targeted genes were confirmed by the Megarray analysis with a significant correlation between quantitative SAGE and Megarray (r = 0.8, P&gt; 0.005). In HBx\u2010expressing hepatocytes, a total of 57 transcripts (3.9%) were induced, and 46 transcripts (3.3%) were repressed by more than fivefold. Interestingly, most of the HBxup\u2010regulated transcripts can be clustered into three major classes, including genes that encode ribosomal proteins, transcription factors with zinc\u2010finger motifs, and proteins associated with protein degradation pathways. These results suggest that HBx may function as a major regulator in common cellular pathways that, in turn, regulate protein synthesis, gene transcription, and protein degradation.<\/jats:p>","DOI":"10.1096\/fj.02-0074fje","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T19:22:27Z","timestamp":1034191347000},"page":"1665-1667","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["SAGE transcript profiles of normal primary human hepatocytes expressing oncogenic hepatitis B virus X protein"],"prefix":"10.1096","volume":"16","author":[{"given":"Chuan\u2010Ging","family":"Wu","sequence":"first","affiliation":[{"name":"Laboratory of Human Carcinogenesis, Center for Cancer Research National Cancer Institute  Bethesda MD 20892\u20134255"},{"name":"Division of Hematology Center for Biologies Evaluation and Research, Food and Drug Administration  Bethesda MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marshonna","family":"Forgues","sequence":"additional","affiliation":[{"name":"Laboratory of Human Carcinogenesis, Center for Cancer Research National Cancer Institute  Bethesda MD 20892\u20134255"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shabina","family":"Siddique","sequence":"additional","affiliation":[{"name":"Laboratory of Human Carcinogenesis, Center for Cancer Research National Cancer Institute  Bethesda MD 20892\u20134255"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Farnsworth","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Medical College of Virginia  Richmond VA 23298\u20130058"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristoffer","family":"Velerie","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Medical College of Virginia  Richmond VA 23298\u20130058"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Human Carcinogenesis, Center for Cancer Research National Cancer Institute  Bethesda MD 20892\u20134255"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,8,7]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1942","article-title":"Hepatitis B virus","volume":"61","author":"Beasley R. 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